Registration Dossier
Registration Dossier
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EC number: 269-927-8 | CAS number: 68391-08-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The test substance is not toxic in concentrations <1000 mg/L to activated sludge of a municipal
sewage treatment plant.
Key value for chemical safety assessment
Additional information
A Respiration lnhibition Test with activated sludge according to OECD Guideline No. 209 and GLP principles was performed for 3 hrs with the test substance. The test system was activated sludge of the municipal treatment plant. The test method was static. Nominal concentrations of the test item were as follows:100, 180, 320, 580, and 1000 mg/L. The respiration rates of control, reference and test item were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. The inhibition ranged from 2 to 9 %. The EC20, EC50 and EC80 values could not be calculated in the tested concentration range. In order to check the activity of the test system and the test conditions a reference test is carried out once every 7 days with copper-(II)-sulphate-pentahydrate as reference item and the reference toxicity is determined. The EC50 value for the reference item was 104 mg/L.
Validity criteria of the test guideline were fulfilled.
The test substance is not toxic in concentrations < 1000 mg/L to activated sludge of a municipal sewage treatment plant.
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