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EC number: 258-053-2 | CAS number: 52628-03-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP/Guideline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-Propenoic acid, 2-methyl-, 2-hydroxyethyl ester, phosphate
- EC Number:
- 258-053-2
- EC Name:
- 2-Propenoic acid, 2-methyl-, 2-hydroxyethyl ester, phosphate
- Cas Number:
- 52628-03-2
- Molecular formula:
- C6 H10 O3 . x H3 O4 P
- IUPAC Name:
- 2-(methacryloyloxy)ethyl phosphate
- Test material form:
- other: semi-viscous liquid
- Details on test material:
- - Name of test material (as cited in study report): Reaction product of 2-Hydroxyethyl methacrylate (HEMA) with Polyphosphoric Acid (PPA)
- Physical state: clear very light yellow semi-viscous liquid
- Lot/batch No.: A022C73001
- Storage condition of test material: room temperature
Constituent 1
Test animals
- Species:
- human
- Details on test animals or test system and environmental conditions:
- ENVIRONMENTAL CONDITIONS
- Temperature (°C): The tissues exposed for 3 minutes were held at room temperature during dosing, while the tissues exposed for the 60 minutes were
incubated at standard culture conditions (37°) until the completion of the exposure time.
IN-LIFE DATES: From: To: 8/27/12 - 8/29/2012
Test system
- Type of coverage:
- not specified
- Preparation of test site:
- other: EpiDerm Skin Model
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): Fifty µL of the test substance were applied directly on the tissue so as to cover the upper surface.
- Concentration (if solution): The test substance was tested neat. - Duration of treatment / exposure:
- 3 minutes and 60 minutes
- Observation period:
- End of exposure
- Number of animals:
- n/a
- Details on study design:
- The test substance was added to a 1.0 mg/mL MTT (Sigma) solution in warm Dulbecco’s Modified Eagle’s Medium (DMEM) containing 2 mM L-glutamine (MTT Addition Medium) to assess its ability to directly reduce MTT. Approximately 50 µL of the test substance were added to 1 mL of the MTT solution, and the mixture was incubated at standard culture conditions for approximately one hour. A negative control, 50 µL of sterile, deionized water (Quality Biological), was tested concurrently. If the MTT solution color turned blue/purple, the test substance was presumed to have reduced the MTT.
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- other: other: cell viabiilty
- Value:
- ca. 23.9
- Remarks on result:
- other:
- Remarks:
- Basis: other: %. Time point: 3 minutes. (migrated information)
- Irritation / corrosion parameter:
- other: other: cell viability
- Value:
- ca. 1.4
- Remarks on result:
- other:
- Remarks:
- Basis: other: %. Time point: 60 minutes. (migrated information)
In vivo
- Irritant / corrosive response data:
- See Table 1.
Any other information on results incl. tables
Mean % Viability
Concentration | Exposure Time | % Viability | * Corrosive? | pH |
Neat | 3 minutes | 23.9 | Yes | 4.0 |
Neat | 60 minutes | 1.4 | Yes | 4.0 |
* - A material is considered corrosive if the percent viability is less than 50% at the 3 -minute exposure, or less than 15% at the 60 -minute exposure.
Applicant's summary and conclusion
- Interpretation of results:
- corrosive
- Remarks:
- Migrated information Criteria used for interpretation of results: other: UN GHS
- Conclusions:
- Based upon the results of this assay, the test substance, Reaction product of 2-Hydroxyethyl methacrylate (HEMA) with Polyphosphoric Acid (PPA), was predicted to be corrosive to skin according to the prediction model validated by ECVAM. Accordingly, the test substance, Reaction product of 2-Hydroxyethyl methacrylate (HEMA) with Polyphosphoric Acid (PPA), would be labeled corrosive to skin within the Globally Harmonized System.
- Executive summary:
The MatTek Corporation’s EpiDermTMreconstituted human epidermis model was used to assess the potential skin corrosivity of the test substance, Reaction product of 2-Hydroxyethyl methacrylate (HEMA) with Polyphosphoric Acid (PPA).The skin corrosivity potential of the testsubstancewas evaluated by measuring the relative cell viability in treated tissues after a 3- and 60-minute exposure to the testsubstance, following theprotocol that is consistent with the OECD Test Guideline 431 “In Vitro Skin Corrosion: Human Skin Model Test”. Based upon the results of this study, the test substance, Reaction product of 2-Hydroxyethyl methacrylate (HEMA) with Polyphosphoric Acid (PPA), is predicted to be a skin corrosive. Accordingly, the test substance,Reaction product of 2-Hydroxyethyl methacrylate (HEMA) with Polyphosphoric Acid (PPA), would be labeled corrosive to skin within the Globally Harmonized System.
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