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EC number: 202-269-1 | CAS number: 93-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976-01-28
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable for reliability but not in detail documented. Study report meets basic scientific principles. Study was conducted prior to GLP and OECD guideline implementation.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- no
- Remarks:
- GLP-guidelines not yet in force at date of the study
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2'-chloroacetoacetanilide
- EC Number:
- 202-269-1
- EC Name:
- 2'-chloroacetoacetanilide
- Cas Number:
- 93-70-9
- Molecular formula:
- C10H10ClNO2
- IUPAC Name:
- 2'-chloroacetoacetanilide
- Details on test material:
- Physical state: solid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Fasted (24 hours) albino rats were used in this study. Weights 200 - 300 grams.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- propylene glycol
- Details on oral exposure:
- The product under test was placed in a glass syringe and introduced through the esophagus into the stomach with a stainless steel catheter.
- Doses:
- 6 dose groups (males & females) + control group (males & females) with dosages of:
- 4'000 mg/kg
- 8'000 mg/kg
- 10'000 mg/kg
- 12'500 mg/kg
- 16'000 mg/kg
- 32'000 mg/kg - No. of animals per sex per dose:
- 5 per sex per dose
5 per sex per control group - Control animals:
- yes
- Details on study design:
- A group of approximately 70 albino male and female rats, fasted for twenty-four hours were employed to establish an LD50 range for each product
under test.
Young adult rats which had not been used for previous test purposes were assigned to various dose levels at random. Both sexes were equally
distributed.
The product under test was placed in a glass syringe and introduced through the esophagus into the stomach with a stainless steel catheter.
Animals on the same dosage level were then placed in a common cage with free access to food and water. The animals were observed daily for a
two week period.
No postmortem, or histopathology examinations were performed in this particular study - Statistics:
- No data available.
Results and discussion
- Preliminary study:
- No preliminary test performed.
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD0
- Effect level:
- 4 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 11 600 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 9 700 - 14 000
- Sex:
- male
- Dose descriptor:
- LD100
- Effect level:
- 16 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD0
- Effect level:
- 10 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 12 400 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 11 100 - 13 900
- Sex:
- female
- Dose descriptor:
- LD100
- Effect level:
- 16 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality was observed at dose concentrations of 4000 mg/kg in males. Animals were found dead at 8000 mg/kg on day 4 and above (>8000 -
32000 mg/kg) in male rats.
No mortality was observed at dose concentrations of 4000 - 10000 mg/kg in females. Animals were found dead at 12500 mg/kg and above (>12500 -32000 mg/kg) in female rats.
Details are given in the table below. - Clinical signs:
- other: Male rats: unkempt coats were noted for 36-48 hours at 4000 mg/kg. At levels of 8000 mg/kg, 10000 mg/kg and 12500 mg/kg, lethargy was accompanied with spasmodic movements, nasal hemorrhage and unkempt coats. Survivors appeared normal by the ninth day. Th
- Gross pathology:
- No Postmortem, or histopathology examinations were performed in this particular study.
- Other findings:
- No other findings.
Any other information on results incl. tables
Mortality:
Dose Level (mg/kg) |
No. of deaths (males) |
Number of deaths (females) |
4000 |
0 |
0 |
8000 |
1 (at day 4) |
0 |
10000 |
1 (at day 1) |
0 |
12500 |
3 (2 at day 1 & 1 at day 6) |
3 (2 at day 1 & 1 at day 2) |
16000 |
5 (2 at day 1; 1 at day 2; 1 at day 3 & 1 at day 5)) |
5 (at day 1) |
32000 |
5 (at day 1) |
5 (at day 1) |
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information
- Conclusions:
- The LD50 for male albino rats is 11600 mg/kg bw and for female albino rats 12400 mg/kg bw in the acute toxicity study.
- Executive summary:
The study was performed 1976, before GLP- and OECD-testing guidelines were available and in force. A group of approximately 70 albino male and female rats, fasted for twenty-four hours were employed to establish an LD50 range for each product under test. Young adult rats which had not been used for previous test purposes were assigned to various dose levels at random. Both sexes were equally distributed. Body weight of the rats were 200- 300 grams at the beginning of the study. The product under test was placed in a glass syringe and introduced through the esophagus into the stomach with a stainless steel catheter. Animals on the same dosage level were then placed in a common cage with free access to food and water. The animals were observed daily for a two week period. No postmortem, or histopathology examinations were performed in this particular study.
1. Clinical observations:
Unkempt coats were noted in male and female rats dosed at 4000 mg/kg and sluggish, impaired locomotion in females dosed at 4000 and 8000 mg/kg. Lethargy was accompanied with spasmodic movements, tremors and nasal hemorrhage in male in female rats dosed at 8000 mg/kg - 12500 mg/kg. The animals dosed at 16000 mg/kg were extremely lethargic, almost comatose, with spasmodic movements, nasal hemorrhage
and unkempt coats. Comas and oculo-nasal hemorrhage preceded death at 32000 mg/kg.
2. Mortality:
Death animals were observed at dose levels of 8000 mg/kg and above; for details refer to the above mentioned table.
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