Hexahydro-4,7-methano-1H-indenyl acrylate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Hexahydro-4,7-methano-1H-indenyl acrylate

EC Number:

235-697-2

EC Name:

Hexahydro-4,7-methano-1H-indenyl acrylate

Cas Number:

12542-30-2

Molecular formula:

C13 H16 O2

IUPAC Name:

2-Propenoic acid hexahydro-4,7-methano-1H-indenyl ester

Details on test material:

- Name of test material (as cited in study report): Dihydrodicyclopentadienylacrylate
- Physical state: liquid/colorless, clear
- Analytical purity: 97.7 corr. area-% as a sum of four single peaks with a molecular mass of 204. The main peak of these four peaks has 91.3 corr. area-%
- Lot/batch No.: 110019P040

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

-Test species and strain: Pseudokirchneriella subcapitata KORSHIKOV (SAG 61.81)
-Age: A stock algal culture is maintained continuously at the test facility. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 4 days at 21 – 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test (study day 0).
-Supplier: Collection of algal cultures in University of Göttingen /Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

23.2 - 23.5 °C

Nominal and measured concentrations:

0 (control), 0.32, 1.0, 3.2, 10 and 32 mg/L as nominal concentrations based on technical test substance mass without correction for purity
0 (control), 0.23, 0.78, 2.60, 8.26 and 26.6 mg/L as geometric mean measured concentrations based on technical test substance mass without correction for purity

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 250 mL) closed with glass plugs Glass flask closed with glass plugs (nominal volume 50mL); for additional uninoculated replicates 8 & 9 only.
- Type (delete if not applicable): closed
- Initial cells density: 0.3 x 104 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:
- Light intensity and quality: Average 4798 lux (within ± 15% variability) at a wave length of 400 - 700 nm

TEST SUBSTANCE PREPARATION
-The stock solution (35.5 mg/L) was prepared by pipetting test substance (165 µL based on the density) into 5L of test medium and stirring for about one day in a sealed flask. The test solutions were prepared at 111% of the nominal concentration to compensate for the dilution by the later addition of algal inoculum. The test concentrations were prepared from a dilution of a stock solution.
The dissolution of the test substance in water is slow. Therefore the stock solution was stirred for about one day. to ensure that the test substance was completely dissolved.
All test solutions were visibly clear following preparation.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2.99

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

1.58 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Algal Yield [mg/L]		Algal Growth Rate [mg/L]
EyC10 confidence limits 95%:	0.21 0.18 – 0.24	ErC10 confidence limits 95%:	1.58 1.17 – 2.14
EyC20 confidence limits 95%:	0.32 0.29 – 0.36	ErC20 confidence limits 95%:	1.97 1.63 – 2.38
EyC50 confidence limits 95%:	0.75 0.70 – 0.80	ErC50 confidence limits 95%:	2.99 2.68 – 3.34