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EC number: 266-577-8 | CAS number: 67109-27-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 March 1994 to 31 March 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 11
- Expiration date of the lot/batch: December 1998
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under storage conditions: December 1998
- Stability under test conditions: < 1 g/l in water - Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: The nominal concentrations tested were 4.6, 10.0, 21.0, 46.0 and 100 mg test substance/l and a control.
Concentrations in excess of 100 mg/l have not been tested. - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Test organism:
The study was performed with young daphnids (at the start of the test 6 - 2 4 hours old) of a clone of the species Daphnia magna Straus. The clone is bred in the laboratories of RCC under standardized conditions. The cultivation of the parental daphnids was performed in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test.
- Experimental conditions:
In each test concentration and control 20 daphnids were tested, divided into four groups of five animals, each group in 125 ml test medium in a
glass-beaker (250 ml). The daphnids were not fed during the test period.
The test was performed in an air-conditioned room.
Water temperature: 21.1 - 21.2 °C during test period.
Light: 16 hours per day, approximately 200 - 600 Lux.
The daphnids have not been fed, and the test media have not been airated during the test period. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.5 mmol/l (= 250 mg/l) as CaCO3
- Test temperature:
- 21.1-21.2 °C
- pH:
- Control: 8.1 (start, 0h) and 8.0 (end, 48 h)
At 100 mg/l: 7.9 (start, 0h) and 8.0 (end, 48 h) - Dissolved oxygen:
- Control: 8.8 mg/l (start, 0h)
At 100 mg/l: 9.0 mg/l (start, 0h)
After 48 h:
8.7-9.0 mg/l - Salinity:
- Ratio of Ca : Mg = 4 : 1; Na : K =.10 : 1
- Nominal and measured concentrations:
- Nominal concentration: 100.0, 46.0, 21.0, 10.0 and 4.6 mg/l
Measured concentrations: - Details on test conditions:
- TEST MEDIUM / WATER PARAMETERS:
Test water: reconstituted water: in deionized water with a conductivity lower than 0.1 µS/cm (Milli-Q water) analytical grade salts were dissolved to following nominal concentrations, and the water was aerated to oxygen saturation:
CaCl2 x 2H20: 2.0 mmol/l (= 294 mg/l)
MgS04 x 7H20: 0.5 mmol/l (= 123 mg/l)
NaHC03: 0.75 mmol/l (= 65 mg/l)
KCl: 0.075 mmol/l (= 5.8 mg/l) - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 75.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 46.0-123.1 mg/l
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Test results:
24-h EC 50: > 100 mg test substance/l
24-h EC 0: > 100 mg test substance/l
48-hour EC 50:75.3 mg test substance/l
(95 % conf. limits: 46.0 - 123.1 mg test substance/l)
48-hour EC 0: 4.6 mg test substance/l
48-hour NOEC: 4.6 mg test substance/l
48-hour EC 100: > 100 mg test substance/l
48-hour EC 90: 369 mg test substance/l
(95 % conf. limits : 126 - 1080 mg test substance/l) - Conclusions:
- The 24 and 48 h EC50 of FAT 20028/D is > 100 mg/l and 75.3 mg/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l), respectively.
- Executive summary:
The 48-h toxicity test was carried out to evaluate the influence of the test substance on the mobility respectively survival of daphnids. Therefore, juvenile daphnids are exposed to the test substance for 48 h, added to the water at a range of concentrations. Under otherwise identical test conditions, and an adequate range of test substance concentrations, different concentrations of the test substance result in different percentages of daphnids being no longer capable of swimming at the end of the test or being dead. The acute toxicity of the test substance FAT 20'028/D to Daphnia magna Straus was determined in a 48-h static test according to the Commission Directive 92/69/EEC, Annex Part C.2, dated December 29, 1992 and the OECD Guideline for Testing of Chemicals, Section 2, No. 202, Part I, adopted April 04, 1984. The test was performed in compliance with Good Laboratory Practice Regulations. The nominal concentrations tested were 4.6, 10.0, 21.0, 46.0 and 100 mg test substance/1 and a control. Concentrations in excess of 100 mg test substance/l have not been tested (in accordance with the Commission Directive 92/69/EEC).
The biological test results:
24 -h EC 50: > 100 mg/l
24-h EC 0: > 100 mg/l
48-h EC 50:75.3 mg test substance/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l)
48-h EC 0: 4.6 mg test substance/l
48-h NOEC: 4.6 mg test substance/l
48-h EC 100: > 100 mg test substance/l
48-h EC 90: 369 mg test substance/l (95 % conf. limits : 126 - 1080 mg test substance/l)
The analytically determined test substance concentrations in the test media varied in the range from 108.8 % to 121.0 % of the nominal concentrations. During the test period the test substance concentrations slightly decreased to 91.3 % to 110.7 % of the nominal values, obviously due to a sedimentation of the suspended test substance. However, in spite of this sedimentation the mean measured test substance concentrations still amounted from 102.2 % to 115.3 % of the nominal test concentrations. Therefore, all reported biological results are related to the nominal concentrations of the test substance.
Reference
Description of key information
The 48 h EC50 and NOEC of Acid Red 211 in an acute toxicity to Daphnia magna Straus was 75.3 mg/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l) and 4.6 mg/l, respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 75.3 mg/L
Additional information
The 48-h toxicity test was carried out to evaluate the influence of the test substance on the mobility respectively survival of daphnids. Therefore, juvenile Daphnia magna are exposed to the test substance for 48 h, added to the water at a range of concentrations. Under otherwise identical test conditions, and an adequate range of test substance concentrations, different concentrations of the test substance result in different percentages of daphnids being no longer capable of swimming at the end of the test or being dead.
The acute toxicity of the test substance FAT 20'028/D to Daphnia magna Straus was determined in a 48-h static test according to the Commission Directive 92/69/EEC, Annex Part C.2, dated December 29, 1992 and the OECD Guideline for Testing of Chemicals, Section 2, No. 202, Part I, adopted April 04, 1984. The test was performed in compliance with Good Laboratory Practice Regulations.
The nominal concentrations tested were 4.6, 10.0, 21.0, 46.0 and 100 mg test substance/l and a control. Concentrations in excess of 100 mg test substance/l have not been tested (in accordance with the Commission Directive 92/69/EEC).
The biological test results:
24 -h EC50: > 100 mg/l
24-h EC0: > 100 mg/l
48-h EC50:75.3 mg test substance/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l)
48-h EC0: 4.6 mg test substance/l
48-h NOEC: 4.6 mg test substance/l
48-h EC100: > 100 mg test substance/l
48-h EC90: 369 mg test substance/l (95 % conf. limits : 126 - 1080 mg test substance/l)
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