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EC number: 945-909-1 | CAS number: 69415-01-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24th April 2020 to 18th September 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Physical appearance : Solid
Purity as per Certificate
of Analysis
: 60 -85% (No purity correction during dose
formulation preparation)
Total Organic Content
(TOC)
: 62.3 %
Batch No. : GRM193K01
Manufactured date : 19 July 2019
Expiry date : 03 September 2021
Physico-chemical
properties
: Density: 1.32 g/cm3 (23 °C)
Storage conditions : Ambient (+15 to +25ºC) - Analytical monitoring:
- yes
- Details on sampling:
- The alga was exposed to the test item at the nominal test concentrations of 2,
5.6, 15.7, 44 and 123.2 mg/L (respective mean measured concentrations were
0.80, 0.74, 1.31, 2.61 and 20.67 mg/L) along with a negative control and
vehicle control. Six replicates were maintained for the negative control and
vehicle control; and three replicates for the test concentrations. - Vehicle:
- yes
- Details on test solutions:
- Test medium was algal medium as described in the OECD test guideline 201
(OECD 2006) and the preparation details are given as below.
The test medium was prepared by mixing 160 mL stock solution 1 and 16 mL
each stock solution 2, 3 and 4 with 8 L of sterilized water and then making up
the volume to 16 L using sterilized water. The pH of the medium was 7.82
during definitive test. This medium was equilibrated with carbon dioxide by
keeping it in laminar hood for two hours.
Further details can be found in the attached report and appendix 1 and 2. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- American Type Culture Collection
P.O. Box 1549
Manassas, VA 20108,
USA
Culture Maintenance
Algal cells were maintained at the test facility through sub-culturing once in
5 to 10 days from the continuous culture into fresh medium in a conical flask.
These conical flasks were incubated between 21 and 25°C (±2°C) under a
white fluorescent continuous illumination of 8000 ± 1600 Lux. - Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Following parameters were observed and recorded during both range finding
test and definitive test.
• The pH of the control and the test solutions were checked at the
beginning (unused portion of test medium) and at the end of the test
(pooled test medium).
• Light intensity inside the algal chamber (at four random locations) was
measured once daily using a Lux meter.
• Maximum and minimum temperature inside the algal chamber (in the
surrogate) was recorded once daily.
• The algal biomass in each flask was determined at 24, 48 and
72 hours after the start of the test using a hemocytometer (microscopic
observation). Algal cells from at least four squares (with each 0.1 mm3
volume) in the center of slide were counted. The arithmetic mean cell
count was multiplied with 104 factor to express the cell concentration per
mL.
G19186_AGT Page 17/97
Copy No. 2/2
• The measured cell concentrations in the test cultures and controls were
tabulated together with the concentrations of the test item and the times
of measurement. - Test temperature:
- 22.0 to 23.4
- pH:
- 7.78 to 7.87
- Nominal and measured concentrations:
- Analysis of test concentrations was conducted using a validated analytical
method. Method validation and recovery of test item was carried out at 1 and
150 mg/L test concentration.
All the test concentrations along with the negative control and vehicle control
was analyzed for the test item concentration at the beginning and at the end
of test. For analysis, single composite sample was drawn from prepared test
concentrations and from the negative and vehicle control.
The criteria for acceptance of analysis results of test concentration were 80 to
120 % of nominal concentration with ≤ 20 % RSD of analyzed concentration.
The active ingredient concentration analysis showed that the per cent
agreement with nominal concentration of the samples of all test
concentrations analysed at the start and end of test were not within the range
of ± 20 % of the nominal concentration. The analysis of samples of 15.7, 44
and 123.2 mg/L drawn at the start of test; 2 and 5.6 mg/L drawn at the end of
test was repeated using back up set. Hence, all test item concentrations were
recalculated by taking mean value from the analysis results of start and end
of the test. The calculated mean measured concentrations are 0.80, 0.74, 1.31,
2.61 and 20.67 mg/L against the nominal concentration of 2, 5.6, 15.7, 44
and 123.2 mg/L, respectively. - Details on test conditions:
- Outline of the Method
Exponentially growing cultures of unicellular green alga Raphidocelis
subcapitata were exposed to various concentrations of the test item over
several generations under defined conditions. The inhibition of growth in
relation to a negative control culture was determined over a period of 72
hours.
