17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 Nov 2000 - 28 Dec 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one

EC Number:

259-701-7

EC Name:

17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one

Cas Number:

55542-27-3

Molecular formula:

C22H34O3

IUPAC Name:

17 beta-Hydroxy-17 alpha-(3-hydroxypropyl)-4-androsten-3-one

Details on test material:

- Name of test material (as cited in study report): Hydroxypropyltesto
- Analytical purity: 91.2%
- Lot/batch No.: 59012052

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

3.3 mg/L

Based on:

test mat.

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

> 10

Sampling time:

28 d

Any other information on results incl. tables

Table 2: Degradation ofthe test item and reference substance
Day		Degradation %
	Procedure control	Test item
1	51	0
4	76	1
7	79	3
11	86	0
14	85	13*
18	-	1
21	-	2
26	-	5
28	-	5
	*mean value of 21 % and 5 % degradation

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

In the present study Iess oxygen uptake by the test item was measured. The calculated results
do not exceed 10 % degradation. For this reason in the test for ready biodegradability the test
item seems to be not readily biodegradable.

Executive summary:

The ready biodegradation of Hydroxypropyltesto in an aerobic aqueous medium was tested by the Closed Bottle Test according to OECD guideline No. 301 D. Mineral medium containing a known concentration of the test item was inoculated with microorganisms from the eflluent of a treatment plant (domestic sewage) and incubated in the dark over a 28 day period. Degradation was followed by oxygen evolution twice a week. The amount of oxygen taken up by the microbial population during biodegradation of the test item was expressed as a percentage of the theoretical oxygen demand of the test item. Throughout the test no degradation of the test item was measured. The result is not due to inhibition of the inoculum. The result of a toxicity control showed that the concentration of test item tested has no inhibitory effect to the inoculum. The pass level for ready bio degradation (60 per cent degradation) was not reached. Thus in the test for ready biodegradability the test item seems to be not readily biodegradable.