Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 211-219-8 | CAS number: 634-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: The study was not carried out following a guideline method nor under a quality assurance system. The method description lacks of detailed documentation and the raw data were not presented.
Data source
Reference
- Reference Type:
- publication
- Title:
- Genotoxicity of aniline derivatives in various short-term tests
- Author:
- Kugler-Steigmeier ME, Friederich U, Graf U, Lutz WK, Maier P, Schlatter C
- Year:
- 1 989
- Bibliographic source:
- Mutation Research, 1989, vol. 211, p. 279 - 289
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Somatic mutation and recombination test in Drosophila melanogaster: Larvae (72 h) transheterozygous for the mutations multiple wing hair (mwh, 3-0.0) and flare (flr, 3-38.8) were fed the test substance for 48 h. Spontaneous mutation frequency was determined from solvent controls. Induced spots on the wings were scored and subject to statistical analysis.
- GLP compliance:
- no
- Type of assay:
- somatic mutation and recombination test in Drosophila
Test material
- Reference substance name:
- 2,4,6-trichloroaniline
- EC Number:
- 211-219-8
- EC Name:
- 2,4,6-trichloroaniline
- Cas Number:
- 634-93-5
- Molecular formula:
- C6H4Cl3N
- IUPAC Name:
- 2,4,6-trichloroaniline
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): 2,4,6-TCA.
- Source: EGA Chemic, Stammheim (F.R.G.).
- Analytical purity: > 99 %, checked by gas chromatography.
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: trans-heterozygous for the mutations multiple wing hair (mwh, 3-0.0) and flare (flr, 3-38.8)
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 72 h old larvae.
- Diet (e.g. ad libitum): Drosophila Instant Medium (Formula 4- 24, Carolina Biological Supply Company, Burlington, NC, U.S.A.)
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- - Vehicle(s)/solvent(s) used: aqueous solution containing 3 % absolute ethanol and 1 % Tween 80.
- Justification for choice of solvent/vehicle: used to prepare Drosophila Instant Medium. - Duration of treatment / exposure:
- 48 hours.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
5 mM
Basis:
nominal in diet
spiked drosophila instant medium
- Control animals:
- yes, concurrent vehicle
Examinations
- Tissues and cell types examined:
- The larvae were treated, and the wings prepared and scored for induced spots according to Graf et al. (1984).
- Statistics:
- Statistical analysis of the data was carried out as described by Frei and Würgler (1988).
Results and discussion
Test results
- Sex:
- not specified
- Genotoxicity:
- positive
- Toxicity:
- not specified
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- The test item tended to induce mainly small spots, which suggests that the somatic mutations were induced late in the development
of the larvae, at a time point where only 1-2 more cell divisions took place. This phenomenon can be explained by the increasing activity of
the metabolizing enzymes in the course of the development of the larvae (Hallstrrm et al., 1983).
Any other information on results incl. tables
Table 1: Results
Number of wings | Spots per wing and statistical diagnoses | |||
Small single spot m=2.0 | Large single spots m=5.0 | Twin spots m=5.0 | Total spots m=2.0 | |
80 | 0.45 + | 0.08 - | 0.01 i | 0.54 + |
+ positive, - negative, w weak, i inconclusive. Probabifity levels α = ß = 0.05. One-sided statistical tests. Small single spots 1-2 cells, large single spots > 2 cells, m multiplication factor.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
From the absence of induced twin spots it can be concluded that, under the conditions of the somatic mutation assay in Drosophila the compounds did not exhibit strong recombinogenic activity and the somatic mutations were induced late in the development of the larvae.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.