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EC number: 700-972-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 11 to 15 November, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- (according to OECD, EC German principles of GLP)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 0.625, 1.25, 2.50, 5.0 and 10.0 mg/L
- Sampling method: The test substance concentration levels 2.5, 5.0 and 10.0 mg/L and the control were analytically verified via analysis of dissolved organic carbon (DOC) at test initiation. - Vehicle:
- no
- Details on test solutions:
- PREPARATION TEST SOLUTION: Test concentrations were prepared by dilution of a stock solution at 62.5 mg/L.
- Dispersion treatment: 1 min with Ultraturrax at 20000 rpm
- Vehicle: Dilution water - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: Aquarium AM Aegi, Feldstr. 7, D-30159 Hannover
- Feeding during test: No
ACCLIMATION
- Acclimation period: At least 12 d
- Temperature: 23 ± 2°C
- Light intensity: Diffuse light (0.1 - 10 µmol x m (2) x s (1), natural photoperiod).
- Food: Trouvit 40/2; Milkivit, D-86666, Bürgheim. The amount of food was 4% of the fish body weight per feeding day.
- Feeding frequency: 3 times per week
- Water (maintenance): Tap water. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine. The water was analysed once a year according to German tap water regulation. Nominal water parameters were:
Total hardness: 40 - 180 mg CaCO3/L
pH-value: 6.0 - 8.5
Dissolved oxygen: >80% of the air saturation value
The water was changed at least once per week.
- Health: No disease treatments were administered throughout holding. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 86 mg/L as CaCO3
- Test temperature:
- 21.0 - 22.7°C
- pH:
- 7.14-7.64
- Dissolved oxygen:
- 92-100%
- Nominal and measured concentrations:
- - Nominal: 0, 0.625, 1.25, 2.50, 5.0 and 10.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria loosely covered by glass tops
- Test volume: 10 L/vessel.
- Aeration: Gentle aeration was provided.
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Fish density in the vessel: < 1 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Same tap water was used for holding and testing. Detail of holding water is provided under ‘Details on test organisms’ section above.
- Intervals of water quality measurement: pH value, temperature and oxygen saturation were measured in all vessels at the beginning of test and every 24 h. Total hardness of the water was determined at the beginning of test from the control vessel. During the test the room temperature was recorded continuously with a thermo-hygrograph.
OTHER TEST CONDITIONS
- Photoperiod: A natural photoperiod was maintained during the test.
- Light intensity: 0.1 – 10.0 µmol x m (-2) x s (-1).
EFFECT PARAMETERS MEASURED: The test fish were observed after 24, 48, 72 and 96 h for the following endpoints:
1. Mortality (evident by no visible movement and if touching of the caudal peduncle produced no reaction)
2. Visible abnormalities (e.g. loss of equilibrium, swimming behavior, respiratory function, pigmentation etc).
3. Body weight of fish
4. Fish size
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: A 72 h non-GLP range finding test was carried out with five fish/concentration.
- Test concentrations: 0, 1.0 and 10.0 mg/L
- Results used to determine the conditions for the definitive study: Yes. In the range finding study, 100% mortality was observed at 10.0 mg/L dose level within 24 h of exposure. No mortality was observed at the remaining test concentration throughout the range finding study. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 5.1 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 4.5-5.7 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1.25 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: Slowed escape reflex, lying on its side, lethargy, staggering, hyperventilation were observed in animals at concentrations above 1.25 mg/L.
- Effect concentrations exceeding solubility of substance in test medium: The test substance was clearly dissolved at all concentration levels. Slight formation of foam was observed at 10 mg/L on Days 0-2. - Sublethal observations / clinical signs:
Table 1: Cumulative mortality data in the range-finding study
Nominal concentration (mg/L)
Cumulative Mortality (n=5)
24 h
48 h
72 h
Control
0
0
0
1.0
0
0
0
10
100
100
100
Table 2: Cumulative mortality data in the definitive study
Nominal concentration (mg/L)
Cumulative Mortality [ %]
24 h
48 h
72 h
96 h
Control
0
0
0
0
0.625
0
0
0
0
1.25
0
0
0
0
2.5
0
0
0
0
5.0
0
14
14
43
10
57
100
100
100
Table 3: Results based on the mortality data in the definitive study
Time (h)
LC50 (mg/L)
95% Confidence Limits (mg/L)
24
9.7
8.5->10
48
5.8
5.3-6.5
72
5.8
5.3-6.5
96
5.1
4.5-5.7
- Validity criteria fulfilled:
- yes
- Remarks:
- (O2 saturation was ≥ 60%, mortality in control was 0%)
- Conclusions:
- Under the conditions of the study, the nominal 96 h LC50 and NOEC were calculated to be 5.1 (95% CI: 4.5-5.7) and 1.25 mg/L, respectively.
- Executive summary:
A study was conducted to determine the acute toxicity of the test substance, C18-unsatd. DEA, to Zebra fish (Danio rerio) according to OECD Guideline 203, in compliance with GLP. Seven fish in single replicates were exposed to the test substance (dispersed in water by Ultraturrax) at nominal concentrations of 0, 0.625, 1.25, 2.50, 5.00 and 10.0 mg/L for 96 h under static conditions. Mortality was observed at 24, 48, 72 and 96 h. The dose levels of 2.5, 5.0 and 10.0 mg/L and the control were analytically verified via analysis of Dissolved Organic Carbon (DOC) at test initiation. Under the conditions of the study, the nominal 96 h LC50 and NOEC were calculated to be 5.1 (95% CI: 4.5-5.7) and 1.25 mg/L, respectively (Scheerbaum, 2002).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.1 mg/L
Additional information
A study was conducted to determine the acute toxicity of the test substance, C18-unsatd. DEA, to Zebra fish (Danio rerio) according to OECD Guideline 203, in compliance with GLP. Seven fish in single replicates were exposed to the test substance (dispersed in water by Ultraturrax) at nominal concentrations of 0, 0.625, 1.25, 2.50, 5.00 and 10.0 mg/L for 96 h under static conditions. Mortality was observed at 24, 48, 72 and 96 h. The dose levels of 2.5, 5.0 and 10.0 mg/L and the control were analytically verified via analysis of Dissolved Organic Carbon (DOC) at test initiation. Under the conditions of the study, the nominal 96 h LC50 and NOEC were calculated to be 5.1 (95% CI: 4.5-5.7) and 1.25 mg/L, respectively (Scheerbaum, 2002).
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