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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018.01.17 - 2018.02.14
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Tyrosine
- EC Number:
- 200-460-4
- EC Name:
- Tyrosine
- Cas Number:
- 60-18-4
- Molecular formula:
- C9H11NO3
- IUPAC Name:
- tyrosine
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L, and control.
- Sampling method: taken from the limit test concentration and control at test start and after 24 hours from fresh and aged solutions and after 48 hours from aged solutions. For each sampling also a retain sample was taken.
- Sample storage conditions before analysis: stored deep frozen
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution S1: weighed necessary amount of test item to a volumetric flask, added test medium up to the bench mark and then homogenised by shaking and 45 min of ultrasonication.
- Evidence of undissolved material: the solution was clear and transparent
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain/clone: Daphnia magna (Straus), clone V
- Source: originally purchased from the Federal Environment Agency in Berlin/Germany
- Age at Test Start: Less than 24 hours
- Breeding Conditions: feed with single cell green algae (Desmodesmus subspicatus) at least three times a week.
- Feeding during test: no
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 232 mg CaCO3/L
- Test temperature:
- 18.9 to 21.9 °C
- pH:
- 7.58 – 7.79
- Dissolved oxygen:
- 7.8 - 9.2 mg/L
- Nominal and measured concentrations:
- Nominal: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass vessel (100 mL)
- Type: closed
- Fill volume: filled up with ~ 50 mL test solution
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: ~ 10 mL of test solution for each animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- pH: 7.68
- Dissolved oxygen concentration: 9.2 mg/L
- Total hardness:13°dH (232 mg/L as CaCO3)
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod /8 hours darkness daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Biological Assessment: after 24 h and 48 h: count the immobilised daphnids and record behavioural changes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Biological Assessment: After 24 and 48 hours of exposure no immobilisation was observed in the control and at 100 mg/L test item. No behavioural changes could be observed.
- Analytical Results: The initial measured content of test item was between 94 and 95 % of nominal and the aged measured content was 91 % of nominal. - Results with reference substance (positive control):
- EC50 (24 h) < 1.00 mg/L, in accordance with the requirements of the OECD guideline 202 and fall within the historical data, the daphnids were suitable for the determination of the toxicological effects of the test item.
- Reported statistics and error estimates:
- Due to an inhibition below 50% the database was weak for probit analysis which hence was not performed
Any other information on results incl. tables
Results of the test, 48 h values:
| Nominal test item concentration [mg/L] | |
Control | 100 | |
Immobilised daphnids after 48 h | ||
Group 1 | 0 | 0 |
Group 2 | 0 | 0 |
Group 3 | 0 | 0 |
Group 4 | 0 | 0 |
Total | 0 | 0 |
% | 0 | 0 |
EC50and NOEC-values of daphnids exposed to the test item evaluated using nominal concentrations:
Endpoint | Test item [mg/L] | |
24 h | 48 h | |
NOEC | 100 | 100 |
EC50 | >100 | >100 |
95% confidence limit of EC50 | - | - |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the results of the test, the EC50 (48 h) for immobilisation was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
- Executive summary:
O-phospho-L-tyrosine dissociates in aqueous solutions. As according to REACH regulation only publicly available data has to be provided with the Registration dossier for on-site isolated intermediates in a tonnage band of < 1,000 t/a. Thus, information on the paren compound L-tyrosin is considered relevant and will be used for the registration dossier of O-phospho-L-tyrosine.
The immobilisation effect of the test item on the water flea Daphnia magna was determined under worst-case exposure conditions according to OECD 202 (2004).
Semi - static limit test (100 mg/L) with twenty organisms per test concentration (4 replicates of 5 animals each) were used. The duration of the test was 48 hours.
Analytical samples taken at 0 hours (initial value) and 24 hours from fresh and at 48 hours from aged test solutions were analysed from control and the limit test concentration.
The initial measured content of test item was between 94 and 95 % of nominal and the aged measured content was 91 % of nominal. The toxicological endpoints were evaluated using nominal concentrations.
According to the results of the test, the EC50 (48 h) was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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