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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP but without analytical determintion of the test item

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted 1984-04-04
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-amino-2,5-dimethoxy-N-phenylbenzenesulphonamide
EC Number:
257-821-4
EC Name:
4-amino-2,5-dimethoxy-N-phenylbenzenesulphonamide
Cas Number:
52298-44-9
Molecular formula:
C14H16N2O4S
IUPAC Name:
4-amino-2,5-dimethoxy-N-phenylbenzene-1-sulfonamide

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium 3422 Bad lauterberg
- Length at study initiation (length definition, mean, range and SD): 2.9 +-0.23 cm
- Feeding during test:
- Food type: Tetra Min; Tetra Werke, Melle
- Amount: ad libitum
- Frequency: twice a day

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions :same as test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21 - 23 °C
pH:
7.3 - 7.9
Dissolved oxygen:
4.7 - 9.3 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
2 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: LC20
Effect conc.:
3 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: LC70
Effect conc.:
5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC50 for fish toxicity of the test item is 4370 mg/L
Executive summary:

With the test item a test for fish toxicity was performed acc.to OECD 203. Concentrations of 2500mg/l, 3500 mg/l, 5000 mg/l and 10000 mg/l were exposed to 10 fish per experiment.

The highest tested concentration without lethality was 2500 mg/L

The lowest tested concentration with 100% lethality was 10000mg/L.

The LC50 was determined with a probit analysis (method Lindner and Weber) as 4370 mg/L (3750 - 5500 mg/L)