Disulphur dichloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 16-1 to 28-2-1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable deviations (LD50 expressed in µl/kg bw . Analytical purity was not reported. No acclimatisation period. Individual weights were not reported)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

2000

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

LD50 expressed in µl/kg bw . Analytical purity was not reported. No acclimatisation period. Individual weights were not reported.

GLP compliance:

no

Remarks:

Study was conducted before GLP was mandatory

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Disulphur dichloride

EC Number:

233-036-2

EC Name:

Disulphur dichloride

Cas Number:

10025-67-9

Molecular formula:

Cl2S2

IUPAC Name:

dichlorodisulfane

Details on test material:

- Name of test material (as cited in study report): disulphur dichloride
- Analytical purity: no data
- Physical state: liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: 9 weeks (males), 14 weeks (females)
- Weight at study initiation: male rats weighed 166 g and female rats weighed 169 g at the beginning of the study
- Fasting period before study: rats were fastened 16 hrs before and 4 hrs after application of test substance
- Housing: Macrolon cage type III
- Diet (e.g. ad libitum): Altromin R1324 (Altromin GmbH, Lage, Germany) ad libitum (except during the fasting period).
- Water (e.g. ad libitum): tap water ad libitum



ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22± 1.5 °C
- Humidity (%): 60± 5%
- Photoperiod (hrs dark / hrs light): 12hrs dark/ 12 hrs light


IN-LIFE DATES: From: 16-1-1984 To: 28-2-1984

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

50 , 60, 70, 80, 100 , 500 µl/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: On the day of the application the animals were monitored several times. On the following 14-day observation period the animals were examinated for mortality and clinical signs. The description, the onset, the duration and the intesity of the clinical symptome were recorded and the dead animals eliminated.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, grosspathology.

Statistics:

The calculation of the LD50 with confidence limits for p less than 0.05, was determined according to Rosiello et al., 1977, J. Tox Environ Health 3, 797.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

132 mg/kg bw

95% CL:

>= 69.7 - <= 89.4

Remarks on result:

other: Slope=3.28

Clinical signs:

other: Rats which died during assay or sacrificed after the assay showed the following signs: poor general condition, scrubby fur, prone/lateral position, narcosis, growth delay.

Gross pathology:

Rats which died during assay or sacrificed after the assay showed the following signs: after high doses changes of the stomach mucosa and corrosion. Liver and spleen were partial black-brown coloured.

Any other information on results incl. tables

Intensity , appearance of symptoms and death are described
as follows:

group        dose       number       time of death
           [µl/ml]    [m/f/total]   
 1           50         0/0/10            -
 2           60         2/10/10        4 -8 days
 3           70         4/10/10        3 -6 days
 4           80         6/10/10      8 hours - 7 days
 5          100         7/10/10     30 min - 6 days
 6          500        10/10/10     30 min - 8 days

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: other: EU-GHS

Executive summary:

Loeser (Bayer AG), 1984

Acute oral toxicological examinations were conducted with disulphur dichloride on male and female Wistar rats, according to OECD guideline 401 with deviations (LD50 expressed in µL/ kg bw instead of mg/kg bw. Analytical purity was not reported. Animals were not subjected to acclimation period. Indivual weights were recorded just at the beginning and at the end of the application.Changes in weight were not reported).

Six different single oral doses of the test substance (50, 60, 70, 80, 100 and 500 µl/kg bw) were administered via gavage to 5 male and 5 female rats. The post-exposure observation period was 14 days.

The calculated LD50 resulted to be 78 µL/kg body weight (132 mg/kg bw; slope = 3.28), for male and female rats.


The following poisoning symptoms were observed: poor general condition, scrubby fur, prone position/lateral position, narcosis, delay in growth.

On the basis of the results of this test, disulphur dichloride can be classified as:

EU: T, R25: Toxic if swallowed

OECD GHS: Acute oral toxicity-Category 3.Warning H301, Toxic if swallowed.