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EC number: 220-813-6 | CAS number: 2905-62-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Non mutagenic with and without metabolic activation, bacterial reverse mutation assay, OECD 471 and EU Method B.13/14, Verspeek-Rip (2015)
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
The mutagenic activity of the test material was evaluated in a bacterial reverse mutation assay conducted in accordance with the standardised guidelines OECD 471 and EU Method B.13/14 under GLP conditions. The study was assigned a reliability score of 1 in line with the principles for assessing data quality as defined by Klimisch et al. (1997).
Salmonella typhimurium strains TA1535, TA1537, TA100 and TA98 and Escherichia coli strain WP2uvrA were exposed to the test material in two independent experiments; a direct plate assay was performed followed by a pre-incubation assay, both in the absence and presence of metabolic activation (S9-mix).
In a dose range finding study, the test material was initially tested up to concentrations of 5000 μg/plate in acetone in the strains TA100 and WP2uvrA in the direct plate assay. The test material precipitated on the plates at dose levels of 512 μg/plate and above. Toxicity was observed in both tester strains.
In the first mutation experiment, the test material was tested up to concentrations of 1600 μg/plate in the strains TA1535, TA1537 and TA98. The test material precipitated on the plates at dose levels of 512 μg/plate and above. Toxicity was observed in all tester strains.
In the second mutation experiment, the test material was initially tested up to concentrations of 1600 μg/plate in the strains TA100 and WP2uvrA in the pre-incubation assay. The test material precipitated on the plates at the dose level of 1600 μg/plate. Toxicity was observed in both tester strains.
In the main experiment, the test material was tested in the strains TA1535, TA1537 and TA98 up to concentrations of 890 and 512 μg/plate in the absence and presence of S9-mix, respectively. The test material did not precipitate on the plates at the top dose of 890 μg/plate. Toxicity was observed in all tester strains, except for the tester strain TA98 in the presence of S9-mix.
The test material did not induce a significant dose-related increase in the number of revertant colonies in any of the strains both in the absence and presence of S9-metabolic activation. These results were confirmed in an independently repeated experiment.
In this study, acceptable responses were obtained for the vehicle and positive controls, indicating that the test conditions were adequate and that the metabolic activation system functioned properly.
Under the conditions of the study, the test material was determined to be non-mutagenic in both the presence and absence of metabolic activation.
Justification for classification or non-classification
In accordance with the criteria for classification as defined in Annex I, Regulation (EC) No. 1272/2008, the substance does not require classification with respect to genetic toxicity.
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