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EC number: 237-415-3 | CAS number: 13776-88-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Septermber - December 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein- Westfalen
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal loadings of 1, 10 and 100 mg/L
- Sampling method: Samples were taken directly after test solution preparation and after 72 hours.
- Sample storage conditions before analysis: Exactly 20 mL of aqueous samples were transferred into disposable 50 mL polyethylene vials
(Sarstedt, Nuembrecht, Germany), and acidified to a target concentration of 1 % nitric acid (200
μL, dilution of sample negligible). Al concentrations were determined using ICP-OES (see
A.3.8.1) within three days. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared according to the Water Accommodated Fraction (WAF) approach according to the OECD
guidance documents No. 23 and 29. The test concentrations were prepared separately by adding the required amount of test substance to 1 litre sterile growth medium. After stiirring for 24 hours at 20°C the insoluble parts were separated by filtration (Sarstedt Bottle Top Filter, Filtropur
BT50, 0.2 μm PES (polyethylensulfone) membrane). - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Pseudokirchneriella subcapitata, Chlorophycea, Chlorophyta; 61.81 SAG
- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologisches Institut of the University at Göttingen, Albrecht von Haller Institut, Untere Klarspüle 2, D-37073 Göttingen, Catalog No 61.81 SAG.
- Age of inoculum (at test initiation):
- Method of cultivation: The stock cultures are maintained fulfilling the criteria of the OECD guideline (culture medium recommended by Bringmann
und Kühn (1980)). Prior to testing a pre-culture was established in standard OECD growth medium to obtain exponentially-growing algae for the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.0 – 22.1 °C
- pH:
- 7.53 - 7.84 (test solutions)
7.86 - 8.01 (control) - Nominal and measured concentrations:
- Nominal test item loadings: Control, 1.1, 9.9 and 99.8 mg/L, equivalent to theoretical nominal aluminium loading Control, 0.127, 1.14 and 11.5 mg Al/L.
Mean measured concentrations of aluminium are 14.7, 32.9 and 115 μg Al/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks (250 mL) covered with silicone sponge caps
- Fill volume: 100 mL
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic OECD standard medium according to OECD 201
OTHER TEST CONDITIONS
- Photoperiod: continous illumination
- Light intensity and quality: 98.3 to 103.7 μmol/m²s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: the cell concentrations was determined after 24, 48 and 72 hours using an electronic particle counter (CASY 1 Model TT,
OMNI Life Science GmbH & Co. KG, Bremen, Germany).
TEST CONCENTRATIONS
- Range finding study:yes
- Test concentrations: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: no inhibition up to 100 mg/L (nominal) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 115 other: µg Al /L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: Aluminium concentrations
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 115 other: µg Al /L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: Aluminium concentrations
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none - Results with reference substance (positive control):
- The sensitivity of the test organism is checked in regular intervals. In the latest test an ErC50 value of 3.78 mg/L was determined
- Reported statistics and error estimates:
- The NOEC values for growth rate and yield were determined by the Dunnett’s test using the computer program ToxRat® Professional
- Validity criteria fulfilled:
- yes
Reference
Inhibition of growth rate and yield compared to controls after 72 hours
Treatment, [μg Al/L] |
Growth rate |
Yield |
||||
Growth rate [1/d] |
% Inhibition |
Yield [cells x 104/mL] |
% Inhibition |
|||
Control |
1.630 |
- |
132.075 |
- |
||
14.7 |
1.602 |
1.7 (-) |
122.025 |
7.6 (-) |
||
32.9 |
1.603 |
1.6 (-) |
122.845 |
7.0 (-) |
||
115 |
1.619 |
0.6 (-) |
128.225 |
2.9 (-) |
||
(-) not significantly different from controls, Dunnett’s test, significance level 0.05, one-sided smaller.
Description of key information
EC50 (72 h) > 100 mg/L (nominal)
NOEC (72 h) ≥ 100 mg/L (nominal)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
The acute toxicity of aluminium metaphosphate (CAS 13776-88-0) to algae was investigated in a study by Wenzel (2015). This test was conducted according to OECD 201 under GLP conditions. Since the test item is of low water solubility, the nominal test concentrations were individually prepared using the Water Accommodated Fraction (WAF) approach according to the OECD guidance documents No. 23 and 29 to obtain the maximal solubility of the test item under the test conditions.
72 h EC50 value of >100 mg/L (nominal) and NOEC of ≥100 mg/L (nominal) have been determined for the effects of the structural related aluminium metaphosphate (CAS 13776-88-0) on the growth of Pseudokirchneriella subcapitata (Wenzel, 2014).
Thus the substance is considered not acutely toxic to algae.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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