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EC number: 404-170-0 | CAS number: 70750-63-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study; well-documented study report
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- Analytical purity: 81%
- Lot/batch No.: F70305T
- Expiration date of the lot/batch: 2006
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Method: The test material was weighed into cups constructed of aluminum foil and the cups an the test substance were added to the respective test vessels which contained reverse osmosis water and synthetic sewage. The vessels were placed in an ultrasonic bath (Decon Fs400b) and their contents subjected to ultrasonification for approximately ten minutes to disperse the test substance in the test medium before adding the inoculum
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Method of cultivation: Collected from one of the return lines of Burley Menston sewage treatment works. The sample was aerated by means of a compressed air supply delivered through a diverse block
- Preparation of inoculum for exposure: The inoculum was used in the range-finder two days after the sludge sample was collected. The inoculum was maintained by aeration at all times and was fed overnight with 400 mL synthetic sewage on two occasions during the interval between collection and use.
- Initial biomass concentration: 4 g/L (initials suspended solids concentration was 4.56g/L therefore it was diluted with de-chlorinated tap water to provide 8L inoculum with a nominal solids content of 4 g/L)
Study design
- Test type:
- other: Direct addition
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 19.4 to 21.6 C
- pH:
- Bulk inoculum pH was 6.09
- Nominal and measured concentrations:
- 1.0, 10, 100, and 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 500mL total (300 mL of synthetic sewage sludge (16mL) diluted with reverse-osmosis water and 200 mL of activated sludge)
- Aeration: yes
- No. of vessels per concentration (replicates): duplicate
- No. of vessels per control (replicates): unreplicated
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reverse-osmosis water
OTHER TEST CONDITIONS
- Adjustment of pH: used 1M NaOH
TEST CONCENTRATIONS
- Test concentrations: 1.0, 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study: Absence of significant toxic effect up to 1000 mg/L in the range finding test, therefore further testing was considered unnecessary.
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: The test substance did not inhibit the respiration of activated sludge at concentrations up to and including 1000 mg/L. The EC50 of the test substance could not be determined, but it exceeded 1000 mg/L.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 3,5 dichlorophenol EC50 was 9 mg/L
Any other information on results incl. tables
Treatment |
Log10conc |
Respiration rate mg O2/Lh |
Mean |
Standard Deviation σn-1 |
Percentage Inhibition |
Control |
na |
77.251 |
78.83 |
2.0 |
Na |
|
na |
78.251 |
|
|
|
|
na |
81.042 |
|
|
|
|
na |
90.112,3 |
|
|
|
1.0 mg/L Test material |
0.0000 |
84.38 |
83.90 |
0.7 |
0 |
|
|
83.41 |
|
|
|
10 mg/L Test material |
1.0000 |
85.27 |
84.94 |
0.5 |
0 |
|
|
84.61 |
|
|
|
100 mg/L Test material |
2.0000 |
88.47 |
85.06 |
4.8 |
0 |
|
|
81.64 |
|
|
|
1000 mg/L Test Material |
3.0000 |
89.27 |
84.54 |
6.7 |
0 |
|
|
79.80 |
|
|
|
5mg/L 3,5-DCP |
0.6990 |
53.09 |
na |
na |
33 |
15mg/L 3,5-DCP |
1.1761 |
28.12 |
na |
na |
64 |
45mg/L 3,5-DCP |
1.6532 |
6.28 |
na |
na |
92 |
|
|
|
|
|
|
1start-of-series control
2end of series control
3outside 15% acceptance limit. Omitted from mean
na: not applicableApplicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance did not inhibit the respiration of activated sludge at concentrations up to and including 1000 mg/L. The EC50 of the test substance could not be determined, but exceeded 1000 mg/L.
- Executive summary:
The toxicity to microorganisms of the test material was tested using activated sludge of a predominately domestic sewage according to OECD guideline 209. In the range-finder phase study, doses of 1.0, 10, 100, and 1000 mg/L (nominal) test material was added to the inoculum. The test substance did not inhibit the respiration of activated sludge at any concentrations tested, and further testing was considered unnecessary. Both the OECD guideline validity criteria that related to the reference inhibitory response and conformity of the control respiration rates were met. The results of this study are considered valid. The EC50 of the test substance could not be determined from the results of this study, but it exceeded 1000 mg/L. Based on these results the test article is considered to be non-toxic to microorganisms at the concentrations tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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