Reaction products of N-Phenyl-1-Naphthylamine with Nonene, branched

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2021-06-23 - 2021-06-30

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

CAS No.: 63451-49-0
Purity: UVCB, sum of all peaks: 100.00 area-%
Identity: confirmed
Storage stability: Expiry date: 18 Nov 2022
The stability of the test substance under storage conditions over
the test period was guaranteed by the sponsor, and the sponsor
holds this responsibility.
The test facility is organizationally independent from the BASF SE sponsor division.

Chemical name: 1-Naphthalenamine, N-(nonylphenyl)-
Date of production: 19 Nov 2019
Appearance
- physical state: liquid, viscous
- color:brown to red
Water solubility: At 20.0 °C ±0.5°C:
0.7 μ/L ±0.5 μg/L for tests with 0.1 g/L and 0.7 μg/L ±0.3 μg/L

Analytical monitoring:

yes

Details on test solutions:

The test substance is poorly water soluble, therefore a water accommodated fraction (WAF) was prepared using a liquid-liquid saturator technique . A glass aspirator bottle (2-L) with a bottom side-outlet attached to a stopcock was filled with 2-L test media. The test medium was stirred at approx. 100 rpm and
then 198-μL (considering density = 1.009 g/cm³) of the test substance was pipetted (Gilson Microman E M 1000E) carefully on the water surface. The bottle was closed, and the solutions were stirred on a magnetic plate for approximately 3 days. The stirring was slow (approx. 100 rpm) so that no vortex forms. The remaining undissolved test substance remained on the surface and the
required volume of the WAF (approx. 1-L per test solution) was removed from the bottom port. The solutions were visually inspected for the presence of any undissolved test substance.
Undissolved test substance was visible at the surface of the test aspirator bottle (brownish drops). Then the aqueous fraction was inspected visually for the presence of any undissolved test substance by observing the scattering of a laser light through the test solution (Tyndall effect). The Tyndall effect was negative.
The control was treated in the same way.
Fresh test solutions were prepared daily. The test vessels were saturated with the test solution for approx. 24-h prior to initiating exposure.
All test solutions were visibly clear over the exposure period. The given water solubility is 0.7 μ/L ±0.5 μg/L . In test media, the detectable concentrations were much less (approx. 0.02-0.66 μg/L. OECD guidance acknowledges that differences in solubility between pure water and aqueous test media (containing nutrient salts) are not unusual. All reasonable efforts were taken to produce a water accommodated fraction (water saturated fraction) of the test substance according to guidance in OECD 23. The aqueous fraction of the test solution, after separation of the undissolved material, is considered analogous to a saturated solution of the test substance. No higher concentration of the test substance in test medium could be achieved.
Since all samples demonstrated the presence of the test substance in solution, the overall mean measured concentrations calculated are considered to accurately represent exposure concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

Animal supplier:: The clone of Daphnia magna STRAUS 1820 used in this study was originally obtained from the Institut National de Recherche
Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of the BASF SE, Ludwigshafen, Germany.
Culture conditions: Daphnia brood stock are kept in mass cultures consisting of
approx. 20 – 30 parthenogenetically reproducing females for a maximum of 4 weeks and fed live unicellular algae (Desmodesmus subspicatus), cultured continuously at the test facility. All individuals in each mass culture originate from a single female and are thus genetic clones. After approximately 14 days the adults have produced at least 3 broods and the young can be used in tests. Offspring are removed from the mass cultures at least once daily during the normal work week to ensure that young daphnia are <24-h old (first instar) at start of the exposure. Detailed records are kept (in test facility archives) to monitor the health of Daphnia brood stock cultures including observations of young production, mortality, ephippia, and measurement of water chemistry parameters. Only young from healthy cultures without signs of stress are used for testing.
Acclimatization: The Daphnia are cultured under the identical conditions as the
test including test media (Elendt M4), water quality, and temperature (20 ±1°C).
Age at start of exposure: <24 hours (at least 3rd brood progeny)
Reference substance testing:
In order to verify that the Daphnia magna culture is responding normally to toxic stress, tests with a reference substance, sodium chloride, are conducted monthly. Reference substance tests are conducted according to OECD 202 guidelines and in accordance with GLP, but without a GLP status.
The EC50(48h) of the reference substance sodium chloride was 4.73 g/L (experiment date: 21Jul 2021, project number: 50E0789/12E067).
This result is within the range of 3.88 – 7.22 g/L (Ref. 12) and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress.
HOUSING AND DIET
Culture media: synthetic fresh water (Elendt M4) prepared according to OECD
202.
Housing:
Temperature: 20 ±1°C
Photoperiod: 16 hours light, 8 hours dark
Diet: Unicellular green algae, Desmodesmus subspicatus, (cultured continuously at the testing laboratory)
Feeding: Daily
Renewal of the culture water:Twice weekly

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Nominal and measured concentrations:

The test substance is poorly water soluble, therefore a water accommodated fraction (WAF) was prepared using a liquid-liquid saturator technique.

