mixture of two components: 1. N-(1,3- dimethylbutyl)-N´- phenyl-p-phenylenediamine 2. N1-(1,3-dimethylbutyl)- N4-(4-(1-methyl-1- phenylethyl)phenyl)ben zene-1,4-diamine

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-3-11 - 2005-12-21

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

other: Body responsible for the test

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: C.8 Toxicity for earthworms : artificial soil test OECD 207: OECD Guideline for testing of Chemicals "Earthwor, Acute Toxicity Test"

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Batch No: 26/2004

Test substrate

Vehicle:

yes

Details on preparation and application of test substrate:

Acetone

Test organisms

Test organisms (species):

other: Eisenia foetida

Details on test organisms:

TEST ORGANISM
- Common name:
- Source: Aqua-tera Svatopluk Ondrej Pozděchov, Czech Republik
- Age at test initiation (mean and range, SD): 2-3 months
- Weight at test initiation (mean and range, SD): 308 573 mg


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): no mortality

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20+/-2°C

Details on test conditions:

Soil characteristics (e.g. pH, % organic matter):
Basic substrate compound:

- 10% peat [ pH 5.5-6.0, moisture content 45-63% ]

- 20% china clay which consist from more then 50% kaolin

- 69-70% industrial silicic sand

- 0-1% calcium carbonate [powdery, chemically pure for pH
adjustment to 6.0+/-0.5]

All basic substrate compounds are mixed thoroughly and in
this homogenized mixture dried matter was analysed at
temperature 105deg. C = 87.1 % w/w

[ analysed by Sartorius MA 30 moisture analyzer ]


Artificial soil substrate compound:

For toxicity test on earthworm the defined artificial soil
was used as a supporting medium with demanded moisture

25-42%, consist of basic substrate and deionized water to
obtain adequately moist soil

-reference matter : chloroacetamide [ NH2-CO-CH2-Cl ]p.a.

-auxiliary matter: acetone p.a.

Nominal and measured concentrations:

preliminary test 0.1 - 1 - 10 - 100 - 1000 mg/kg dried matter
basic test: 640 - 800 - 1000 - 1280 - 1600 - 2000 mg/kg dried matter

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

1 463 other: mg/kg

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

800 other: mg/kg

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

LC100

Effect conc.:

> 2 000 other: mg/kg

Basis for effect:

mortality

Results with reference substance (positive control):

LC50= 18.4 mg/kg dried matter