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EC number: 248-654-8 | CAS number: 27776-01-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 2012-06-29 - 2012-12-19
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The GLP study was conducted according to an internationally accepted guideline. All study parameters are given in detail. Nevertheless, according to the ECHA's practical guide 6: "How to report read-across and categories" the maximum for read-cross is 2.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.17 (Mutagenicity - In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Phenyl-tolyl-ethane
- IUPAC Name:
- Phenyl-tolyl-ethane
- Reference substance name:
- 40766-30-1
- Cas Number:
- 40766-30-1
- IUPAC Name:
- 40766-30-1
- Test material form:
- other: liquid
- Details on test material:
- Name PTE
Batch no. Trailer
Appearance Clear Liquid
Composition 87.5% phenyl-tolyl-ethane (PTE)
10.8% 1,1-diphenylethane (DPE)
CAS No. 40766-30-1
EINECS-No. not stated
Molecular formula C15H16
Molecular weight 196 g/mol
Purity 87.5% PTE (GC)
Homogeneity homogeneous
Vapour pressure 0.0025 hPa at 20°C
Stability unknown
Solubility H2O: < 0.1g/L; acetone; CH3CN: > 1 g/L;
EtOH, DMSO: unknown
Storage Room Temperature: (20 ± 5°C)
Constituent 1
Constituent 2
Method
- Target gene:
- Autosomal thymidine kinase (TK) locus of heterozygous L5178Y/TK+/- cells
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Additional strain / cell type characteristics:
- other: deficient in thymidine kinase
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver enzyme S9 fraction
- Test concentrations with justification for top dose:
- In the first experiment, the following concentrations (real) of the test item were used:
963 µg/mL, 481.5 µg/mL, 240.8 µg/mL, 120.4 µg/mL, 60.2 µg/mL, 30.1 µg/mL, 15.0 µg/mL and 7.5 µg/mL.
In the second experiment, the following concentrations (real) of the test item were used:
488.8 µg/mL, 244.4 µg/mL, 122.2 µg/mL, 61.1 µg/mL, 30.5 µg/mL, 15.3 µg/mL, 7.6 µg/mL and 3.8 µg/mL. - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Details on test system and experimental conditions:
- For mycoplasma contamination screened stocks of cells are stored in liquid nitrogen in the cell bank to allow a continuous stock of cells, which guarantees similar parameters of the experiment and reproducible characteristics of the cells.
The L5178Y cells are thawed 120 hours prior to treatment and cultivated in RPMI 1640 complete culture medium (with 5% horse serum) in cell culture flasks (75 cm2)and incu-bated at 37.0 ± 1.5 °C in a humidified atmosphere with 5% CO2. - Evaluation criteria:
- The test item is considered to have mutagenic effects if:
the induced mutation frequency reproducibly exceeds a threshold of 126 colonies per 106 cells above the corresponding solvent control.
the relative increase of the mutation frequency shows a dose relationship.
A mutagenic response is considered to be reproducible if it occurs in both parallel cul-tures.
Results of test groups are generally rejected if the relative total growth is less than 10% of the solvent control.
The biological relevance of the results is always considered first. Appropriate statistical methods are used as an aid in evaluating the test results. However, the results of statisti-cal testing were assessed with respect to dose-response relationship. Reproducibility and historical data was also taken into consideration.
