Registration Dossier
Registration Dossier
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EC number: 203-118-2 | CAS number: 103-50-4
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Dibenzyl ether: Local Lymph node assay
- Author:
- Betts CJ, Parker G, Dearman RJ
- Year:
- 2 007
- Bibliographic source:
- RIFM Database
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Dibenzyl ether
- EC Number:
- 203-118-2
- EC Name:
- Dibenzyl ether
- Cas Number:
- 103-50-4
- Molecular formula:
- C14H14O
- IUPAC Name:
- [(benzyloxy)methyl]benzene
- Details on test material:
- purity: 98.52%
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- other: CBA/ca/Ola/Hsd
- Sex:
- female
Study design: in vivo (LLNA)
- Vehicle:
- other: 1:3 ethanol:diethyl phthalate
- Concentration:
- 25 µl of 1.0, 2.5, 5.0, 10 or 25% (w/v).
- No. of animals per dose:
- 24 female mice.
Results and discussion
In vivo (LLNA)
Results
- Parameter:
- SI
- Remarks on result:
- other: The EC3 value was calculated to be 6.3% (1575 µg/cm²)
Any other information on results incl. tables
Under the conditions of the study, dibenzyl ether is a weak sensitiser.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Executive summary:
In a LLNA a dose of ca. 25 µl of 25 µl of 1.0, 2.5, 5.0, 10 or 25% (w/v) dibenzyl ether in 1:3 ethanol:diethyl phthalate was applied topically to the ear lobes of mice. Three days after the third topical application the mice were injected a buffered solution of 20µCi [3]H-methyl thymidine into the tail veins. Five hours later, the animals were killed, the lymph nodes excised and the desintegrations per minute (dpm) and the stimulation index (SI) determined.
Under the conditions of the study, dibenzyl ether is a weak sensitiser. The EC3 value was calculated to be 6.3% (1575 µg/cm²).
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