Registration Dossier
Registration Dossier
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EC number: 700-225-0 | CAS number: 1160806-44-9
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethyl (2R,3R)-3,4-dibromo-2-methylbutanoate; ethyl (2R,3S)-3,4-dibromo-2-methylbutanoate; ethyl (2S,3R)-3,4-dibromo-2-methylbutanoate; ethyl (2S,3S)-3,4-dibromo-2-methylbutanoate
- EC Number:
- 700-225-0
- Cas Number:
- 1160806-44-9
- Molecular formula:
- C7 H12 Br2 O2
- IUPAC Name:
- ethyl (2R,3R)-3,4-dibromo-2-methylbutanoate; ethyl (2R,3S)-3,4-dibromo-2-methylbutanoate; ethyl (2S,3R)-3,4-dibromo-2-methylbutanoate; ethyl (2S,3S)-3,4-dibromo-2-methylbutanoate
- Details on test material:
- - Name of test material (as cited in study report): 2-Methyl-3,4-dibrombuttersaeureethylester
- Analytical purity: 98.1 w-%
- Lot/batch No.: 35684-12-5
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sludge taken from a waste water treatment plant of the city of Mannheim, Germany.
- Concentration of sludge: 4 mg/l - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 67.2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to ISO 14593
- Test temperature: 22 +/- 2°C
TEST SYSTEM
- Number of culture flasks/concentration: 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 20 - 30
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Interpretation of results:
- other: partly or moderately biodegradable
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