Details on test conditions:

A glass aspirator bottle (2-L) with a bottom side-outlet attached to a stopcock was filled with 2-L test media. The test medium was stirred at approx. 100 rpm and then 198-μL (considering density = 1.009 g/cm³) of the test substance was pipetted (Gilson Microman E M 1000E) carefully on the water surface. The bottle was closed, and the solutions were stirred on a magnetic plate for approximately 3 days. The stirring was slow (approx. 100 rpm) so that no vortex forms. The remaining undissolved test substance remained on the surface and the required volume of the WAF (approx. 1-L per test solution) was removed from the bottom port. The solutions were visually inspected for the presence of any undissolved test substance. Undissolved test substance was visible at the surface of the test aspirator bottle (brownish drops). Then the aqueous fraction was inspected visually for the presence of any undissolved test substance by observing the scattering of a laser light through the test solution (Tyndall effect). The Tyndall effect was negative.

EXPERIMENTAL PROCEDURE
Test medium: A synthetic fresh water (Elendt M4) is used as media for culture
and test purposes. For the composition of this M4 medium see OECD 202. The general properties of this medium are as follows.
Total hardness: 2.20 – 3.20 mmol/L
Acid capacity up to pH 4.3: 0.80 – 1.00 mmol/L
Molar ratio Ca:Mg: about 4 : 1
pH value: 7.5 – 8.5
Conductivity: 550 - 650 μS/cm
Total organic carbon: <2 mg/L
Dissolved oxygen: Must remain 3mg/L during the test. To assure optimal dissolved oxygen levels, the M4 medium is aerated for approximately 24 hours prior to use.
Test vessels: Numbered glass beakers (nominal volume 100 mL)
Test volume: 50 mL
Test solution renewal: Static-renewal exposure via test water.
Reason:
In order to insure, constant exposure conditions this study was conducted as a static-renewal exposure. The renewal period was 24 hours.
Test temperature: 19.5 – 20.1 °C (continuous monitoring)
Biological loading: 5 animals / test vessel (0.1 animals/mL)
Light intensity / Photo
period:
184-772 lux at a wavelength of 400-750 nm;
16 hours light : 8 hours darkness
Aeration: none
Diet: No feeding during the exposure period.
The exposure was started by impartially distributing 20 neonate (< 24 hours old) Daphnia magna evenly among the 4 test vessels per test group. The neonate daphnids all originated from the same mass culture and were thus genetic clones. The Daphnia were transferred into the test vessels with minimal culture water by capturing each daphnid in a pipette then gently expelling it onto a Teflon mesh (70 μm) held by forceps. Each daphnid was then immediately transferred from the mesh into the corresponding test vessel from lowest to highest concentration.
Each test vessel was visually checked for immobilized daphnids after 0, 24 and 48 hours. In addition, any abnormal behavior or appearance was documented.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.223 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

other:

Details on results:

The following effect concentrations (μg/L) were obtained after 48 hours based on overall mean measured concentrations:
EC50 > 0.223 μg/L
No toxic effects were observed in the saturated solutions with a loading rate of 100 mg/L up to the solubility limit of the test substance.
Since the measured concentrations deviated markedly from the nominal concentrations, the effect concentration, which is based on the overall mean measured concentrations should be preferably used for the evaluation of the test substance.

Validity criteria fulfilled:

yes

Conclusions:

In an OECD 202 test with Daphnia magna the test item displayed no toxicity within the range of the water solubility in test media. The test item is not acute toxic toward Daphnia magna.

Executive summary:

In an OECD 202 test with Daphnia magna the test item displayed no toxicity within the range of the water solubility in test media. The test item is not acute toxic toward Daphnia magna.

Description of key information

In an OECD 202 test with Daphnia magna the test item displayed no toxicity within the range of the water solubility in test media. The test item is not acute toxic toward Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Remarks:

loading rate

Effect concentration:

> 100 mg/L