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EC number: 616-995-5 | CAS number: 8018-01-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-10-29 to 1997-11-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 24th February 1987
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Complexation products of manganese and zinc with ethylenebis(dithiocarbamate)
- EC Number:
- 616-995-5
- Cas Number:
- 8018-01-7
- Molecular formula:
- (x+y)[C4H6N2S4]2- + xMn2+ + yZn2+, x:y ranges between 1:0.062 to 1:0.12 (mean 1:0.091)
- IUPAC Name:
- Complexation products of manganese and zinc with ethylenebis(dithiocarbamate)
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Breeding facility, Jai Research Foundation, India
- Weight at study initiation: 93.0 g - 118.0 g
- Fasting period before study: 17 hours prior to dosing
- Housing: Group-housed (5 animals of same sex per cage) in polypropylene rat cage
- Diet: Rat pellet feed (Amrut brand) ad libitum (MF: Nav Maharashtra Chakan Oil Mills Limited, 43, Shaniwar Peth, Pune - 411 030, Maharashtra, India)
- Water: Pure drinking water filtered through Aquaguard water filter system ad libitum
- Acclimation period: 5 days prior to dosing
ENVIRONMENTAL CONDITIONS
- Temperature (°C): between 20 ± 2.0 and 22 ± 1.8
- Humidity (%): 76 ± 6.0
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Amount of vehicle: 10 mL/kg bw - Doses:
- 0 and 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Deaths and signs of toxicity were recorded 1, 2 and 3 hours after administration and subsequently once daily for 14 days. Individual body weights were recorded prior to dosing and on day 7 and 14.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality was observed in 2000 mg/kg body weight dose group and control group.
The oral LD50 was determined as more than 2000 mg/kg in both sexes. - Clinical signs:
- other: No toxicity symptoms were observed in any group.
- Gross pathology:
- After termination of experiment all animals from control as well as treated group were sacrificed and subjected to thorough gross pathological examination. External examination of carcass did not reveal gross lesions of significance.
In control group, lung showed mild congestion with pinpoint haemorrhages in one female animal and diffused pinpoint haemorrhages in another female animal.
In treated group, lung showed gross pathology such as diffused focal emphysema (1 male animal), mild congestion (1 female animal) and diffused ecchymotic congestion (1 female animal). Whereas, gross pathology in liver showed patchy congestion in one male animal and two female animals. Congested spleen was observed in one male animal and peritoneal adhesion in another male animal was observed in this study
In conclusion, the gross lesion recorded in lung in animal of control group taken as incidental. Whereas, in treated group the vascular pathology in lung and liver showed widespread lesions when compared with control group. Peritoneal adhesion were also recorded in one animal of this group. The severity and incidence of vascular changes in lung, liver, spleen and mesentery in treated group is a result of toxic effect of test substance.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute oral LD50 of the test item was determined to be > 2000 mg/kg bw in rats.
- Executive summary:
This study was performed to assess the acute oral toxicity of the test item to Wistar rat . The method followed was as per the guidelines of the Organization for Economic Cooperation and Development (OECD) for testing of chemicals No.401 (adopted 24th February 1987) "Acute Oral Toxicity". Two groups of rats comprising 5 males and 5 females per group were randomly selected. One group was kept as control and another group were subjected to a single oral dose of the test item at the dose level of 2000 mg/kg body weight and observed for 14 days. No mortality was observed in control and 2000 mg/kg body weight dose group. All the animals that survived at the end of the treatment period were subjected to gross pathological examination. The acute oral median lethal dose (LD50) of the test item to the rat was found to be more than 2000 mg/kg body weight.
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