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EC number: 203-224-9 | CAS number: 104-66-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Date of study initiation: September 13, 1988; Date of animal purchase: October 20, 1988; Date of administration: October 27, 1988; Date of autopsy: November 10, 1988; Date of Final report development: January 13, 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guideline compliant study
- Justification for data waiving:
- other:
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- other: Crj:CD (SD) SPF
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories Japan Inc.
- Age at study initiation: 4 weeks old when were purchased. Subsequently, animals with good growth and general conditions were chosen at the age of 5 weeks to use in this study.
- Weight at study initiation: The mean body weight (the range of body weight) of males and females on administration was 126 g in male (122 - 129 g) and 103 g in female (100 - 106 g), respectively.
- Fasting period before study: Animals were fasted and given only water from 5pm thte day before administration to 3 hr after administration
- Housing: 2 or 3 animals were housed in a stainless metal cage (W 276 x D 426 x H 200 mm) separately by sex.
- Diet (e.g. ad libitum): Animals were fed ad libitum with pellet (Lab M R Stock, Nosan Corporation, Japan) and water (tapping water sterilized by filtration with 1-μ cartridge filter and UV irradiation).
- Water (e.g. ad libitum): Animals were fed ad libitum with pellet (Lab M R Stock, Nosan Corporation, Japan) and water (tapping water sterilized by filtration with 1-μ cartridge filter and UV irradiation).
- Acclimation period: habituated in the test conditions for 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 24±2
- Humidity (%): 55±10
- Air changes (per hr): more than 10 times/hr (all fresh air) of ventilation
- Photoperiod (hrs dark / hrs light): 12-hr light and 12-hr dark cycle (light on: 6 AM, light off: 6 PM) - Route of administration:
- oral: gavage
- Vehicle:
- other: methyl cellulose
- Details on oral exposure:
- Preparation and administration procedures of test substance: The test substance were prepared with solvent 1.0 w/v% solution of methylcellulose (100cP, lot No.: AWL 3082, Wako Pure Chemical Industries) using an agate mortar and centrifugal ball mill to make suspension at 25 w/v%, the highest concentration physically available. The volume of administration was 20 mL/kg.
The test substance was orally administered by gastric gavage. Animals were fasted and given only water from 5 PM the day before administration to 3 hr after administration. - Doses:
- The volume of administration was 20 mL/kg (see above).
- No. of animals per sex per dose:
- 5
- Control animals:
- not specified
- Details on study design:
- Observation items: The observation period was 14 days after administration and general symptoms and death/survival were confirmed at least three times from immediately after administration to 6 hr (Administration Day 0) and at least once a day from the next day to the completion of observation. Body weight was measured immediately before administration, and 1, 3, 7 and 14 days after administration. On the final day of observation period (14 days after administration), all animals were sacrificed by ether anesthesia and autopsied.
- Statistics:
- No details provided in report
- Preliminary study:
- Preliminary dose-finding study: Based on the results of a preliminary dose-finding study, no death was found in both male and female animals even at a dose of 5,000 mg/kg, which was almost the upper limit physically and technically available. Consequently, 1, 2-diphenoxyethane was considered to be extremely low toxic. Therefore, the dose for male and female animals was determined 5,000 mg/kg. Of each 12 male and female animals, 5 each with good general conditions after habituation were randomly chosen to use in the study.
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no effects at highest dose tested
- Mortality:
- Mortality and lethal dose: No death was observed in male and female animals for 14 days of observation period. Therefore, the minimum lethal dose for males and females was 5,000 mg/kg or more.
See Table 1 of attached report - Clinical signs:
- other: General symptoms: No change in general conditions was found in both male and female animals from immediately after administration and no toxic symptom caused by the test substance was found. See Table 2 and Appendices 1-2 of attached report
- Gross pathology:
- Autopsy findings: No gross abnormal finding was observed in organs of male and female animals.
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute oral LD50 1, 2-diphenoxyethane to rats was > 5000 mg/kg bw
- Executive summary:
An acute oral toxicity study of 1, 2-diphenoxyethane was conducted in rats and the results are as follows.
1. Dose: Male and female: 5,000 mg/kg
2. Minimum lethal dose: Male: >5,000 mg/kg Female: >5,000 mg/kg
3. General symptoms: No toxic symptom was observed in male and female rats.
4. Changes in body weight: No effect on body weight gain was found in male and female rats.
Reference
No death was observed in male and female animals and the minimum lethal dose was 5,000 mg/kg or more.
In addition, general symptoms, body weight gain and autopsy findings indicated no toxic effect of the test substance.
In conclusion, acute toxicity of 1, 2-diphenoxyethane to rats was considered to be minimal.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 5 000 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
Reference
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Oral toxicity: No death was observed in male and female animals and the minimum lethal dose was 5,000 mg/kg or more. In conclusion, acute toxicity of 1, 2-diphenoxyethane to rats was considered to be minimal.
Justification for selection of acute toxicity – oral endpoint
Only available study
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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