Statistical significance was confirmed by means of the non-parametric χ2 test. However, both biological and statistical significance were considered together.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Treatment |
Conc. (µg/ml) |
Relative Total Growth Culture A |
Mutants per 106 cells Culture A |
Relative Total Growth Culture B |
Mutants per 106 cells Culture B |
Relative Total Growth Mean |
Mutants per 106 cells Mean |
Exp. I with metabolic activation (S9), exposition time 4 hours |
|||||||
Negative control |
-- |
-- |
173 |
-- |
103 |
-- |
138 |
Solvent control DMSO |
5 µL/mL |
-- |
124.5 |
-- |
140 |
-- |
132.25 |
Solvent control 0.9% NaCl |
5 µL/mL |
-- |
140 |
-- |
142.5 |
-- |
141.3 |
Positive control CPA |
4.5 |
27.6% |
551 |
61.3% |
570.5 |
44.5% |
560.75 |
Test Item |
963 |
0.1% |
-- |
1.0% |
360 |
0.5% |
180 |
Test Item |
482 |
0.0% |
-- |
0.0% |
-- |
0.0% |
-- |
Test Item |
241 |
5.0% |
255 |
6.0% |
230 |
5.5% |
242.5 |
Test Item |
120 |
51.6% |
140 |
68.9% |
102.5 |
60.2% |
121.25 |
Test Item |
60 |
70.0% |
147.5 |
74.9% |
156 |
72.5% |
151.75 |
Test Item |
30 |
90.4% |
125.5 |
86.1% |
168 |
88.2% |
146.75 |
Test Item |
15 |
78.7% |
161.5 |
90.8% |
160 |
84.8% |
160.75 |
Test Item |
7.5 |
122.9% |
109.5 |
107.4% |
158.5 |
115.1% |
134 |
Exp. I without metabolic activation, exposition time 4 hours |
|||||||
Negative control |
-- |
-- |
249.5 |
-- |
236 |
-- |
242.75 |
Solvent control DMSO |
5 µL/mL |
-- |
229.5 |
-- |
160.5 |
-- |
195 |
Positive control MMS |
19.5 |
40.0% |
581 |
54.3% |
592.5 |
47.1% |
586.75 |
Test Item |
963 |
0.0% |
-- |
0.1% |
-- |
0.0% |
-- |
Test Item |
482 |
0.0% |
-- |
0.0% |
-- |
0.0% |
-- |
Test Item |
241 |
1.1% |
581 |
1.4% |
894 |
1.2% |
737.5 |
Test Item |
120 |
44.5% |
232 |
64.5% |
270 |
54.5% |
251 |
Test Item |
60 |
109.0% |
141 |
97.6% |
244.5 |
103.3% |
192.75 |
Test Item |
30 |
86.1% |
172 |
107.9% |
264.5 |
97.0% |
218.25 |
Test Item |
15 |
107.3% |
214 |
94.0% |
315.5 |
100.6% |
264.75 |
Test Item |
7.5 |
134.4% |
129.5 |
119.6% |
212 |
127.0% |
170.75 |
Values above threshold (see below) are given in bold characters.
Threshold for mutagenic effects: number of mutant colonies per 106cells of the respective solvent control plus 126.
Threshold negative contr. (medium) with metabolic activation: 264 mutants/106cells
Threshold negative contr. (medium) without metabolic activation: 368.75 mutants/106cells
Threshold solvent control NaCl 0.9% with metabolic activation: 267.3 mutants/106cells
Threshold solvent control DMSO with metabolic activation:256.3 mutants/106cells
Threshold solvent control DMSO without metabolic activation: 321 mutants/106cells
Treatment |
Conc. (µg/ml) |
Relative Total Growth Culture A |
Mutants per 106 cells Culture A |
Relative Total Growth Culture B |
Mutants per 106 cells Culture B |
Relative Total Growth Mean |
Mutants per 106 cells Mean |
Exp. II with metabolic activation (S9), exposition time 4 hours |
|||||||
Negative control |
-- |
-- |
173.5 |
-- |
139.5 |
-- |
156.5 |
Solvent control DMSO |
5 µL/mL |
-- |
138 |
-- |
101 |
-- |
119.5 |
Solvent control 0.9% NaCl |
5 µL/mL |
-- |
156.5 |
-- |
135.5 |
-- |
146 |
Positive control CPA |
4.5 |
62.3% |
536.5 |
45.1% |
659 |
53.7% |
597.75 |
Test Item |
488 |
0.0% |
-- |
0.0% |
-- |
0.0% |
-- |
Test Item |
244 |
8.9% |
188.5 |
8.9% |
269 |
8.9% |
228.75 |
Test Item |
122 |
66.3% |
201.5 |
71.0% |
193.5 |
68.7% |
197.5 |
Test Item |
61 |
73.9% |
210.5 |
62.4% |
269.5 |
68.2% |
240 |
Test Item |
31 |
73.6% |
176.5 |
86.4% |
201 |
80.0% |
188.75 |
Test Item |
15 |
85.2% |
187.5 |
106.7% |
239.5 |
96.0% |
213.5 |
Test Item |
7.6 |
69.7% |
235 |
89.0% |
245 |
79.3% |
240 |
Test Item |
3.8 |
75.5% |
231 |
98.6% |
233 |
87.1% |
232 |
Exp. II without metabolic activation, exposition time 24 hours |
|||||||
Negative control |
-- |
-- |
128.5 |
-- |
139 |
-- |
133.75 |
Solvent control DMSO |
5 µL/mL |
-- |
152 |
-- |
125.5 |
-- |
138.75 |
Positive control MMS |
19.5 |
14.7% |
1341.5 |
