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EC number: 454-210-6 | CAS number: 13106-24-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 454-210-6
- EC Name:
- -
- Cas Number:
- 13106-24-6
- Molecular formula:
- C13 H30 N . C H3 O4 S
- IUPAC Name:
- tributyl(methyl)azanium methyl sulfate
- Details on test material:
- Substance number: 97/272; batch number: 601
Degree of purity/content: 101 g/ 100 g (H-NMR)
Date of manufacturing: June 1997
Physical state/appearance: Solid (congealed melt), white
Storage conditions: Room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Strain: Wistar (chbb: thom (SPF))
Origin: Dr. K. Thomae GmbH, Biberach, FRG
Age of the animals: Young adult animals.
Animal weights at start of the study: Animals of comparable weight; (150g -300g) (+/- 20% of the mean weight).
Acclimatization period: Acclimatization for at least 1 week.
Room temperature relative humidity: The animals were housed in fully air-conditioned rooms. Central air-conditioning guaranteed a range of 20 - 24 degrees centigrade for temperature and of 30 - 70 % for relative humidity. There were no deviations from these ranges, which influenced the results of the study.
Day/night rhythm: 12 h/12 h (6.00 a.m. - 6.00 p.m./ 6.00 p.m. - 6.00 a.m.)
Type of cage: Stainless steel wire mesh cages, type DK-III (Becker & Co., Castrop-Rauxel, FRG)
No. of animals per cage: Single housing.
Animal identification: Individual identification using cage cards and group identification by tail marking.
Bedding: No bedding in the cages; sawdust in the waste trays.
Drinking water: Tap water ad libitum per day.
Diet: Kliba-Labordiaet, Klingentalmuehle AG, Kaiseraugst, Switzerland, ad libitum.
Analysis of drinking water: The drinking water is regularly assayed for chemical contaminants by the municipal authorities of Frankenthal and the technical services of BASF Aktiengesellschaft as well as for the presence of germs by a contract laboratory.
Analysis of feed: The feed used in the study was assayed for chemical and microbiological contaminants.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- Fasting period: The animals were given no feed at least 16 hours before administration, but water was available ad libitum.
Reason for the vehicle: Aqueous formulation corresponds to the physiological medium.
Concentration: 20000 mg/ml
Administration volume: 10 ml/kg bw - Doses:
- Dose: 2000 mg/kg bw
- No. of animals per sex per dose:
- 3
- Details on study design:
- Time of day of administration: In the morning
Observation period: 14 days
Body weight determination: Individual body weights shortly before application (day 0), weekly thereafter and at the end of the study.
Signs and symptoms: Recording of signs and symptoms several times on the day of administration, at least once each workday for the individual animals; these records are maintained with the raw data.
Mortality: A check for any dead or moribund animal was made twice each workday and once on saturdays, sundays and on public holidays.
Pathology: Necropsy at the last day of the observation period. Withdrawal of food at least 16 houre before killing with CO2; then necropsy with grosspathology examination. Necropsy of all animals that died before as early as possible.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- Male: 0/3
Female: 1/3 (after 2 h; lt can not be excluded that the death was induced by gavage error.) - Clinical signs:
- other: Signs of toxicity noted in 1 female animal of the 2000 mg/kg dose group (after 2 h) comprised poor general state, dyspnoea, abdominal position, atonia and diarrhea. In the other animals (2000 mg/kg; 3 males, 2 females) no signs of toxicity were seen.
- Gross pathology:
- Animal that died (no. 382):
Lung: Discolouration, diffuse, in all lobes, dark red, moderate
Caecum: Dilatation, moderate, contents liquid
Animals that were sacrificed (3 males, 2 females): organs without particular findings.
Any other information on results incl. tables
Table 1: Bodyweights (g) of 3 male and female animals at day 0, 7, and 13 after application.
Male | day 0 | day 7 | day 13 |
cage 375 | 196 | 278 | 341 |
cage 376 | 200 | 272 | 323 |
cage 377 | 196 | 261 | 307 |
Female | |||
cage 381 | 186 | 221 | 235 |
cage 382 | 185 | ||
cage 383 | 185 | 217 | 226 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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