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EC number: 204-634-0 | CAS number: 123-54-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980-04-23 to 1980-04-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study well documented meeting generally accepted principles
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- see Principles
- Principles of method if other than guideline:
- 25 fishes per group; Lake Superior water; 5 concentrations (28.5-295 mg/l) and control with duplication were tested, analytical control by GLC in 24 h intervals up to 96 h; the tank volume was 6,3 l.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Analytical monitoring:
- yes
- Details on sampling:
- chemical analysis by Gas-Liquid chromatography (GLC)
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: undiluted injection
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Fathead minnows used in the tests were cultured from brood stock provided by the U.S. EPA Environmental Research Laboratory-Duluth. Adults were held at 25 °C in flowing water at 16 hr light controlled-photoperiod and fed frozen adult brine shrimp (Artemia sp.). They were provided with asbestos pipe (cut in half longitudinally) as spawning substrate. Eggs were spawned and fertilized on the undersides of these substrates. The embryos were tended by the male parent until eye-up at which time the substrates with embryos attached were removed to another 25 °C bath where hatching occurred.
Fry of similar age were transferred to flow-through rearing tanks at 25 °C with a 16 hr light pho-toperiod. Fry were fed freshly hatched brine shrimp nauplii to excess three times daily until 24 hr before a test (twice on most week ends) and a dry flake ration during their first week. Fish that were approximately 30 days old were used in the toxicity tests.
Only groups of fish having healthy appearance and no history of unusual thermal exposure or abnormally high mortality rate were used for toxicant exposures. Fish were not fed during chemical exposures.
Mean length: 21.8 mm (+/- 2.386 mm)
Mean weight: 0.145 g (+/- 0.0486 g)
Loading rate: 0.575 g/l - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- not reported
- Hardness:
- hardness 46.2 mg/l CaCO3
- Test temperature:
- 25.2 °C
- pH:
- pH 7.37
- Dissolved oxygen:
- 6.3 mg/l dissolved oxygen
- Nominal and measured concentrations:
- 0, 60.5, 101, 168, 280 and 467 mg/l (nominal);
0; 26.2 and 35.0; 56.0 and 57.2; 87.9 and 96; 145 and 152, 269 and 271 mg/l ( mean measured in duplicate replicates) - Details on test conditions:
- Prior to initiating a test, prospective test fish were pooled if reared in more than one tank, and by inspection unusually large or small individuals were netted out with minimal disturbance to the remainder of the fish. At the start of a test, fish were removed from the “common pool” of fish with a beaker or net and distributed at random, among the exposure chambers.
During exposure to toxicants the fish were routinely observed for behavioral responses (effects) and deaths. Early tests were sometimes without effect observations. The concentration of the highest tested dose were at the limit of water solubility. Death was defined as the cessation of opercular movements and the inability to respond when prodded. Dead fish were removed and recorded at 3, 6, 12, 24, 48 72 and 96 hr from initial exposure.
At the termination of tests, control fish were weighted (wet) to the nearest mg after blotting ex-cess water from them with a paper towel and measured (standard length) to the nearest mm.
Flow-through exposures were conducted with cycling proportional diluters with duplicate exposures for every tested concentration. Toxicants were proportionally diluted with Lake Superior water from stock solutions before delivery to fish exposure chambers. Duplicated exposures were performed. Test chemicals were prepared by undiluted injection of the test substance. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 104 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 98.3 - 110 mg/l (95% CL)
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 68.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: 64.3 - 71.9 mg/l (95% CL)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 57.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 145 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The number of mortalities was noted every 24 h after beginning of the test, at which time they were also removed.
The estimated LC 50 with corresponding 95 % confidence interval (98,3 - 110 mg/l) was calculated using the corrected average of the analyzed tank concentrations.
Affected fish became hyperactive and then lost equilibrium prior to death. - Reported statistics and error estimates:
- Estimation of the LC50 and EC50 was made using the trimmed Spearman-Karber” method. Calculations were made for 96 hr of exposure and also for intermediate exposure times using mean exposure concentrations of toxicants for the duration of the test. Calculations of means ands standard deviations were rounded to the same precision as the original determinations.
- Conclusions:
- Substance is not harmful to fish.
- Executive summary:
2,4-Pentanedione (> 99% pure) was assessed in an 96 h flow through acute toxicity test on Pimephales promelas according to a guideline similar to OECD 203. Five concentrations (28.5-295 mg/l) and control with duplication were tested on groups of 25 fish which were 30 days old at the beginning of the test. The concentration of the test item was monitored daily by analytical gas liquid chromatography showing a recovery rate of 108% (mean). The test showed mortality in groups at or exceeding 87.9 mg/l and all fish were found dead at 96 h at concentrations of the test item of or exceeding 145 mg/l. The EC50 based on behavioral effects was 68.5 mg/l with a confidence interval of 64.3 to 71.9 mg/l whereas the LC50 was 104 mg/l (98.3 to 110 mg/l 95% confidence interval).
Reference
Description of key information
In the study of Brooke et al. (1984) 2,4-Pentanedione (> 99% pure) was assessed in an 96 h flow through acute toxicity test on Pimephales promelas according to a guideline similar to OECD 203. The test showed mortality in groups at or exceeding 87.9 mg/l and all fish were found dead at 96 h at concentrations of the test item of or exceeding 145 mg/l. The EC50 based on behavioral effects was 68.5 mg/l with a confidence interval of 64.3 to 71.9 mg/l whereas the LC50 was 104 mg/l (98.3 to 110 mg/l 95% confidence interval).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- EC50
- Effect concentration:
- 68 mg/L
Additional information
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