Fatty acids, C8-18 and C18-unsatd., reaction products with diethanolamine and propylene oxide

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): About 10 g of a mixture of fine ground quartz sand and 550 mg of test substance were mixed with 740g wet artificial soil for each test container to form a test substance concentration of 1000 mg/kg dry weight.

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Zhuhai Hongming Earthworm Breeding park
- Age at test initiation (mean and range, SD): > 2 months
- Weight at test initiation (mean and range, SD): 552 - 571 mg

ACCLIMATION
- Acclimation period: 14d
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): no mortality observed

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

18.3 - 21.6 °C

pH:

6.84 - 6.89

Moisture:

25.55 - 29.65%

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1L glass beaker
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 554 lux (at the beginning) and 511 lux at the end of the test

EFFECT PARAMETERS MEASURED: Weight, behavior and mortality were recorded after 77 and 14 days exposure time.

Nominal and measured concentrations:

Nominal: 1000 mg/kg dw

Reference substance (positive control):

yes

Remarks:

Cloracetamide

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: none

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: 14d-LC50 was 19.71 mg/kg dry weight, and the 95% confidence Iimit was between 18.14 and 21.42 mg/kg dry weight, which was within the range of 10 - 100 mg/kg dry weight.

Validity criteria fulfilled:

yes

Conclusions:

the substance is not acutely harmful to earthworms.

Description of key information

LC50(14d) > 1000 mg/kg dw (nominal) for Eisenia foetida (OECD 207)

Additional information

One study investigating the short-term toxicity of Fatty acids, C8-18 and C18-unsatd., reaction products with diethanolamine and propylene oxide (CAS 1000817-22-0) to earthworms is available. The study was performed according to OECD guideline 207 under GLP conditions (Liu 2013). A test substance concentration of 1000 mg/kg dw soil was tested in a limit test using Eisenia foetida as test organism. No mortality was observed in control and treatment throughout the test duration of 14 days. Thus, the 14d-LC50 is determined to be > 1000 mg/kg dw soil based on nominal concentrations.