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Diss Factsheets
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EC number: 231-445-0 | CAS number: 7554-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Information published in peer reviewed literature, limitations in design and/or reporting but otherwise adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 989
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Surface soil samples were treated with test substance and incubated, after which the percentage inhibition of nitrification was calculated.
- GLP compliance:
- no
Test material
- Reference substance name:
- 4-methylpyrazole
- EC Number:
- 231-445-0
- EC Name:
- 4-methylpyrazole
- Cas Number:
- 7554-65-6
- Molecular formula:
- C4H6N2
- IUPAC Name:
- 4-methyl-1H-pyrazole
- Details on test material:
- - Name of test material (as cited in study report): 4-Methylpyrazole
Constituent 1
Sampling and analysis
- Details on sampling:
- Soil samples were placed in 25 x 200 mm test tubes and treated with 6 mL water containing 4 mg nitrogen as (NH4)2SO4 and 0, 0.01, 0.10, or
0.50 µmol test compound/g soil. The test tubes were then placed unsealed in an incubator maintained at 25 °C. The amount of water lost from
the soil samples during the incubation was measured gravimetrically at 5-day intervals and was replaced if it exceeded 1 mL. After 21 days, of test tubes were removed from the incubator, and their triplicate contents were analyzed for NH4+-N, NO2--N, and NO3--N as described by Bremner and Keeney (1966).
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- The soils used were surface (0-15 cm) samples of Iowa soils selected to obtain a range in pH (6.1-8.1), texture (9%-60% sand, 12%-49% silt, 28%-42% clay), organic-C content (1.2%-4.2%), and CaCO3 equivalent (0%-4,1%). Before use, each sample was air-dried and crushed to pass through a 2-mm screen.
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 21 d
Test conditions
- Nominal and measured concentrations:
- 0.01; 0.1; 0.5 µmol/g
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 2 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: based on visual interpolation of test data (Webster soil)
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 4 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: based on visual interpolation of test data (Harps soil)
- Reported statistics and error estimates:
- The amount of (NO3- +NO2-)-N produced during the incubation was calculated from the results of analyses for (NO3- +NO2- )-N before and after the incubation, and the percentage inhibition of nitrification by the test compound was calculated from (C-T)/Cx 100, where T is the amount of (NO3-+NO2)-N produced in the soil sample treated with the test compound and C is the amount of (NO3 +NO2 )-N produced by the control sample (no test compound added). None of the incubated soil samples contained more than 1 µg NO 2-N per g of soil, and no NH4+N was detected after incubation for 21 days of the (NH4)2SO4-amended soil samples that had not been treated with a test compound.
Any other information on results incl. tables
Note from the reviewer: the article only provides inhibition of nitrification % per concentration. EC50 Effect values were interpolated via log normal interpolation and there after linear regression to establish the EC50
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.