3-diethylaminophenol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study, no chemical control analysis

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-diethylaminophenol

EC Number:

202-090-9

EC Name:

3-diethylaminophenol

Cas Number:

91-68-9

Molecular formula:

C10H15NO

IUPAC Name:

3-(diethylamino)phenol

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The product was added to the test water without any pretreatment. Undissolved test material was visible at the bottom of the aquaria with an increase in the concentration.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Forellenhof Fredelsloh, Dr. med. vet. Otto Müller, D-3413 Moringen 1
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 6.6. cm
- Weight at study initiation (mean and range, SD): 6.1 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: about 10 weeks
- Acclimation conditions (same as test or not): 12 - 22 °C Water temperature
- Type and amount of food: Growing Feed F/B 50 ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 0 % during the last 2 weeks of housing

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mMol/L total hardness

Test temperature:

11-13 °C

pH:

7.8-8.1

Dissolved oxygen:

10.6-11.8 mg/L

Nominal and measured concentrations:

0, 1.0, 1.8, 3.2, 5.6, 10.0 mg/L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: Glas aquaria with stainless steel frame (80 x 35 x 46 cm)
- Type (delete if not applicable): open
- fill volume: 100 L volume
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38 412

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality, symptoms of intoxications

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.6 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.