Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 225-060-7 | CAS number: 4635-87-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
Oral: LD50 Males: 50 -300 mg/kg bw for rat
Dermal: LD50 Males: > 2000 mg/kg bw for rat
Inhalation: LC50 Males: > 0.5 - < 2.0 mg/L for rat
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Version / remarks:
- December 2001
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- Version / remarks:
- December 2002
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EC No 440/2008 Part B. Acute Oral Toxicity, Acute Toxic Class Method
- Version / remarks:
- May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Appendix to Director General Notification, No. 12-Nousan-8147. Agricultural Production Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan (JMAFF), November 2000, including the most recent revisions
- Version / remarks:
- November 2000
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
- Species:
- rat
- Strain:
- other: Wistar Han
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- based on previous test results (Ac. Oral tox. 1981 ROND) male rats were known to be significantly more sensitive than females
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 50 and 300 mg/kg bw
- No. of animals per sex per dose:
- 3 males
- Control animals:
- no
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 50 - <= 300 mg/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- male
- Dose descriptor:
- LD50 cut-off
- Effect level:
- 200 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 50 mg/kg bw: all 3 animals survived
300 mg/kg bw: all animals were found dead or sacrified in moribound condition between day 2 and 4 after treatment
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 200 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Version / remarks:
- September 2009
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- Version / remarks:
- August 1998
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EC No 440/2008, part B. Acute Toxicity (inhalation), May 2008, amended by COMMISSION REGULATION (EU) No 260/2014
- Version / remarks:
- 24 January 2014
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Appendix to Director General Notification, No. 12-Nousan-8147. Agricultural Production Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan (JMAFF)
- Version / remarks:
- November 2000
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- fixed concentration procedure
- Limit test:
- yes
- Species:
- rat
- Strain:
- other: Wistar Han
- Sex:
- male/female
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- 4.9.2. Test Atmosphere Concentration Sampling
A total of 6 and 8 representative samples were taken for determination of the actual
concentration during exposure at 2 and 0.5 mg/L, respectively. Samples were drawn from the
test atmosphere through a tube mounted in one of the free animal ports of the exposure
chamber. Sample volumes were measured by means of a dry gas meter (type G 1.6, Actaris
Meterfabriek B.V., Dordrecht, The Netherlands). The samples were drawn through series of
four impingers (2 mg/L) and four or two impingers (0.5 mg/L) filled with acetonitrile as
collection fluid. The amount of test item collected was measured analytically (analytical
method developed under test facility project number 20184667). Subsequently the timeweighted mean concentrations with the standard deviations were calculated.
At 2 mg/L, only the first two impinger samples were used for calculation as from sample 3
onwards the third and fourth impingers showed high values. Normally, these impinger
samples should be negligibly small and it was concluded that a mistake was made at sample 3
which contaminated the impingers at the subsequent measurements. Sample 3 was excluded
as the concentration was unrealistically high as no changes were made for the generation
settings. Inclusion of the sample would lead to an actual concentration exceeding the amount
of test item used (nominal concentration). - Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- 4 h
- Concentrations:
- Initially, 3-PENTENENITRILE was administered as a vapor by nose only inhalation for
4 hours to one group of five male and five female Wistar Han rats at a target concentration of
2 mg/L. Based on the results, the males were identified as most sensitive sex and therefore
five males were exposed at a target concentration of 0.5 mg/L. - No. of animals per sex per dose:
- 2 mg/L: 5 male and 5 female rats
0.5 mg/L: 5 male rats - Control animals:
- no
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 0.5 - <= 2 mg/L air (nominal)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- males 0.5 mg/L: 0
males 2.0 mg/L: 4
females 2.0 mg/L: 1 - Body weight:
- No abnormalities
- Gross pathology:
- At 2 mg/L, macroscopic post mortem examination revealed no test item related abnormalities
in any of the animals. At 0.5 mg/L, macroscopic examination revealed isolated black/brown
foci in the lungs of one male.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LC50
- Value:
- 2 000 mg/m³ air
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity: Fixed Dose Procedure)
- Version / remarks:
- 2017
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- fixed dose procedure
- Limit test:
- yes
- Species:
- rat
- Strain:
- other: Wistar Han
- Sex:
- female
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- The test item was applied in an area of approximately 10% of the total body
surface, i.e. approximately 18 cm² - Duration of exposure:
- 24 h
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 2000 mg/kg bw: 3 animals
- Control animals:
- not required
- Key result
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- no
- Gross pathology:
- No abnormalities were found at macroscopic post mortem examination of the animals.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 000 mg/kg bw
Additional information
Justification for classification or non-classification
Based on the LD50 males of 200 mg/kg bw, 3-Pentenenitrile is classified for acute oral toxicity as:
- Category 3 (H301, Toxic if swallowed) according to the CLP Regulation (1272/2008) as LD50 was found to be between 50 and 300 mg/kg bw.
Based on the LD50 males of > 2000 mg/kg bw 3-Pentenenitrile is not classified for acute dermal toxicity,
Based on the LC50 males of > 0.5 - < 2.0 mg/L, 3-Pentenenitrile is classified for acute inhalation toxicity as:
- Category 2 (H330 Fatal if inhaled) according to the CLP Regulation (1272/2008) as LD50 was found to be between 0.5 and 2 mg/L/4h.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)