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EC number: 419-050-3 | CAS number: 79944-37-9 AMINODIOXEPAN
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3 Apr - 7 Apr 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No analytical measurements were performed during the study to prove test concentrations. However, the study on water solubility (see 4.8) showed that a sufficient water solubility of 340 g/L is given for the test substance to reach the maximum concentration of 600 ppm that were used within this study. The study on acute toxicity to daphnia magna (see 6.1.3) also indicates that the substance concentration remains constant over 48 h (measured concentration 93-94% of nominal concentration). Furthermore, the study on inherent biodegradability (see 5.2.1 OECD 302 B) showed that only 20% of the test substance were subject to abiotic degradation within 20 days. Therefore, the concentration of the test substance within this study is considered to be sufficient stable and results remain valid.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Please see 'Rationale for reliability incl. deficiencies'
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the test concentrations a stock solution was prepared by adding 15.0 g of the test substance to 2 L Millipore water which was then diluted in a series to prepare the test solutions.
- Controls: 1 control
- Test concentration separation factor: 1.5 to 2.0 - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: reputable supplier
- Weight at study initiation: 0.22 +/- 0.05 g
ACCLIMATION
- Acclimation period: 1 month
- Type and amount of food during acclimation: commercial fish food 0.4 g/ 100 fish
- Feeding frequency during acclimation: daily
- The water was changed with newly performed dilution water (SF No. 30/510) every 6th day.
FEEDING DURING TEST (as applicable)
- Food type: Food is withheld throughout the test period. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21.4 °C to 22.0 °C
- pH:
- 7.7 to 8.0
- Dissolved oxygen:
- 95 % to 100 %
- Nominal and measured concentrations:
- nominal concentrations: 0, 50, 100, 200, 400, 600 ppm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Each test vessel was filled with 10 L
- Material, size, headspace, fill volume: polyethylene aquaria with a total capacity of 20 L and a surface area of 800 cm2
- Aeration: test vessels were aerated gently
- No. of organisms per vessel: 10 fish
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: newly produced dilution water (SF 30/510)
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Photoperiod: daily light/dark period 14:10 h
EFFECT PARAMETERS MEASURED
- Mortalities are recorded after 2, 24, 48, 72 and 96 hours.
- Additional observation of symptoms: swimming behavior, loss of equilibrium, seek to the bottom, respiration, pigmentation
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5 to 2.0
- Range finding study: test concentrations were selected on the basis of a preliminary rankfinding test (results not reported)
- Test concentrations: 0, 50, 100, 200, 400, 600 ppm - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 600 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC10
- Effect conc.:
- > 600 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other adverse effects control: 0%
- Mortality of control: 0%
- Abnormal responses: after 24 h one fish seek to bottom in the 100, 200, 400 and 600 ppm concentration replicate and after 72 h and 96 h one fish seek to bottom in the 600 ppm concentration replicate. - Results with reference substance (positive control):
- LC50 was found to be 330 mg/L for the reference substance Potassium dichromate (K2Cr2O7)
- Sublethal observations / clinical signs:
Validity criteria for the measurement of the fish toxicity
Target condition according to guideline: Actual condition according to the study: Validity criteria met: In the control(s) (dilution water control, solvent control), the mortality should not exceed 10% (or one fish, if fewer than 10 control fish are tested) at the end of the exposure. Mortality of control: 0 fish died Yes The dissolved oxygen concentration should be >/= 60% of the air saturation value in all test vessels throughout the exposure period. The oxygen saturation was measured in a 24 hour interval and ranged between 95% to 100%. Yes The concentration of the test item shall be maintained to within 80% of the initial concentration throughout the study. The maintenance of concentration is proved by analytical measurements No analytical measurements were performed during the study to prove test concentrations. No Temperature: the water temperature should not differ by more than 2°C During the test a temperature range of 21.4 to 22.0°C was maintained in the test vessels.
Yes The pH should not vary by more than one unit During the test, the measured pH values in the test vessels were in the range of 7.7 to 8.0.
Yes - Validity criteria fulfilled:
- no
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- No toxic effects of the test substance against fish up to 600 ppm (nominal concentration) were observed; LC50(96h)>600 ppm (nominal concentration).
- Executive summary:
The study was performed to assess the acute toxicity of the test substance to zebra fish (Danio rerio former Brachydanio rerio) under static conditions. The study was conducted according to OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish were exposed to five test concentrations (50, 100, 200, 400, and 600 ppm), a control treatment and treatment with the reference substance Potassium dichromate. Observations were made on the number of dead fish and additional adverse symptoms after 24, 48, 72, and 96 hours of exposure. The following values were determined: LC50(96h)>600 ppm (nominal concentration). No analytical measurements were performed during the study to prove test concentrations. However, the study on water solubility (see 4.8) showed that a sufficient water solubility of 340 g/L is given for the test substance to reach the maximum concentration of 600 ppm that were used within this study. The study on acute toxicity to daphnia magna (see 6.1.3) also indicates that the substance concentration remains constant over 48 h (measured concentration 93-94% of nominal concentration). Furthermore, the study on inherent biodegradability (see 5.2.1 OECD 302 B) showed that only 20% of the test substance were subject to abiotic degradation within 20 days. Therefore, the concentration of the test substance within this study is considered to be sufficient stable and results remain valid.
Reference
Description of key information
The study was performed to assess the acute toxicity of the test substance to zebra fish (Danio rerio former Brachydanio rerio) under static conditions. The study was conducted according to OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish were exposed to five test concentrations (50, 100, 200, 400, and 600 ppm), a control treatment and treatment with the reference substance Potassium dichromate. Observations were made on the number of dead fish and additional adverse symptoms after 24, 48, 72, and 96 hours of exposure. The following values were determined: LC50(96h)>600 ppm (nominal concentration). No analytical measurements were performed during the study to prove test concentrations. However, the study on water solubility (see 4.8) showed that a sufficient water solubility of 340 g/L is given for the test substance to reach the maximum concentration of 600 ppm that were used within this study. The study on acute toxicity to daphnia magna (see 6.1.3) also indicates that the substance concentration remains constant over 48 h (measured concentration 93-94% of nominal concentration). Furthermore, the study on inherent biodegradability (see 5.2.1 OECD 302 B) showed that only 20% of the test substance were subject to abiotic degradation within 20 days. Therefore, the concentration of the test substance within this study is considered to be sufficient stable and results remain valid.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 600 mg/L
Additional information
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