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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 8.4 - 13 mg/L WAF
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: NDA
- Observations on body length and weight: NDA
- Other biological observations: NDA
- Mortality of control: 0
- Other adverse effects control: None noted
- Abnormal responses: NDA
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None noted
- Effect concentrations exceeding solubility of substance in test medium: N/A - Results with reference substance (positive control):
- - Results with reference substance valid? Yes.
- Mortality: mortality was observed at concentration ≥ 0.56 mg/L
- EC50/LC50:
Time (h) 3
EC50 (mg/l) > 3.2
95% Confidence limits (mg/l) - no data
Time (h) 24
EC50 (mg/l) = 1.0
95% Confidence limits (mg/l) = 0.90 – 1.2
Time (h) 48
EC50 (mg/l) = 0.78
95% Confidence limits (mg/l) = 0.68 - 0.88 - Reported statistics and error estimates:
- No data available
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EL*50 value of 11 mg/l loading rate WAF with 95% confidence limits of 8.4 - 13 mg/l loading rate WAF. The No Observed Effect Loading rate at 48 hours was 1.8 mg/l loading rate WAF.
- Executive summary:
Introduction.A study was performed to assess the acute toxicity of the test material to daphnia magna followed that described in the OECD 202.
Methods.Following a preliminary range-finding test, twenty daphnids (2 replicates of
10 animals) were exposed to Water Accommodated Fractions (WAFs) of the test material over a range of nominal loading rates of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/l for 48 hours at a temperature of 21ºC to 22ºC under semi-static test conditions. The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
A positive control conducted approximately every six months used potassium dichromate as the reference material. Daphnia magna was exposed to an aqueous solution of the reference material at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/l for 48 hours at a temperature of 21°C to 22°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 3, 24 and 48 hours.
Results.The 48-Hour EL*50 for the test material to Daphnia magna based on nominal loading rates was 11 mg/l loading rate WAF with 95% confidence limits of 8.4 - 13 mg/l loading rate WAF. The No Observed Effect Loading rate was 1.8 mg/l loading rate WAF.
Chemical analysis of the test preparations at 0 (fresh media), 24 (old and fresh media) and 48 (old media) hours showed measured test concentrations to range from 0.015 mg/l to 0.265 mg/l. A concentration dependant general trend was consistently observed (with increasing loading rate) in both the fresh and old media at both media renewal periods, and a slight decline in measured concentration was consistently observed over each media renewal period.
The control test sample at 0 hours showed a measured concentration of 0.0030 mg/l.
Analysis of a frozen duplicate sample showed a measured concentration of less than the limit of quantification (LOQ) which confirmed the original analysis to be erroneous.
The dissolved test material may have been one or several components of the test material. Given that toxicity cannot be attributed to a single component or a mixture of components but to the test material as a whole, the results were based on nominal loading rates only.
The 48-Hour EC50 for the reference material to Daphnia magna based on nominal concentrations was 0.78 mg/l with 95% confidence limits of 0.68 – 0.88 mg/l. The No Observed Effect Concentration was 0.32 mg/l.
Reference
Cumulative Immobilisation data in the Range Finding Test
Nominal Loading Rate (mg/l) |
Cumulative ImmobilisedDaphnia(Initial Population: 10 Per Replicate) |
|
24 Hours |
48 Hours |
|
Control |
0 |
0 |
1.0 |
0 |
0 |
10 |
0 |
2 |
100 |
0 |
4 |
Cumulative Immobilisation Data in the Definitive Test
Nominal Loading Rate (mg/l) |
Cumulative Immobilised Daphnia (Initial Population: 10 Per Replicate) |
|||||||
24 Hours |
48 Hours |
|||||||
R1 |
R2 |
Total |
% |
R1 |
R2 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.8 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3.2 |
0 |
0 |
0 |
0 |
0 |
2 |
2 |
10 |
5.6 |
0 |
0 |
0 |
0 |
3 |
3 |
6 |
30 |
10 |
0 |
0 |
0 |
0 |
5 |
4 |
9 |
45 |
18 |
0 |
0 |
0 |
0 |
6 |
7 |
13 |
65 |
32 |
0 |
0 |
0 |
0 |
9 |
9 |
18 |
90 |
56 |
0 |
0 |
0 |
0 |
10 |
10 |
20 |
100 |
100 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
Chemical analysis of test loading rates
Chemical analysis of the test preparations (see Appendix 3) at 0 (fresh media), 24 (old and fresh media) and 48 (old media) hours showed measured test concentrations to range from 0.015 mg/l to 0.265 mg/l. A concentration dependant general trend was consistently observed (with increasing loading rate) in both the fresh and old media at both media renewal periods, and a slight decline in measured concentration was consistently observed over each media renewal period.
The control test sample at 0 hours showed a measured concentration of 0.0030 mg/l. Analysis of a frozen duplicate sample showed a measured concentration of less than the limit of quantification (LOQ) which confirmed the original analysis to be erroneous.
The results of the chemical analysis showed measured concentrations in the definitive test that were lower than the measured Total Organic Carbon (TOC) levels observed in the pre-study validation of mixing period work. This was considered to be due to the analytical method employed only detecting the 399 ion whereas TOC analysis detected the total dissolved test material present in the WAF.
Description of key information
The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EL*50 value of 11 mg/l loading rate WAF with 95% confidence limits of 8.4 - 13 mg/l loading rate WAF. The No Observed Effect Loading rate at 48 hours was 1.8 mg/l loading rate WAF.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 11 mg/L
Additional information
A study was performed to assess the acute toxicity of the test material to daphnia magna followed that described in the OECD 202.
Following a preliminary range-finding test, twenty daphnids (2 replicates of
10 animals) were exposed to Water Accommodated Fractions (WAFs) of the test material over a range of nominal loading rates of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/l for 48 hours at a temperature of 21ºC to 22ºC under semi-static test conditions. The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
A positive control conducted approximately every six months used potassium dichromate as the reference material. Daphnia magna was exposed to an aqueous solution of the reference material at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/l for 48 hours at a temperature of 21°C to 22°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 3, 24 and 48 hours.
The 48-Hour EL*50 for the test material to Daphnia magna based on nominal loading rates was 11 mg/l loading rate WAF with 95% confidence limits of 8.4 - 13 mg/l loading rate WAF. The No Observed Effect Loading rate was 1.8 mg/l loading rate WAF.
Chemical analysis of the test preparations at 0 (fresh media), 24 (old and fresh media) and 48 (old media) hours showed measured test concentrations to range from 0.015 mg/l to 0.265 mg/l. A concentration dependant general trend was consistently observed (with increasing loading rate) in both the fresh and old media at both media renewal periods, and a slight decline in measured concentration was consistently observed over each media renewal period.
The control test sample at 0 hours showed a measured concentration of 0.0030 mg/l.
Analysis of a frozen duplicate sample showed a measured concentration of less than the limit of quantification (LOQ) which confirmed the original analysis to be erroneous.
The dissolved test material may have been one or several components of the test material. Given that toxicity cannot be attributed to a single component or a mixture of components but to the test material as a whole, the results were based on nominal loading rates only.
The 48-Hour EC50 for the reference material to Daphnia magna based on nominal concentrations was 0.78 mg/l with 95% confidence limits of 0.68 – 0.88 mg/l. The No Observed Effect Concentration was 0.32 mg/l.
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