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EC number: 618-829-7 | CAS number: 922165-31-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 March 2011 - 05 May 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- : Inadvertently, 20 daphnids per conc were exposed to the control and the highest test concentration during the range-finding test. Evaluation: The test was suitable for gaining information about the range of concentrations to be used in the final test.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- (2008)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea)) (1996)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Neosolue-Aqulio
- IUPAC Name:
- Neosolue-Aqulio
- Reference substance name:
- 1,4-bis[2-(2-ethoxyethoxy)ethyl] cyclohexane-1,4-dicarboxylate
- EC Number:
- 618-829-7
- Cas Number:
- 922165-31-9
- Molecular formula:
- C20H36O8
- IUPAC Name:
- 1,4-bis[2-(2-ethoxyethoxy)ethyl] cyclohexane-1,4-dicarboxylate
- Details on test material:
- - Name of test material (as cited in study report): Neosolue-Aqulio
- Description/Physical state: Clear colourless to very pale yellow liquid
- Molecular formula: C20H36O8
- Molecular weight: 404.5
- Analytical purity: 99.5% (GC)
- Lot/batch No.: TVI-714
- Expiration date of the lot/batch: 07 October 2012 (Retest date)
- Storage condition of test material: At room temperature in the dark
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/l and control (0 mg/l) at t=0 and t=48 h
- Sampling method: 2 ml from the approximate centre of the test vessels
- Sample storage conditions before analysis: Samples were stored in a freezer until analysis
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- method: Test substance was stirred in medium at a nominal concentration of 100 mg/l applying 15 minutes of magnetic stirring to accelerate the dissolution. The lower test concentrations for the range-finding test were prepared by subsequent dilutions of the 100 mg/l concentration in test medium. The final test solutions were all clear and colourless.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Source: In-house laboratory culture with a known history.
- Age at study initiation: < 24 hours
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- Between 19.6 and 20.3°
- pH:
- Between 7.9 and 8.0
- Dissolved oxygen:
- Between 9.0 and 9.2 mg/l
- Nominal and measured concentrations:
- Nominal: loading rate 0 and 100 mg/l
Measured: 100 mg/l (start) and 99 mg/l (end) in the pooled solutions prepared at 100 mg/l.
Analysis of the samples taken during the limit test showed that measured concentrations were in agreement with nominal at the start (100%) and remained stable during the 48-hour exposure period (99% of nominal at the end). Given these results, effect parameters were based on nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass, containing 80 ml of test solution
- Type (delete if not applicable): open
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Introduction of daphnids: Within 15 minutes after preparation of the test solutions
- other: no feeding
TEST MEDIUM / WATER PARAMETERS
- Test medium: Adjusted ISO medium
- Source/preparation of dilution water: tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
- the following chemicals (analytical grade) are dissolved in the purified tap water:
CaCl2.2H2O 211.5 mg/l
MgSO4.7H2O 88.8 mg/l
NaHCO3 46.7 mg/l
KCl 4.2 mg/l
- Culture medium different from test medium: yes (M7 medium is used for breeding)
OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: 16 hours daily
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mobility
- mortality
TEST CONCENTRATIONS
Range finding study:
- Test concentrations: 0, 0.1, 1, 10, 100 mg/l
- Results used to determine the conditions for the definitive study: EC50
Final test:
- Test concentrations: 0, 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobility was observed at both the control and the limit concentration during the test period.
Analysis of the samples taken during the limit test showed that measured concentrations were in agreement with nominal at the start (100%) and remained stable during the 48-hour exposure period (99% of nominal at the end). Given these results, effect parameters were based on nominal concentrations. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
The 24h-EC50 was 0.68 mg/l with a 95% confidence interval between 0.61 and 0.78 mg/l.
The 48h-EC50 was 0.55 mg/l with a 95% confidence interval between 0.50 and 0.63 mg/l. - Reported statistics and error estimates:
- No EC50 could be calculated because the test substance proved to be non-toxic (EC50 > maximum concentration tested).
Any other information on results incl. tables
In a range-finding test, no (significant) immobility was observed at any of the test concentrations (0.1, 1, 10, 100 mg/L; nominal concentration was 100 mg/L, lower concentrations are dilutions thereof) and the control during the test period (48 h). Based on the range-finding test results, the expected EC50was above a nominal concentration of 100 mg/l.
The results of measurement of pH and oxygen concentrations (mg/l) remained within the limits prescribed by the protocol (pH: 6.0-8.5, not varying by more than 1.5 unit; oxygen: >=3 mg/l at the end of the test).
The temperature continuously measured in a temperature control vessel varied between 19.6 and 20.3°C during the test, and complied with the requirements as laid down in the protocol (18-22°C, constant within 2°C).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the described test, Neosolue-Aqulio did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). Hence, the 48h-EC50 was >100 mg/l.
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