Preparation of Inoculum Culture
To adapt and to ensure that the algae are in the exponential growth phase,
inoculum culture was prepared by inoculating Raphidocelis subcapitata of
continuous/mother culture to test medium 3 to 4 days before the start of the
test. The flasks were incubated inside the algal growth chamber under the test
conditions. The cell density of inoculum culture was 70.5 x 104 /mL during
range finding test and 121.5 x 104 /mL during definitive test.
Test Conditions
Algal cells with test concentrations were incubated in the following
conditions during range finding and the definitive test. The test containers
were plugged using cotton and randomly placed in the growth chamber. Test
containers were repositioned every day during the treatment period.
Range Finding Test
Range finding test was conducted to select the test concentrations for the
definitive test. In the range finding test the test item concentrations at 0.01,
0.1, 1, 10 and 100 mg/L was used along with negative control and vehicle
control. Each group had single replicate.
Definitive Test
The definitive test was carried out using a 2, 5.6, 15.7, 44 and 123.2 mg/L
(factor of 2.8) (respective mean measured concentrations were 0.80, 0.74,
1.31, 2.61 and 20.67 mg/L) along with negative control and vehicle control.
Preparation of Test Concentrations
To prepare test concentrations of 2 (G3), 5.6 (G4), 15.7 (G5), 44 (G6) and
123.2 (G7), 2, 5.6, 15.7, 44 and 123.2 mg test item was mixed initially with
0.1 mL tertiary butyl alcohol separately and volume was made up to 1 L in a
volumetric flask using test medium, respectively. Prepared formulation was
sonicated for 45 minutes.
After sonication of test concentration, 8.3 mL of algal cell suspension was
added to each treatment group to achieve the initial biomass of
1x104 cells/mL and was mixed thoroughly.
For negative control (G1), 8.3 mL of algal cell suspension was made up to
1 L using test medium.
For vehicle control (G2), 0.1 mL tertiary butyl alcohol was added and made
up to 1 L using test medium in a volumetric flask. 8.3 mL of algal cell
suspension was added to achieve the initial biomass of 1x104 cells/mL before
final volume make up.
Considering analytical sampling requirement all test concentrations were
repeated following above procedure and respective test concentrations were
pooled together before using it for treatment or sampling.
150 mL of prepared negative control and vehicle control was dispensed into 6
test replicates and 150 mL of each test item concentration was dispensed
separately into 3 test replicates. - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.897 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.246 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.61 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.31 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Details on results:
- At the end of the test (72 hours), the cell biomass in the test item solutions
decreased with the increase in test concentration.
The inhibition of growth rate was 0.44, 0.36, 10.12, 42.17 and 94.75% and
the inhibition of yield was 1.93, 1.57, 35.46, 84.56 and 99.64% at mean
measured concentrations of 0.80, 0.74, 1.31, 2.61 and 20.67 mg/L,
respectively, when compared to the vehicle control. There was 1.07% and
4.55% inhibition in the percent growth rate of algal biomass and yield in
vehicle control when compared with control group, respectively. Observed
cells were found morphologically normal in all the tested concentrations at
the end of test - Validity criteria fulfilled:
- yes
- Conclusions:
- The end point values for growth rate and yield 72 hours are presented below
based on mean measured concentration.
At 72 hours, values given in mg/L:
ErC50 3.897
ErC20 1.8435
ErC10 1.2465
EyC50 1.765
EyC20 1.1678
EyC10 0.9412
LOEC 2.61
NOEC 1.31
Note: 1) ErC refers to growth rate; EyC refers to yield.
2) Range mentioned below EC value refers to 95 % fiducial limits. - Executive summary:
SUMMARY
The effect of Bisphenol C Epoxywas tested on the growth of freshwater unicellular green algaRaphidocelis subcapitata(formerlyPseudokirchneriella subcapitata)for 72 hours.
