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EC number: 212-091-6 | CAS number: 762-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Diethyl phosphonate
- EC Number:
- 212-091-6
- EC Name:
- Diethyl phosphonate
- Cas Number:
- 762-04-9
- Molecular formula:
- C4H11O3P
- IUPAC Name:
- diethyl phosphonate
- Test material form:
- other: clear liquid wotj weak odor
- Details on test material:
- content: 98.86 %
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source:
- Age at study initiation: 8-12 week
- Weight at study initiation:28-41 g
- Housing: males singly and females in groups of 3 mice
- Diet ad libitum
- Water ad libitum
- Acclimation period: at least one week
ENVIRONMENTAL CONDITIONS
- Temperature (°C):22
- Humidity (%):40-70
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- corn oil
- Details on exposure:
- singel intraperitoneal injections fo 1500 mg/kg bw
Sacrifice 16 hours, 24 and 48 hours post injection - Duration of treatment / exposure:
- once
- Frequency of treatment:
- once
- Post exposure period:
- up to 48 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0 and 1500 mg/kg bw
Basis:
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamid
Examinations
- Tissues and cell types examined:
- polychromatic erythrocytes in somatic tissue, the bone marrow
- Details of tissue and slide preparation:
- Schmid's method was uded to produce smears and stained
- Evaluation criteria:
- Normally 1000 polychromatic erythrocytes were counted per animal,
an alteration of the ration poychromatic to normochromatic erythrocytes may show that the test compoA test actually reaches the target
a test was considered positive if , at any of the intervals there was a relevant and significant increase in the number of poychromatic erythrocytes showing micronuclei in comparison to the negative control - Statistics:
- Wilcoxon*s non-parametric rank sum test. one-sided Chi--test, calculation of mean and standard deviation (1s)
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Executive summary:
Diethylphosphite was tested for clastogenic effects using the micronucleus test in accordance with OECD TG 474 and GLP. The known clastogen and cytostatic agend cyclophosphamide served as positive control.. The test animals received a single intraperitoneal injection of either diethyl phopsphite (1500 mg/kg bw) or cyclophosphamide, the femoral marrow of groups treated with diethyl phosphite was prepared 16, 24 and 48 hours after application.
The treated animals showed symptoms of toxicity after the administration , however, all animals survived until the end of the test.
There was an altered ratio between polychromatic and normochromatic erythrocytes.
No indications of a clastogenic effect of diethyl phopshphite were found. The positive control was functional..
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