Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-987-8 | CAS number: 112-58-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 July to 26 August 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- Adopted January 21, 2000
- Deviations:
- yes
- Remarks:
- The temperature increased above the temperature range recommended by OECD guideline 216 to a high of 36.3 ℃ overnight from 27 to 28 July 2022.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on preparation and application of test substrate:
- A 100-mg/mL primary stock solution was prepared by dissolving 1.0143 g (1.0001 g active ingredient) of dihexyl ether in 10.0 mL of acetone. This 100-mg/mL stock solution was used to dose the dihexyl ether-treated soils.
Seven 10-g batches of quartz sand were prepared for the 1.0, 3.0, 9.0, 27, 81, and 243 mg/kg test substance, and control treatment groups. The nitrification sand was dosed with 0.1, 0.3, 0.9, and 2.7 mL of the 10-mg/mL dihexyl ether stock solution to achieve 1.0, 3.0, 9.0, and 27 mg/kg concentrations, respectively. To achieve the 81 and 243 mg/kg concentrations, the nitrification sand was dosed with 0.81 and 2.43 mL of the 100-mg/mL dihexyl ether stock solution, respectively. The solvent was allowed to evaporate off before the dosed sand was mixed into the soil. Seven 1.0-kg (dry weight equivalent) batches of soil were weighed out into beakers. Starting with the control, each batch of soil was dosed and mixed as follows. Approximately half of the soil batch was transferred to a bowl of a Hobart mixer. An appropriate amount of the test substance was added across the soil surface while mixing the soil using the food mixer. The remaining half of the soil batch was then added to the bowl. The fortified soil was mechanically mixed for approximately 10 minutes at low speed. During this mechanical mixing, the soil was amended with 5.0 g of lucerne meal (i.e., alfalfa). Also during this mechanical mixing, a volume of purified reagent water was added to the soil in order to bring the moisture content to 45 ± 5% of the soil’s maximum water holding capacity. After dosing and mixing, each batch of soil was divided equally into three replicate test samples in 1-L glass bottle test vessels. - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- The temperature was monitored continuously with a minimum/maximum thermometer, and ranged from 19 to 22 °C.
- Moisture:
- The soil moisture content was maintained at approximately 45 ± 5% of the maximum water holding capacity throughout the test. The moisture content of the soil samples was checked and adjusted, if necessary, once a week.
- Organic carbon content (% dry weight):
- 0.64
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Smithers
- Test container (type, material, size): 1-L glass bottles
- Amount of soil: 1 kg
- No. of replicates per concentration: 3
- No. of replicates per control: 3
- No. of replicates per vehicle control: 0
SOIL INCUBATION
- Method: bulk / series of individual subsamples: 3 replicates - The 1-L glass bottle test vessels were sealed with perforated parafilm, which allowed for air exchange and avoided loss in moisture. The samples were incubated at a temperature of 20 °C in a dark environmental climate chamber. The temperature was monitored continuously with a minimum/maximum thermometer, and ranged from 19 to 22 °C. The soil moisture content was maintained at approximately 45 ± 5% of the maximum water holding capacity throughout the test. The moisture content of the soil samples was checked and adjusted, if necessary, once a week.
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): Offenbach, Rheinland-Pfalz, Germany, “rechts der Landauer Str.”, No. 826/7
- Treatments with pesticides or fertilizers: None used within the last 5 years
- Depth of sampling: Collected from the top 20 cm of the field
- Soil texture
- % sand: 63
- % silt: 28
- % clay: 9
- Soil taxonomic classification: Sandy Loam
- Soil classification system: USDA
- pH (in water): 6.4
- Maximum water holding capacity (g/100 g dry soil): 24.3
- Cation exchange capacity (meq/100 g): 6.1
- Pretreatment of soil: passed through a 2-mm sieve
- Storage (condition, duration): stored refrigerated (approximately 4 °C) and in the dark
- Initial microbial biomass as % of total organic C: 1.43
DETAILS OF PREINCUBATION OF SOIL (if any): Before use, the soil was transferred to an environmental chamber set to maintain a temperature of 20 ± 2°C and allowed to equilibrate to incubation conditions for 2 to 28 days (8 days, actual).
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Nitrate concentration at 0 and 28 d.
VEHICLE CONTROL PERFORMED: no - acetone evaporated off before use - Nominal and measured concentrations:
- 1.0, 3.0, 9.0, 27, 81, and 243 mg a.i./kg dry weight soil
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 3.3 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: difference was due to a stimulatory effect of the nitrate formation and not an inhibitory effect
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- 46.6 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: difference was due to a stimulatory effect of the nitrate formation and not an inhibitory effect
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 3 752 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: extrapolated value since higher than highest concentration
- Details on results:
- No inhibitory effects were observed in the study, only a stimulatory effect of the nitrate formation
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results of the study indicated that dihexyl ether had a stimulatory effect on the nitrate formation rate at the concentrations tested, resulting in an EC10 value of approximately 3.3 mg/kg. The EC25 and EC50 were determined to be 46.6 and 3752 mg/kg, respectively. The EC50 value was extrapolated since it was higher than the highest concentration tested.
- Executive summary:
Dihexyl ether exposure did not result in inhibitory effects to nitrate rate formation in an OECD 216. Statistically significant stimulatory effects were observed at the two highest concentrations of 81 and 243 mg/kg.
Reference
Description of key information
Dihexyl ether was not inhibitory to the nitrate formation rate in an OECD 216 guideline study. Statistically significant stimulatory effects were observed at the two highest concetrations of 81 and 243 mg/kg.
Key value for chemical safety assessment
- Short-term EC50 for soil microorganisms:
- 3 752 mg/kg soil dw
- Long-term EC10 or NOEC for soil microorganisms:
- 3.3 mg/kg soil dw
Additional information
Dihexyl ether exposure did not result in inhibitory effects to nitrate rate formation in an OECD 216. Statistically significant stimulatory effects were observed at the two highest concentrations of 81 and 243 mg/kg.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.