The alga was exposed to the test item at the nominal test concentrations of 2, 5.6, 15.7, 44 and 123.2 mg/L (respective mean measured concentrations were 0.80, 0.74, 1.31, 2.61 and 20.67 mg/L) along with a negative control and vehicle control. Six replicates were maintained for the negative control and vehicle control; and three replicates for the test concentrations. The initial cell density of algal cells at the start of exposure was 1 x 104/mL. The cell growth was measured at 24, 48 and 72 hours after the initiation of the test using a hemocytometer (microscopic observation).
The concentration/effect relationship was determined using two factors namely growth rate and yield at the end of the test (72 hours). The inhibition of growth rate was 0.44, 0.36, 10.12, 42.17 and 94.75% and the inhibition of yield was 1.93, 1.57, 35.46, 84.56 and 99.64% at mean measured concentrations of 0.80, 0.74, 1.31, 2.61 and 20.67 mg/L, respectively, when compared to the vehicle control. There was 1.07% and 4.55% inhibition in the percent growth rate of algal biomass and yield in vehicle control when compared with control group, respectively. Observed cells were found morphologically normal in all the tested concentrations at the end of test.
The test item was recoverable at the concentrations of 1 and 150 mg/L in the vehicle. The active ingredient concentration analysis in all test concentrations showed that the mean per cent agreement with nominal concentration was 2.586 to 28.897% at the start of test and 7.815 to 102.450% at end of test (72 hour). Since the analysis results of samples drawn at the start and end of test were out of the acceptable limit (80 to 120 % of the nominal concentration with an RSD of<20%) the nominal concentration of each test concentrations were recalculated and presented as mean measured concentrations.
The end point values for growth rate and yield 72 hours are presented below based on mean measured concentration.
At 72 hours
EC values (mg/L)
ErC50
3.897
ErC20
1.8435
ErC10
1.2465
EyC50
1.765
EyC20
1.1678
EyC10
0.9412
LOEC
2.61
NOEC
1.31
Note: ErC refers to growth rate; EyC refers to yield
Reference
TABLE 1. Range Finding Test - Test Conditions
pH of Test Solutions
Group |
Treatment (mg/L) |
pH |
|
Beginning of the Test |
End of the Test |
||
G1 |
Negative control |
7.86 |
7.89 |
G2 |
Vehicle control |
7.89 |
7.92 |
G3 |
0.01 |
7.85 |
7.90 |
G4 |
0.1 |
7.88 |
7.91 |
G5 |
1 |
7.91 |
7.94 |
G6 |
10 |
7.93 |
7.97 |
G7 |
100 |
7.96 |
7.99 |
Treatment days |
Temperature (°C) |
Light intensity (lux) |
Average ± % SD |
|||
Maximum |
Minimum |
Average ± % SD |
||||
Day 1 |
22.9 |
22.0 |
22.7 ± 0.47 |
7650 |
7665 |
7646 ± 88.01 |
7652 |
7753 |
|||||
Day 2 |
23.1 |
22.4 |
7680 |
7667 |
||
7710 |
7590 |
|||||
Day 3 |
23.4 |
22.8 |
7560 |
7660 |
||
7590 |
7572 |
|||||
Day 4 |
