Methyl chloroacetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP and method similar to OECD 471.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroacetate

EC Number:

202-501-1

EC Name:

Methyl chloroacetate

Cas Number:

96-34-4

Molecular formula:

C3H5ClO2

IUPAC Name:

methyl 2-chloroacetate

Details on test material:

- Name of test material (as cited in study report): Monochloressigsäuremethylester
- Physical state: Colourless liquid
- Analytical purity: 99.4%
- Lot/batch No.: KW 0727267-5 vom 28.10.82
- Storage condition of test material: Dark at 20ºC

Method

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium TA 100, TA 98, TA 1535, TA 1537, TA 1538 and Escherichia coli WP2uvrA

Metabolic activation:

with and without

Metabolic activation system:

S-9 mix

Test concentrations with justification for top dose:

0, 4, 20, 100, 500, 2500 and 5000 µg/plate (dissolved in 100 µl DMSO)

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Controls 1

Negative solvent / vehicle controls:

yes

Controls 2

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

without metabolic activation: TA100 and TA1535

Controls 3

Positive controls:

yes

Positive control substance:

9-aminoacridine

Remarks:

without metabolic activation: TA1537

Controls 4

Positive controls:

yes

Positive control substance:

2-nitrofluorene

Remarks:

without metabolic activation: TA98 and TA1538

Controls 5

Positive controls:

yes

Positive control substance:

other: N-methyl-N´-nitro-N-nitrosoguanidine

Remarks:

without metabolic activation: WP2uvrA

Controls 6

Positive controls:

yes

Positive control substance:

benzo(a)pyrene

Remarks:

with metabolic activation: TA98, TA100, TA1535, TA1537, TA1538 and WP2uvrA

Controls 7

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

with metabolic activation: TA98, TA100, TA1535, TA1537, TA1538 and WP2uvrA

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation);

DURATION
- Exposure duration: 48 to 72 hours at 37 ºC in the dark

NUMBER OF REPLICATIONS: Triplicates

DETERMINATION OF CYTOTOXICITY
- Method: Thinning of the bacterial lawn and reduction on the number of colonies. The solvent control is compared with the number of colonies per plate in the presence of the test compound (surviving factor).

Results and discussion

Test results

Species / strain:

other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538 and E. coli WP2uvrA

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

At doses of 2500 µg/plate

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.

Executive summary:

The test substance methyl chloroacetate was tested for mutagenicity with the strains TA98, TA100, TA1535, TA1537 and TA1538 of Salmonella typhimurium and WP2uvrA of E.coli. The mutagenicity studies were performed in the absence and in the presence of metabolic activation. A dose range of six concentrations from 4 µg/plate to 5000 µg/plate was used. Control plates and positive controls were also included in the study. The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.