Benzoic acid, 2,3,4,5-tetrachloro-6-cyano-, methyl ester, reaction products with p-phenylenediamine and sodium methoxide

Repeated dose toxicity: dermal

Administrative data

Endpoint:

short-term repeated dose toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

Only short summary available

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1964

Report date:

1964

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Test to study local effects of the test substance on rat skin

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-(1,4-phenylenediimino)bis[4,5,6,7-tetrachloro-1H-isoindol-1-one]

EC Number:

226-999-5

EC Name:

3,3'-(1,4-phenylenediimino)bis[4,5,6,7-tetrachloro-1H-isoindol-1-one]

Cas Number:

5590-18-1

Molecular formula:

C22H6Cl8N4O2

IUPAC Name:

3,3'-(1,4-phenylenediimino)bis(4,5,6,7-tetrachloro-1H-isoindol-1-one)

Constituent 2

Reference substance name:

mono/bis-methoxy derivatives

Molecular formula:

C22-24H6-12Cl6-8N4O2-4

IUPAC Name:

mono/bis-methoxy derivatives

Test material form:

solid: bulk

Specific details on test material used for the study:

- Name of test material (as cited in study report): GP 43692/I
- Physical state: no data

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 126 - 143 g, mean 134 g
- no further data

ENVIRONMENTAL CONDITIONS
not reported

Administration / exposure

Type of coverage:

open

Vehicle:

other: aqueous suspension with Gummi arabicum

Details on exposure:

TEST SITE
- Area of exposure: back, ca. 20 cm2
- Type of wrap if used: open conditions; animals were restrained in small cages during the exposure treatments
- Time intervals for shavings or clipplings: once before the start of the treatment

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: 3 h

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.4cm^3/animal (= ca. 3 cm^3/kg bw)
- Concentration (if solution): 5%
- Constant volume or concentration used: no data

USE OF RESTRAINERS FOR PREVENTING INGESTION: yes

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

4 weeks; for 3 hours per day

Frequency of treatment:

daily 5 days/week

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

0.4 other: cm^3/animal

Remarks:

5 % aqueous solution with gummi arabicum

Doses / concentrations 2

Dose / conc.:

150 mg/kg bw/day

Remarks:

Basis: nominal per unit body weight

No. of animals per sex per dose:

5

Control animals:

no

Examinations

Observations and examinations performed and frequency:

Effects on skin were recorded 24 hrs after the previous treatment i.e. just before the next treatment; weighing was performed before and at the end of the study; no further information given, however, it can be deduced from the summary of results that resorption effects were also studied.

Sacrifice and pathology:

no data

Results and discussion

Results of examinations

Clinical signs:

not specified

Dermal irritation:

no effects observed

Description (incidence and severity):

Local effects: no effects observed
Resorptive effects: no effects observed (no further data)

Mortality:

not specified

Body weight and weight changes:

effects observed, non-treatment-related

Description (incidence and severity):

Mean body weight increased from 134 g on day 0 to 221 g on day 28 (176 - 286 g)

Food consumption and compound intake (if feeding study):

not specified

Food efficiency:

not specified

Water consumption and compound intake (if drinking water study):

not specified

Ophthalmological findings:

not specified

Haematological findings:

not specified

Clinical biochemistry findings:

not specified

Endocrine findings:

not specified

Urinalysis findings:

not specified

Behaviour (functional findings):

not specified

Immunological findings:

not specified

Organ weight findings including organ / body weight ratios:

not specified

Gross pathological findings:

not specified

Neuropathological findings:

not specified

Histopathological findings: non-neoplastic:

not specified

Histopathological findings: neoplastic:

not specified

Other effects:

not specified

Effect levels

1

Dose descriptor:

NOAEL

Remarks:

local effects

Effect level:

ca. 150 mg/kg bw/day (nominal)

Based on:

test mat.

Sex:

male/female

Basis for effect level:

other: Highest dose test without local effects

2

Dose descriptor:

NOAEL

Remarks:

systemic effects

Effect level:

ca. 150 mg/kg bw/day (nominal)

Based on:

test mat.

Sex:

male/female

Basis for effect level:

other: only body weight gain was determined; result: normal body weight gain; no details about study observation program

Target system / organ toxicity

Critical effects observed:

not specified