22.8 |
22.2 |
7435 |
7600 |
||
7782 |
7765 |
TABLE 2. Range Finding Test - Average Cell Biomass
Group |
Treatment (mg/L) |
Average cell counts (× 104cells/mL) |
% Reduction at end of test |
|||
0 hour |
24 hour |
48 hour |
72 hour |
|||
G1 |
Negative control |
1 |
5.50 |
17.25 |
64 |
- |
G2 |
Vehicle control |
1 |
5.50 |
16.25 |
60 |
- |
G3 |
0.01 |
1 |
6.50 |
16.75 |
73 |
-4.78* |
G4 |
0.1 |
1 |
5.50 |
14.50 |
69 |
-3.41* |
G5 |
1 |
1 |
4.00 |
10.00 |
58 |
0.83 |
G6 |
10 |
1 |
2.75 |
4.50 |
9 |
46.33 |
G7 |
100 |
1 |
2.25 |
2.00 |
3 |
73.17 |
Note: Comparison is between test item with vehicle control group; *: To be considered as zero
Percent reduction |
= |
(Mean value of negative control) – (Value for the treatment group) |
× 100 |
Mean value of negative control |
TABLE 3. Definitive Test - Test Condition
pH of Test Solutions
Group |
Treatment (mg/L) |
pH |
|
Beginning of the Test |
End of the Test |
||
G1 |
Negative control |
7.85 |
7.87 |
G2 |
Vehicle control |
7.83 |
7.86 |
G3 |
0.8 |
7.79 |
7.83 |
G4 |
0.74 |
7.81 |
7.84 |
G5 |
1.31 |
7.83 |
7.85 |
G6 |
2.61 |
7.78 |
7.81 |
G7 |
20.67 |
7.81 |
7.84 |
Note: Respective nominal concentrations are 2, 5.6, 15.7, 44 and 123.2 mg/L
Temperature and Light Intensity Measurements of Algal Growth Chamber
Treatment days |
Temperature (°C) |
Light intensity (lux) |
Average ± % SD |
|||
Maximum |
Minimum |
Average ± % SD |
||||
Day 1 |
22.7 |
22.5 |
22.2 ± 0.18 |
7670 |
7750 |
7675 ± 85.7 |
7680 |
7810 |
|||||
Day 2 |
22.3 |
22.1 |
7690 |
7610 |
||
7710 |
7620 |
|||||
Day 3 |
22.2 |
22.0 |
7660 |
7690 |
||
7650 |
7720 |
|||||
Day 4 |
22.3 |
22.1 |
7680 |
7640 |
||
7790 |
7430 |
TABLE 4. Definitive Test Results - Algal Cell Biomass
Group No. |
Test Concentration-Mean Measured (mg/L) |
Replicate |
Average Cell Counts (x 10^4 cells/mL) |
Yield (x 10^4 cells/mL) at 72 hours |
|||
0 hour |
24 hour |
48 hour |
72 hour |
||||
G1 |
Negative Control |
R1 |
1 |
5.50 |
15.50 |
73.50 |
72.50 |
R2 |
1 |
5.75 |
16.00 |
73.50 |
72.50 |
||
R3 |
1 |
5.25 |
15.50 |
73.50 |
72.50 |
||
R4 |
1 |
6.00 |
16.50 |
73.25 |
72.25 |
||
R5 |
1 |
5.75 |
16.00 |
73.50 |
72.50 |
||
R6 |
1 |
6.00 |
16.25 |
73.00 |
72.00 |
||
Average |
1 |
5.71 |
15.96 |
73.38 |
72.38 |
||
G2 |
Vehicle Control |
R1 |
1 |
5.00 |
14.25 |
69.75 |
68.75 |
R2 |
1 |
5.00 |
14.00 |
70.50 |
69.50 |
||
R3 |
1 |
4.75 |
13.25 |
70.25 |
69.25 |
||
R4 |
1 |
5.25 |
15.00 |
70.25 |
69.25 |
||
R5 |
1 |
5.00 |
13.75 |
69.75 |
68.75 |
||
R6 |
1 |
5.00 |
15.00 |
70.00 |
69.00 |
||
Average |
1 |
5.00 |
14.21 |
70.08 |
69.08 |
||
G3 |
0.80 |
R1 |
1 |
5.25 |
12.50 |
68.75 |
67.75 |
R2 |
1 |
4.75 |
13.50 |
68.75 |
67.75 |
||
R3 |
1 |
5.00 |
13.25 |
68.75 |
67.75 |
||
Average |
1 |
5.00 |
13.08 |
68.75 |
67.75 |
||
G4 |
0.74 |
R1 |
1 |
4.75 |
13.25 |
69.25 |
68.25 |
R2 |
1 |
4.50 |
13.25 |
69.00 |
68.00 |
||