12.1% |
1363 |
13.4% |
1352.25 |
Test Item |
488 |
0.0% |
-- |
0.0% |
-- |
0.0% |
-- |
Test Item |
244 |
0.7% |
239.5 |
0.4% |
520.5 |
0.5% |
380 |
Test Item |
122 |
73.9% |
231 |
59.3% |
282.5 |
66.6% |
256.75 |
Test Item |
61 |
113.6% |
195 |
86.6% |
245.5 |
100.1% |
220.5 |
Test Item |
31 |
94.2% |
226 |
97.4% |
231 |
95.8% |
228.5 |
Test Item |
15 |
96.3% |
208.5 |
91.0% |
202 |
93.7% |
205.25 |
Test Item |
7.6 |
91.5% |
165.5 |
79.8% |
189 |
85.6% |
177.25 |
Test Item |
3.8 |
92.0% |
216.5 |
109.7% |
243 |
100.8% |
229.75 |
Values above threshold (see below) are given in bold characters.
Threshold for mutagenic effects: number of mutant colonies per 106cells of the respective solvent control plus 126.
Threshold negative contr. (medium) with metabolic activation: 382.5 mutants/106cells
Threshold negative contr. (medium) without metabolic activation: 259.75 mutants/106cells
Threshold solvent control NaCl 0.9% with metabolic activation: 272 mutants/106cells
Threshold solvent control DMSO with metabolic activation:245.5 mutants/106cells
Threshold solvent control DMSO without metabolic activation: 264.75 mutants/106cells
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test item did not induce mutations in the mouse lymphoma thymidine kinase locus assay using the cell line L5178Y in the absence and presence of metabolic activation. Therefore, PTE is considered to be non-mutagenic under the conditions of the mouse lymphoma assay. - Executive summary:
This study was performed to investigate the potential of PTEto induce mutations at the mouse lymphoma thymidine kinase locus using the cell line L5178Y.
The assay was performed in two independent experiments, using two parallel cultures each (replicates). The first main experiment was performed with and without liver microsomal activation and a treatment period of 4 h. The second experiment was performed using a 4 h treatment period with metabolic activation and a 24 h treatment period without metabolic activation.
MMS (19.5 µg/mL) and CPA (4.5 µg/mL) were used as positive controls and showed a distinct increase in induced total mutant colonies and an increase of the relative quantity of small versus large induced colonies.In the first experiment, eight concentrations (ranging from 963 to 7.5 µg/mL) of the test item soluble in DMSO were used and tested with and without metabolic activation (4 hours incubation).Precipitation of the test item was not observed. In non-toxic concentrations, no increase in mutation frequency could be detected.
In the second experiment, eight concentrations (ranging from 488.8 to 3.8 µg/mL) of the test item soluble in DMSO were used and tested with (4 hours incubation) and without metabolic (22 hours incubation) activation.Precipitation of the test item was not observed. In non-toxic concentrations, no increase in mutation frequency could be detected in the second experiment, either.
In the first and in the second experiment, in the treatment without metabolic activation, a minor dose-response relationship was found. But as all mutant frequencies in non-toxic concentrations lay below the threshold (solvent control plus 126 colonies/106cells), this effect was considered as not relevant for the classification of the test item.
An increase in mutant colony numbers was observed in the both experiments in the treatment without metabolic activation: the concentration 245 µg/mL (nominal) showed an increase of the mutant frequency. This concentration showed a clear cytotoxic effect towards the cells, though (relative total growth was 1.2% resp. 0.5 %); therefore, the increase of the mututant colonies was considered as artefact.
In conclusion, it can be stated that under the experimental conditions reported, the test item did not induce mutations in the mouse lymphoma thymidine kinase locus assay using the cell line L5178Y in the absence and the presence of metabolic activation.
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