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EC number: 603-520-1 | CAS number: 131807-57-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: terrestrial
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation: terrestrial
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 317 (Bioaccumulation in Terrestrial Oligochaetes)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Substance ID: DPX-JE874-496
Lot #: MAR15MB104
Purity: 98.2% - Vehicle:
- yes
- Remarks:
- Acetone, fine quartz sand
- Test organisms (species):
- Eisenia andrei
- Total exposure / uptake duration:
- 21 d
- Total depuration duration:
- 21 d
- Test temperature:
- 20 ± 2 °C
- pH:
- At start of the uptake phase: 5.6 to 6.0
at end of the uptake phase: 5.8 to 6.0
at start of the elimination phase: 5.7 to 5.8
at end of the elimination phase: 5.9 to 6.1 - Moisture:
- Water content: At start of the uptake phase: 32.6% to 34.4%
(equivalent to 47.2% to 49.9% WHC)
at the end of the uptake phase: 28.6% to 30.6%
(equivalent to 41.4% to 44.3% WHC)
at start of the elimination phase: 36.3% to 36.4%
(equivalent to 52.6% to 52.8% WHC)
at the end of the elimination phase: 31.8% to 33.1%
(equivalent to 46.1% to 48.0% WHC) - Nominal and measured concentrations:
- Nominal: 2 mg/kg soil (dry weight)
Measured: 1.69 mg/kg - Type:
- BCF
- Value:
- 0.369 dimensionless
- Basis:
- other: wet weight
- Calculation basis:
- steady state
- Type:
- BCF
- Value:
- 1.813 dimensionless
- Basis:
- other: dry weight
- Calculation basis:
- steady state
- Type:
- BCF
- Value:
- 0.756 dimensionless
- Basis:
- other: Wet weight
- Calculation basis:
- kinetic
- Type:
- BCF
- Value:
- 3.364 dimensionless
- Basis:
- other: dry weight
- Calculation basis:
- kinetic
- Kinetic parameters:
- The uptake rate constant (ks) (wet weight) was 0.251 d-1 and the elimination rate constant in worm tissues (ke) was 0.332 d-1. The corresponding kinetic bioaccumulation factor BAFK (wet weight) was calculated to be 0.756. The uptake rate constant (ks) (dry weight) was 1.960 d-1 and the elimination rate constant in worm tissues (ke) was 0.583 d-1. The corresponding kinetic bioaccumulation factor BAFK (dry weight) was calculated to be 3.364.
- Conclusions:
- During the uptake phase, the concentration of the test substance in worm tissue peaked at Day 2, and then decreased and was 0.394 mg/kg (wet weight) at the end of the uptake phase. Steady state was reached by Day 10. Based on the analysed concentrations, the Steady state BAF was 0.369 (wet weight) and 1.813 (dry weight), and the Kinetic BAF was 0.756 (wet weight) and 3.364 (dry weight).
- Executive summary:
The residues of the test substance in earthworms, Eisenia andrei Savigny, were determined at intervals up to 21 days uptake phase in artificial soil in a GLP-compliant laboratory study based on OECD 317 (2010). The elimination of residues was investigated over a further 21-day period (elimination phase). 22 replicates for the control and 54 replicates for the test item groups of five clitellated adult earthworms each were exposed to artificial soil (prepared according to OECD 222 with 10% organic matter) treated with the test item to obtain the nominal concentrations of 2 mg/kg dry artificial soil (1.69 mg/kg analytically) and to an untreated control moistened with deionized water (deionized water only). No reference item is tested.
Adult earthworms were sorted from soil and assessed for mortality, weight loss, and concentration of the test substance (as total radioactivity) after different exposure time. After 21 days exposure, the worms were transferred to untreated soil. For the 21 days elimination phase worms were removed after 4 hours (h), and after 1, 2, 4, 7, 10, 14, 17 and 21 days (d) uptake.
All study validity criteria were met. Mortality was 0% at the end of the uptake and elimination phase and the mean loss of biomass did not exceed 20%. Cumulative mortality and weight loss of adults at sampling time, and food consumption are reported below for each treatment group. No mortality was observed in any treatment group. During the uptake phase, the measured concentration of the test substance in soil was 1.490 mg/kg soil (dry weight) at start of the uptake phase and 1.386 mg/kg soil (dry weight) after 21 days uptake phase.
The concentration of the test substance in worm tissues was in the range of 0.296 to 1.006 mg/kg wet weight during the uptake phase. In the test, the test substance was absorbed by the earthworms. 24 hours after start of exposure, 0.581 mg/kg worm tissue (wet weight, mean value) was found in earthworm tissue. Up to Day 7 of the uptake phase, the test item concentration in worm tissue ranged from 0.926 to 1.006 mg/kg (wet weight). At the following sampling occasions, the test item concentration in worm tissue was almost constant ranging from 0.296 to 0.495 mg/kg (wet weight).
After transferring the worm to untreated soil, the test item concentration in worm tissue decreased rapidly. After Day 1 of the elimination phase, the concentration was 0.189 mg/kg (wet weight) and was below limit of quantification (0.05 mg/kg wet weight) at Day 7 to the end of the elimination phase.
The steady state was reached after 10 days of exposure in the uptake phase. Successive analysis of BAF made on samples taken at following sampling occasions did not vary by more than 24%. The BAFss was calculated to 0.369 (wet weight) and 1.813 (dry weight). The uptake rate constant (ks) (wet weight) was 0.251 d-1 and the elimination rate constant in worm tissues (ke) was 0.332 d-1. The corresponding kinetic bioaccumulation factor BAFK (wet weight) was calculated to be 0.756. The uptake rate constant (ks) (dry weight) was 1.960 d-1 and the elimination rate constant in worm tissues (ke) was 0.583 d-1. The corresponding kinetic bioaccumulation factor BAFK (dry weight) was calculated to be 3.364.
During the uptake phase, the concentration of the test substance in worm tissue peaked at Day 2, and then decreased and was 0.394 mg/kg (wet weight) at the end of the uptake phase. Steady state was reached by Day 10. Based on the analysed concentrations, the BAFSS was 0.369 (wet weight) and 1.813 (dry weight), and the BAFK was 0.756 (wet weight) and 3.364 (dry weight).
Reference
Description of key information
Study Type | Study Details | Value | Guideline | Reliability |
Bioaccumulation: terrestrial | Accumulation and elimination in earthworms in artificial soil | BCF = 0.369 for wet weight; BCF = 1.813 for dry weight | OECD 317 | 1 |
Key value for chemical safety assessment
Additional information
The BCF study state was calculated to 0.369 (wet weight) and
1.813 (dry weight)
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