R3 |
1 |
4.50 |
13.25 |
68.75 |
67.75 |
||
Average |
1 |
4.58 |
13.25 |
69.00 |
68.00 |
||
G5 |
1.31 |
R1 |
1 |
3.25 |
9.00 |
45.00 |
44.00 |
R2 |
1 |
3.25 |
9.00 |
46.50 |
45.50 |
||
R3 |
1 |
3.00 |
8.75 |
45.25 |
44.25 |
||
Average |
1 |
3.17 |
8.92 |
45.58 |
44.58 |
||
G6 |
2.61 |
R1 |
1 |
3.25 |
5.25 |
10.75 |
9.75 |
R2 |
1 |
3.25 |
5.50 |
12.25 |
11.25 |
||
R3 |
1 |
3.25 |
5.50 |
12.00 |
11.00 |
||
Average |
1 |
3.25 |
5.42 |
11.67 |
10.67 |
||
G7 |
20.67 |
R1 |
1 |
2.25 |
1.25 |
1.25 |
0.25 |
R2 |
1 |
2.50 |
1.75 |
1.50 |
0.50 |
||
R3 |
1 |
2.50 |
1.50 |
1.00 |
0.00 |
||
Average |
1 |
2.42 |
1.50 |
1.25 |
0.25 |
Note: Respective nominal concentrations are 2, 5.6, 15.7, 44 and 123.2 mg/L
TABLE 5. Definitive Test Results - Percent Growth Rate Inhibition
Group |
Test Concentration-Mean Measured (mg/L) |
Average Cell Counts (x 10^4 cells/mL) |
Natural log (average cell counts) |
Growth rate |
Growth rate Inhibition (%) |
||
0 hours |
72 hours |
0 hours |
72 hours |
||||
G1 |
Negative Control |
1 |
73.38 |
9.21034 |
13.50599 |
0.05966 |
- |
G2 |
Vehicle Control |
1 |
70.08 |
9.21034 |
13.45998 |
0.05902 |
1.07 |
G3 |
0.8 |
1 |
68.75 |
9.21034 |
13.44082 |
0.05876 |
0.44 |
G4 |
0.74 |
1 |
69.00 |
9.21034 |
13.44445 |
0.05881 |
0.36 |
G5 |
1.31 |
1 |
45.58 |
9.21034 |
13.02981 |
0.05305 |
10.12 |
G6 |
2.61 |
1 |
11.67 |
9.21034 |
11.66736 |
0.03413 |
42.17 |
G7 |
20.67 |
1 |
1.25 |
9.21034 |
9.43348 |
0.00310 |
94.75 |
Note: Comparison is between test item with vehicle control group;
Respective nominal concentrations are 2, 5.6, 15.7, 44 and 123.2 mg/L
Percent inhibition in average specific growth rate |
= |
(Mean value for average specific growth rate in the negative control) – (Average specific growth rate for the treatment replicate) |
× 100 |
Mean value for average specific growth rate in the negative control |
TABLE 6. Definitive Test Results - Percent Yield Inhibition
Group No. |
Test Concentration-Mean Measured (mg/L) |
Average Cell Counts (x 10^4 cells/mL) |
Yield (x 10^4 cells/mL) at 72 hours |
Yield Inhibition (%) |
|
0 hour |
72 hours |
||||
G1 |
Negative Control |
1 |
73.38 |
72.38 |
- |
G2 |
Vehicle Control |
1 |
70.08 |
69.08 |
4.55 |
G3 |
0.8 |
1 |
68.75 |
67.75 |
1.93 |
G4 |
0.74 |
1 |
69.00 |
68.00 |
1.57 |
G5 |
1.31 |
1 |
45.58 |
44.58 |
35.46 |
G6 |
2.61 |
1 |
11.67 |
10.67 |
84.56 |
G7 |
20.67 |
1 |
1.25 |
0.25 |
99.64 |
Note: Comparison is between test item with vehicle control group;
Respective nominal concentrations are 2, 5.6, 15.7, 44 and 123.2 mg/L
Percent inhibition in yield |
= |
(Mean value for yield in the negative control) – (Value for yield for the treatment replicate) |
× 100 |
Mean value for yield in the negative control |
TABLE 7. Definitive Test- Specific Growth Rate: Negative Control
Replicate |
Specific Growth rate |
Replicate-mean specific growth rate |
