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Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report date:
1976

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
yes
Remarks:
(48 hour scoring not performed, 10/14 hour dark/light rhythm, treatment on intact and abraded skin, occlusive treatment, 24 hour long treatment)
Principles of method if other than guideline:
Draize test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
O,O,O-triphenyl phosphorothioate
EC Number:
209-909-9
EC Name:
O,O,O-triphenyl phosphorothioate
Cas Number:
597-82-0
Molecular formula:
C18H15O3PS
IUPAC Name:
O,O,O-triphenyl thiophosphate

Test animals

Species:
rabbit
Strain:
other: Russian breed
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: no data
- Weight at study initiation: 1.5 - 2 kg
- Housing: individually in metal cages
- Diet: ad libitum; standard rabbit food (NAFAG Gossau SG)
- Water: ad libitum
- Acclimation period: 8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18 ± 1
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 10/14

Test system

Type of coverage:
occlusive
Preparation of test site:
other: shaved and abraded
Vehicle:
unchanged (no vehicle)
Controls:
other: untreated parts of the same rabbit
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied: 0.5 g
Duration of treatment / exposure:
24 hours
Observation period:
Scoring of skin irritation was performed 24 and 72 hours after initiation of treatment. Animals were observed until the reversal of irritation effects.
Number of animals:
6
Details on study design:
TEST SITE
- Two days before treatment the entire back, and the flank of the rabbits were shaved and immediately before treatment, the shaven skin of the left side was slightly scarified. The right side was not scarified.
- Area of exposure: 2.5 x 2.5 cm
- Type of wrap if used: The patches were covered with an impermeable material and fastened with adhesive tape.

REMOVAL OF TEST SUBSTANCE
- Washing: no
- Time after start of exposure: 24 hours

SCORING SYSTEM: draize system

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
other: 24 and 72 hours after start of treatment
Score:
1
Max. score:
4
Reversibility:
fully reversible within: 72 hours after start of treatment
Remarks on result:
other: 48h not investigated
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
other: 24 and 72 hours after start of treatment
Score:
1
Max. score:
4
Reversibility:
fully reversible within: 72 hours after start of treatment
Remarks on result:
other: 48h not investigated
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
other: 24 and 72 hours after start of treatment
Score:
0.5
Max. score:
4
Reversibility:
fully reversible within: 72 hours after start of treatment
Remarks on result:
other: 48h not investigated
Irritation parameter:
erythema score
Basis:
animal #4
Time point:
other: 24 and 72 hours after start of treatment
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
other: 48h not investigated
Irritation parameter:
erythema score
Basis:
animal #5
Time point:
other: 24 and 72 hours after start of treatment
Score:
0.5
Max. score:
4
Reversibility:
fully reversible within: 72 hours after start of treatment
Remarks on result:
other: 48h not investigated
Irritation parameter:
erythema score
Basis:
animal #6
Time point:
other: 24 and 72 hours after start of treatment
Score:
0.5
Max. score:
4
Reversibility:
fully reversible within: 72 hours after start of treatment
Remarks on result:
other: 48h not investigated
Irritation parameter:
edema score
Basis:
animal #1
Time point:
other: 24 and 72 hours after start of treatment
Score:
0.5
Max. score:
4
Reversibility:
fully reversible within: 72 hours after start of treatment
Remarks on result:
other: 48h not investigated
Irritation parameter:
edema score
Basis:
animal: 2, 3, 4 and 6
Time point:
other: 24 and 72 hours after start of treatment
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
other: 48h not investigated
Irritation parameter:
edema score
Basis:
animal #5
Time point:
other: 24 and 72 hours after start of treatment
Score:
0.5
Max. score:
4
Reversibility:
fully reversible within: 72 hours after start of treatment
Remarks on result:
other: 48h not investigated
Other effects:
Effects on scarified skin 24 hours after start of exposure (6 animals)
- Erythema: 2 (each of 5 animals), 1 (1 animal)
- Edema: 1 (each of 4 animals), 0 (2 animals)

Effects on scarified skin 72 hours after start of exposure (6 animal)
- Erythema: 1 (each of 4 animals), 0 (2 animals)
- Edema: 0 (all animals)

Any other information on results incl. tables

Only results of the non scarified skin sections are adopted in the final assessment for skin irritation.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met