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EC number: 266-442-3 | CAS number: 66669-53-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 11/93
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Please find the Justification attached to Chapter 13.2.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Verlängerter Toxizitätstest beim Zebrabärbling Brachydanio rerio (Schwellenkonzentration der letalen und anderer Wirkungen; NOEC, min. 14 Tage) Umweltbundesamt Berlin, Stand 01.02.1984
- Deviations:
- yes
- Remarks:
- , Cited in the study report: conditions of temperature, but not relevant for test result
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- yes
- Remarks:
- , Cited in the study report: conditions of temperature, but not relevant for test result
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - applikations method: direct weighing
- solution water and dilution water: synthetic fresh water according to ISO - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio rerio; Zebra danio
- Source: West-Aquarium (Bad Lauterberg; Germany)
- Length: 2.5 - 3.5 cm
- Mortality during pre-exposure to synthetic fresh water according to ISO: < 2%
- Medical pre-treatment: no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- NA (The fishes were scarified after 14 days of exposure and then the length and bodyweight were determined).
- Hardness:
- 12.8 - 15.4°dH
- Test temperature:
- ca. 21°C (+/-2°C)
- pH:
- 7.8 mg/L - 9.6 mg/L
- Dissolved oxygen:
- 7.8 mg/L - 9.6 mg/L
- Salinity:
- according to ISO
- Nominal and measured concentrations:
- Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-12; 30-34; 100-109; 309-337; 994-1086 - Details on test conditions:
- TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Test medium: 5 L, aerated
- Vehicle: no
- No. of organisms per vessel and concentration: 10 - Reference substance (positive control):
- not required
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Details on results:
- A LC50 (96h) of > 1042 mg/L was derived from the available prolonged toxicity study in fish over 14 days (result: LC50 (21d) > 1042 mg/L).
No mortality was recorded for this prolonged toxicity study in 10 fishes (species: zebra danio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls. - Validity criteria fulfilled:
- yes
- Conclusions:
- A LC50 (96h) of > 1042 mg/L was concluded from the results of the prolonged toxicity study in fish.
- Executive summary:
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid is proposed.
In aqueous media, tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphono-butane-1,2,4-tricarboxylic acid dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of 2-phosphonobutane-1,2,4-tricarboxylic acid and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.
Therefore a read-across between tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphonobutane-1,2,4-tricarboxylic acid is justified.
A LC50(96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish ( species: zebra danio) over 14 days. Thus, the LC50(96h) is higher than the concentration of the limit test (100 mg/L). No further testing (in respect of classification and labelling etc.) is required.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11/93
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Verlängerter Toxizitätstest beim Zebrabärbling Brachydanio rerio (Schwellenkonzentration der letalen und anderer Wirkungen; NOEC, min. 14 Tage) Umweltbundesamt Berlin, Stand 01.02.1984
- Deviations:
- yes
- Remarks:
- , Cited in the study report: conditions of temperature, but not relevant for test result
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- yes
- Remarks:
- , Cited in the study report: conditions of temperature, but not relevant for test result
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - applikations method: direct weighing
- solution water and dilution water: synthetic fresh water according to ISO - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio rerio; Zebra danio
- Source: West-Aquarium (Bad Lauterberg; Germany)
- Length: 2.5 - 3.5 cm
- Mortality during pre-exposure to synthetic fresh water according to ISO: < 2%
- Medical pre-treatment: no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- NA (The fishes were scarified after 14 days of exposure and then the length and bodyweight were determined).
- Hardness:
- 12.8 - 15.4°dH
- Test temperature:
- ca. 21°C (+/-2°C)
- pH:
- 7.8 mg/L - 9.6 mg/L
- Dissolved oxygen:
- 7.8 mg/L - 9.6 mg/L
- Salinity:
- according to ISO
- Nominal and measured concentrations:
- Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-12; 30-34; 100-109; 309-337; 994-1086 - Details on test conditions:
- TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Test medium: 5 L, aerated
- Vehicle: no
- No. of organisms per vessel and concentration: 10 - Reference substance (positive control):
- not required
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Details on results:
- A LC50 (96h) of > 1042 mg/L was derived from the available prolonged toxicity study in fish over 14 days (result: LC50 (21d) > 1042 mg/L).
No mortality was recorded for this prolonged toxicity study in 10 fishes (species: zebra danio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls. - Validity criteria fulfilled:
- yes
- Conclusions:
- A LC50 (96h) of > 1042 mg/L was concluded from the results of the prolonged toxicity study in fish.
- Executive summary:
A LC50(96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish ( species: zebra danio) over 14 days. Thus, the LC50(96h) is higher than the concentration of the limit test (100 mg/L). No further testing (in respect of classification and labelling etc.) is required.
Referenceopen allclose all
Description of key information
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate , results of the parent acid, 2-phosphonobutane-1,2,4-tricarboxylic acid are taken into account for this endpoint.
A LC50 (96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 1 042 mg/L
Additional information
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid is proposed.
In aqueous media, tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphono-butane-1,2,4-tricarboxylic acid dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of 2-phosphonobutane-1,2,4-tricarboxylic acid and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.
Therefore, a read-across between tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphonobutane-1,2,4-tricarboxylic acid is justified.
Prolonged toxicity of 2-phosphonobutane-1,2,4-tricarboxylic acid to fish (Danio rerio) has been tested under semistatic conditions in accordance to OECD Guideline 204 for an exposure period of 14 days. No mortality was recorded for this prolonged toxicity study in 10 fishes (species: Danio rerio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls. A NOEC of >/= 1042 mg/l is obtained in this test. The result is interpreted as 14 d-LC50 of > 1042 mg/l referring to measured concentrations, as the test concentrations were confirmed by analytical monitoring using ion chromatography.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to fish (Danio rerio).
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