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EC number: 207-306-5 | CAS number: 460-19-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Remarks:
- The artificial soil was obtained by mixing 12.5% peat, 5% vermiculite, 20% top soil and 62.5% Florida sand
- Total exposure duration:
- 14 d
- Test temperature:
- 20 +/- 2 °C
- pH:
- 5.9
- Moisture:
- 35 %
- Details on test conditions:
- One litre capacity soil jars were induced with cyanogen in the lab according to the study protocol. All jars were capped with butyl rubber septa. All ethanedinitrile treated jars were ventilated after 24 hours of application. The jars were opened after 12 hours of ventilation to count the number of live and dead earthworms. After counting, all materials were replaced in the jars. The jars were put under the light at room temperature 20 ± 2o C, soil moisture was adjusted when necessary, and mortality rate was recorded again on days 7 and 14.
- Nominal and measured concentrations:
- Seven rates at 0, 0.01, 0.1, 1.0, 10, 100 and 1,000 mg ethanedinitrile kg-1 artificial soil were applied through the septa into the jar.
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 5 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Executive summary:
Earthworm acute toxicity tests (artificial soil test) for Cyanogen (C2N2) using OECD 207 test method
This study was performed to determine the effects of ethanedinitrile on earthworm acute toxicity to comply with the requirements for an ecotoxicology study on plant protection products ‘Earthworm, Acute toxicity test’. The study was conducted according to the OECD Guideline for the testing of chemicals, Earthworm, Acute Toxicity Test: Guideline 207, the OECD Guideline for the testing of chemicals.Eisenia foetidaspecies was studied in artificial soil under laboratory conditions.
Seven rates at 0, 0.01, 0.1, 1.0, 10, 100 and 1,000 mg ethanedinitrile kg-1artificial soil were applied through the septa into the jar. The artificial soil was obtained by mixing 12.5% peat, 5% vermiculite, 20% top soil and 62.5% Florida sand. Soil pH was adjusted to 5.9 and moisture was maintained at 35%.
One litre capacity soil jars were induced with cyanogen in the lab according to the study protocol. All jars were capped with butyl rubber septa. All ethanedinitriletreatedjars were ventilated after 24 hours of application. The jars were opened after 12 hours of ventilation to count the number of live and dead earthworms. After counting, all materials were replaced in the jars. The jars were put under the light at room temperature 20 ± 2oC, soil moisture was adjusted when necessary, and mortality rate was recorded again on days 7 and 14. The average weight of live earthworms at the beginning and at the end of the experiment was recorded. Each treatment was replicated four times. The recoveries of earthworm up to 1 mg ethanedinitrilekg-1soil application levels were 100%.
The mortality rate was expressed and reported as median lethal concentration (LC50).
There was no evidence that ethanedinitrile affected earthworm average body weight at 1 mg C2N2Kg-1or lower rates. There were no distinct behavioural change or injury symptoms observed for the live earthworms after treatment.
All earthworms died in the 10 mg ethanedinitrilekg-1soil treatment level on day 0 of ethanedinitrile application. Over the 14-day observation period, no earthworm was affected by ethanedinitrile application at 1 mg ethanedinitrile kg-1soil or less. From the total number of mortalities recorded the LC100value is 10 mg ethanedinitrile kg-1soil. 24-hour LC50is 5 mg ethanedinitrile kg-1soil.
Reference
Description of key information
This study was performed to determine the effects of ethanedinitrile on earthworm acute toxicity to comply with the requirements for an ecotoxicology study on plant protection products ‘Earthworm, Acute toxicity test’. The study was conducted according to the OECD Guideline for the testing of chemicals, Earthworm, Acute Toxicity Test: Guideline 207, the OECD Guideline for the testing of chemicals.Eisenia foetidaspecies was studied in artificial soil under laboratory conditions.
One litre capacity soil jars were induced with cyanogen in the lab according to the study protocol. All jars were capped with butyl rubber septa. All ethanedinitrile treated jars were ventilated after 24 hours of application. The jars were opened after 12 hours of ventilation to count the number of live and dead earthworms. After counting, all materials were replaced in the jars. The jars were put under the light at room temperature 20 ± 2o C, soil moisture was adjusted when necessary, and mortality rate was recorded again on days 7 and 14. The average weight of live earthworms at the beginning and at the end of the experiment was recorded. Each treatment was replicated four times. The recoveries of earthworm up to 1 mg ethanedinitrile kg-1 soil application levels were 100%. The mortality rate was expressed and reported as median lethal concentration (LC50). There was no evidence that ethanedinitrile affected earthworm average body weight at 1 mg C2N2 Kg-1 or lower rates. There were no distinct behavioural change or injury symptoms observed for the live earthworms after treatment. All earthworms died in the 10 mg ethanedinitrile kg-1 soil treatment level on day 0 of ethanedinitrile application. Over the 14-day observation period, no earthworm was affected by ethanedinitrile application at 1 mg ethanedinitrile kg-1 soil or less. From the total number of mortalities recorded the LC100 value is 10 mg ethanedinitrile kg-1 soil. 24-hour LC50 is 5 mg ethanedinitrile kg-1 soil. Exposure of soil organisms is not expected as the fumigant is used for treatment of wood in enclosed spaces. The only emission of the active substance to the air will occur during ventilation of the fumigated wood. The substance will be dispatched in the air and will get quickly diluted due to its high volatility. The substance will stay in the air based on its physical and chemical properties and will not transfer to other environmental compartments such as soil and water as confirmed by models in the environmental fate part of dossier.Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 5 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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