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Diss Factsheets
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EC number: 204-299-0 | CAS number: 119-13-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09/05/1995 - 22/06/1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according to international standard (OECD guideline 201) performed under GLP, validity criteria met.
Data source
Reference
- Reference Type:
- other: published parts of study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-benzopyran-6-ol
- EC Number:
- 233-466-0
- EC Name:
- 3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-benzopyran-6-ol
- Cas Number:
- 10191-41-0
- Molecular formula:
- C29H50O2
- IUPAC Name:
- 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-3,4-dihydro-2H-1-benzopyran-6-ol
- Test material form:
- liquid: viscous
Constituent 1
- Specific details on test material used for the study:
- No test surrogate or analogue material was used.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal concentrations were 0, 10, 18, 32, 58 and 100 mg test substance/l
- Sampling method: composite samples (about 150 ml per concentration and control) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the test vessels. Samples of test solutions were taken immediately before exposure and after 72 hours exposure and stock solution (=highest test concentration) was taken 24 hours before and a dark sample after 72h of exposure.
- Sample storage conditions before analysis: stored at 18 - 22°C
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 200.8 mg test substance were mixed in 800 ml reconstituted water including 4 mg Tween 80 by polytron and then made up to 2000 ml with reconstituted water
- Controls: yes, reconstituted water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): polyoxyethylen-sorbitan-mono-oleat (Tween 80)
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 2 mg/l
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: Pseudokirchneriella subcapitata 61.81 SAG (=Selenastrum capricornutum ATCC 22662)
- Source (laboratory, culture collection): Collection of algae cultures - Pflanzenphysiologisches Institut University, Göttingen, Germany
- Method of cultivation: according to OECD guideline 201
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test conditions
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 30 mg CaO/l
- Test temperature:
- 24 +/- 1 °C
- pH:
- 8.0 - 8.2 and 9.1-10.0 at the start and end of exposure, respectively.
- Nominal and measured concentrations:
- Nominal concentrations: 10, 18, 32, 58 and 100 mg/l
Measured concentrations (start): 7.45, 12.50, 19.35, 22.50 and 31.05 mg/l
Measured concentrations (end): 5.30, 8.65, 13.65, 15.10 and 20.55 mg/l
Effect concentrations (based on actual mean concentrations): 6.4, 10.6, 16.5, 18.8 and 25.8 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): closed (cotton stopper)
- Material, size, headspace, fill volume: glass, 100 ml (total volume 125 ml), 50 ml medium per test vessel
- Aeration: No
- Initial cells density: 9600 cells/ml
- Control end cells density: 1578000 cells/ml
- No. of organisms per vessel: No data
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Conductivity: 160 uS
- Culture medium different from test medium: No
- Intervals of water quality measurement: pH measured after 0 and 72 hours
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: cold white fluorescent light at 120 u E/m^2 +/- 20% (approximately 8000 lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]: TOA cell counter
- Chlorophyll measurement: No
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 25.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 25.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 25.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 25.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): No
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- No reference substance was used.
- Reported statistics and error estimates:
- A modified Dennet Multiple Comparison Test was used for calculation of the NOEC. No error estimates available or 95% confidence intervals are
available.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72h EC50 for d,l-a-tocopherol towards Selenastrum capricornutum for growth rate was found to be >25.8 mg/l, 72h NOEC for growth rate
was found to be 25.8 mg/l. - Executive summary:
Toxicty of d,l-a-tocopherol towards Selenastrum capricornutum was determined according to OECD guideline 201 under GLP conditions. The 72h EC50 for growth rate was found to be >25.8 mg/l, 72h NOEC for growth rate was found to be 25.8 mg/l.
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