N-(2,5-dimethoxyphenyl)acetamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed and documented OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(2,5-dimethoxyphenyl)acetamide

EC Number:

222-423-1

EC Name:

N-(2,5-dimethoxyphenyl)acetamide

Cas Number:

3467-59-2

Molecular formula:

C10H13NO3

IUPAC Name:

N-(2,5-dimethoxyphenyl)acetamide

Constituent 2

Reference substance name:

3467-59-

IUPAC Name:

3467-59-

Details on test material:

purity: 99%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The test substance was wetted with ethanol (0.1 ml/L), then water was added and the mixture was homogenized with an ultra-turrax. This mixture was tipped into the testing container and stirred. The test substance was not dissolved completely; there were residues at the wall and on the bottom.
The concentration refers to the total amount of test substance added into the test vessels.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Brachydanio rerio
- Strain: Hamilton-Buchanan
- Source: West Aquarium GmbH, 3422 Bad lauterberg
- Age at study initiation : about two months
- Length at study initiation: 2.4 - 3.2 cm
- Feeding during test: twice a day ad libitum
- Food type: Tetra Min, Tetra Werke Melle

ACCLIMATION
- Acclimation period: 14 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.2 - 22.4 °C

pH:

7.3 - 8.2

Dissolved oxygen:

6.7 - 9.8 mg O2/L

Nominal and measured concentrations:

only nominal concentrations were considered

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

350 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

other: LC90

Effect conc.:

710 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

596 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 526-675

Details on results:

- Other biological observations: at 1000 mg/L decreased breathing frequency
- Other adverse effects control: 350 -710 mg/L abnormal swimming behaviour, reduced activity, increased breathing activity
- Abnormal responses: Dead fish were dark coloured

Results with reference substance (positive control):

no reference substance used

Reported statistics and error estimates:

LD50 was calculated from the available test results by "Probitanalyse" (according to Lindner and Weber with confidential interval acc. Fiedler)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on a LC50 = 596 mg/L it is not required to classify Acetchinonbase TTRS for acute toxicity to fish

Executive summary:

Acetchinonbase TTRS was tested for acute toxicity against Brachydanio rerio acc. OECD 203 in concentrations of 350, 500, 710 and 1000 mg/L for 96 hours.With 500 - 1000 mg/L symptoms and letality occured, with 350 mg/L only symptoms but no lethality occured.

Based on these resultsa LC50 value could be estiamated by Probit Analysis . LC50 = 596 mg/L (526 - 675 mg/L).