2-propen-1-amine, N-2-propen-1-yl-, hydrochloride

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

3-6 April 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

yes

Remarks:

The vessel temperature in the range-finding test was outwith the 21 – 24 °C (±2 °C) range.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

66% purity
Aqueous solution

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: 278/4
- Source: stock laboratory cultures
- Method of cultivation: autoclaved freshwater at 120 °C for 15 minutes with nutrient medium (3N-BBM+V) 7 days before test commencement. They were then incubated at approximately 21 2 °C under constant illumination to be used as the inoculum source for subsequent toxicity tests. The initial inoculum was checked microscopically using a haemocytometer.

ACCLIMATION
- Acclimation period: 7 days
- Culturing media and conditions same as test
- Any deformed or abnormal cells observed: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

Not specified

Test temperature:

21 to 24 °C controlled at 2 °C

pH:

7.5 ±0.1. Not increased during the test by more than 1.5 relative to the pH of the growth medium

Dissolved oxygen:

Not specified

Salinity:

Not specified

Conductivity:

Not specified

Nominal and measured concentrations:

1, 3.2, 10, 32, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass conical flasks
- Material, size, headspace, fill volume: borosilicate, 100ml
- Aeration: flasks were loosely covered with aluminium foil caps and mounted on an orbital shaker
- Initial cells density: approximately 5 x 10^3 – 10^4 cells per millilitre
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes, US EPA culture medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The purified water used in this test was treated by reverse osmosis followed by electro-deionisation. This process produces water of an equivalent grade to deionised water. The purified water was held in two 75 litre header tanks until use.

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: pH of the culture medium was adjusted if required, by adding 1M HCl, or NaOH to give a pH of 7.5 ±0.1.
- Light intensity and quality: Between 4440 to 8880 lx. The illumination consisted of 40 W cool white LED light which was mounted at a distance of approximately 40 cm directly above the test area.

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study: The range-finding test employed four concentrations 1000, 100, 10 and 1 mg/l with one replicate per concentration; plus, four controls
- Test concentrations: The definitive test employed five concentrations (1, 3.2, 10, 32, 100 mg/l) with three replicates per concentration; plus, six controls.
- Results used to determine the conditions for the definitive study: Calculated 72h EC(r)50 in the range-finding test was 5.33 mg/l

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 5.11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

ca. 9.02 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 3.43 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 1.42 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50: 1.16 mg/L

Reported statistics and error estimates:

Results for both range-finding and definitive tests were calculated by linear regression within the CETIS suite of statistical analysis.

Validity criteria fulfilled:

yes

Conclusions:

The 72-hour EC50 was ca. 5.11 mg/L and the 72-hour NOEC was ca. 1 mg/L.

Executive summary:

The aquatic phase toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater unicellular algae Pseudokirchneriella subcapitata was determined during a GLPcompliant study performed according to the OECD Testing Guideline 201.

A range-finding test was conducted over 72h to determine the approximate concentrations at which growth rate effects were observed.

The range-finding test employed four concentrations 1000, 100, 10 and 1 mg/L with one replicate per concentration; plus, four controls.

As a result of this range-finding test, concentrations of 1, 3.2, 10, 32, 100 mg/L were used during the definitive test.

The 72-hour EC50 was ca. 5.11 mg/L and the 72-hour NOEC was ca. 1 mg/L. 

Description of key information

The aquatic phase toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater unicellular algae Pseudokirchneriella subcapitata was determined during a GLPcompliant study performed according to the OECD Testing Guideline 201.

A range-finding test was conducted over 72h to determine the approximate concentrations at which growth rate effects were observed.

The range-finding test employed four concentrations 1000, 100, 10 and 1 mg/L with one replicate per concentration; plus, four controls.

As a result of this range-finding test, concentrations of 1, 3.2, 10, 32, 100 mg/L were used during the definitive test.

The 72-hour EC50 was ca. 5.11 mg/L and the 72-hour NOEC was ca. 1 mg/L.

The EC50 for the substance was 5.11 mg/L and the substance is not readily biodegradable.  Therefore, the substance is classifield as aquatic toxicity category 2.

Key value for chemical safety assessment

EC50 for freshwater algae:

5.11 mg/L

EC10 or NOEC for freshwater algae:

1 mg/L