Dodecanedioic acid, 1,12-bis[2-[4-(4,6-diphenyl-1,3,5-triazin-2-yl)-3-hydroxyphenoxy]ethyl] ester

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 November 2013 - 23 March 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study conducted in a GLP compliant lab according to recognised regulatory guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: the definitive test employed water accommodated fractions with nominal loading rates of 4.27, 9.39, 20.7, 45.5 and 100 mg/L as T-1620L.
- Sampling method: No samples taken as study based on WAF's
- Sample storage conditions before analysis: No samples taken as study based on WAF'S.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: water accomodated fractions (WAF,s ) prepared
- Eluate: Elendt 4
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None
- Concentration of vehicle in test medium (stock solution and final test solution): N/a
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:Daphnia magna (Straus)
- Source: cultured in-house and were obtained from a strain originating from the National Institute for Applied Chemical Research (IRCHA), France
- Age at study initiation (mean and range, SD): less than 24h old
- Feeding during test: unicellular green algae, Pseudokirchneriella subcapitata, to provide nominally 0.1 to 0.2 mg carbon per daphnid, per day, except during the initial three days when a slightly lower ration was given

ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): n/a
- Type and amount of food: unicellular green algae, Pseudokirchneriella subcapitata, to provide nominally 0.1 to 0.2 mg carbon per daphnid, per day, except during the initial three days when a slightly lower ration was given
- Feeding frequency: daily
- Health during acclimation (any mortality observed):Good - no mortality observed

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

The total hardness of the batches of Elendt M4 medium used were 246 and 260 mg/L as CaCO3, respectively

Test temperature:

Continuous monitoring of an additional vessel containing dilution medium = 20.3 to 21.0°C

pH:

The alkalinity of the batches of Elendt M4 medium used were 39.6 and 52.0 mg/L as CaCO3, respectively

Dissolved oxygen:

Air saturation value (100% ASV = 9.09 mgO2/L at 20ºC)

Salinity:

N/a

Nominal and measured concentrations:

the definitive test employed water accommodated fractions with nominal loading rates of 4.27, 9.39, 20.7, 45.5 and 100 mg/L as T-1620L.

Details on test conditions:

TEST SYSTEM
- Test vessel:120 mL glass jars
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume:n/a
- Aeration:
- Renewal rate of test solution (frequency/flow rate): renewed every 24h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates): 4

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: None
- Observations on body length and weight: N/a
- Other biological observations: no significant immobilisation observed
- Mortality of control: 0
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None noted
- Effect concentrations exceeding solubility of substance in test medium: None

Results with reference substance (positive control):

- Results with reference substance valid? yes
- 48-hour EC50 - 0.934 mg/L

Validity criteria fulfilled:

yes

Conclusions:

After 48 hours of exposure of Daphnia magna to T-1620L as water accommodated fractions (WAF) with a maximum nominal loading rate of 100 mg/L, no significant immobilisation was observed. Therefore the EL50 value could not be calculated but must be >100 mg/L (nominal loading rate), whilst the “no observed effect loading rate” (NOELR) was 100 mg/L, the highest nominal loading rate tested.

Executive summary:

After 48 hours of exposure of Daphnia magna to T-1620L as water accommodated fractions (WAF) with a maximum nominal loading rate of 100 mg/L, no significant immobilisation was observed. Therefore the EL₅₀value could not be calculated but must be >100 mg/L (nominal loading rate), whilst the “no observed effect loading rate” (NOELR) was 100 mg/L, the highest nominal loading rate tested.

Description of key information

48 hour toxicity study:

EC₅₀(immobilisation): > 100 mg/L

NOELR (immobilisation): 100 mg/L