S.D. |
% CV |
|||
0 h- 24 h |
24 h - 48 h |
48 h - 72 h |
|||||
R1 |
1.71 |
1.03 |
1.56 |
1.43 |
0.36 |
24.92 |
|
R2 |
1.75 |
1.02 |
1.53 |
1.43 |
0.37 |
26.13 |
|
R3 |
1.66 |
1.08 |
1.56 |
1.43 |
0.31 |
21.63 |
|
R4 |
1.79 |
1.01 |
1.49 |
1.43 |
0.39 |
27.51 |
|
R5 |
1.75 |
1.02 |
1.53 |
1.43 |
0.37 |
26.13 |
|
R6 |
1.79 |
1.00 |
1.50 |
1.43 |
0.40 |
27.95 |
|
Mean ± SD (% CV) |
1.74 ± 0.05 (2.87) |
1.03 ± 0.03 (2.73) |
1.53 ± 0.03 (1.92) |
1.43 ± 0 (0.12) |
- |
25.71 |
|
Mean coefficient of variation (% CV) for section by section specific growth rates |
25.71 |
||||||
The coefficient of variation (% CV) of average specific growth rates during the whole test period in replicate control cultures |
0.12 |
TABLE 8. Definitive Test- Specific Growth Rate: Treatment Group
Group |
Test Concentration-Mean Measured (mg/L) |
Replicate |
Specific Growth Rate |
||
0 h - 24 h |
24 h – 48 h |
48 h - 72 h |
|||
G2 |
Vehicle control |
R1 |
1.61 |
1.05 |
1.59 |
R2 |
1.61 |
1.03 |
1.62 |
||
R3 |
1.56 |
1.02 |
1.67 |
||
R4 |
1.66 |
1.05 |
1.54 |
||
R5 |
1.61 |
1.01 |
1.63 |
||
R6 |
1.61 |
1.10 |
1.54 |
||
Average |
1.61 |
1.04 |
1.6 |
||
SD |
0.03 |
0.03 |
0.05 |
||
% CV |
1.96 |
3.07 |
3.25 |
||
G3 |
0.80 |
R1 |
1.66 |
0.87 |
1.7 |
R2 |
1.56 |
1.04 |
1.63 |
||
R3 |
1.61 |
0.97 |
1.65 |
||
Average |
1.61 |
0.96 |
1.66 |
||
SD |
0.05 |
0.09 |
0.04 |
||
% CV |
3.11 |
8.9 |
2.17 |
||
G4 |
0.74 |
R1 |
1.56 |
1.02 |
1.66 |
R2 |
1.50 |
1.08 |
1.65 |
||
R3 |
1.50 |
1.08 |
1.65 |
||
Average |
1.52 |
1.06 |
1.65 |
||
SD |
0.03 |
0.03 |
0.01 |
||
% CV |
2.28 |
3.27 |
0.35 |
||
G5 |
1.31 |
R1 |
1.18 |
1.02 |
1.61 |
R2 |
1.18 |
1.02 |
1.64 |
||
R3 |
1.10 |
1.07 |
1.64 |
||
Average |
1.15 |
1.04 |
1.63 |
||
SD |
0.05 |
0.03 |
0.02 |
||
% CV |
4.00 |
2.78 |
1.06 |
||
G6 |
2.61 |
R1 |
1.18 |
0.48 |
0.72 |
R2 |
1.18 |
0.53 |
0.8 |
||
R3 |
1.18 |
0.53 |
0.78 |
||
Average |
1.18 |
0.51 |
0.77 |
||
SD |
0 |
0.03 |
0.04 |
||
% CV |
0 |
5.62 |
5.43 |
||
G7 |
20.67 |
R1 |
0.81 |
-0.59 |
0 |
R2 |
0.92 |
-0.36 |
-0.15 |
||
R3 |
0.92 |
-0.51 |
-0.41 |
||
Average |
0.88 |
-0.49 |
-0.19 |
||
SD |
0.06 |
0.12 |
0.21 |
||
% CV |
7.19 |
-23.99 |
-111.13 |
Note: Respective nominal concentrations are 2, 5.6, 15.7, 44 and 123.2 mg/L
Description of key information
The end point values for growth rate and yield 72 hours are presented below
based on mean measured concentration.
At 72 hours, values given in mg/L:
ErC50 3.897
ErC20 1.8435
ErC10 1.2465
EyC50 1.765
EyC20 1.1678
EyC10 0.9412
LOEC 2.61
NOEC 1.31
Note: 1) ErC refers to growth rate; EyC refers to yield.
2) Range mentioned below EC value refers to 95 % fiducial limits.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 3.897 mg/L
- EC10 or NOEC for freshwater algae:
- 1.246 mg/L
Additional information
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