Phosphonium, triphenyl(3,7,11-trimethyl-2,4,6,10-dodecatetraenyl)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

triphenyl[(2E,4E,6E)-3,7,11-trimethyldodeca-2,4,6,10-tetraen-1-yl]-λ⁵-phosphane

EC Number:

603-493-6

Cas Number:

131560-11-7

Molecular formula:

C33 H38 P

IUPAC Name:

triphenyl[(2E,4E,6E)-3,7,11-trimethyldodeca-2,4,6,10-tetraen-1-yl]-λ⁵-phosphane

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The concentration control analyses were performed with test solutions of the following nominal concentrations (mg/l) of the test substance: control (0 mg/L), 1 and 0.125 mg/L
At the start of the test (after 0 h) and at the end of the test (after 48 h) samples from vesseis without daphnids were analysed.

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was stirred in M4 medium for about 10 minutes at 20 ± 2 0C.
The nominal concentration of the stock solution was 100 mg/l.
By diluting this stock solution with M4 medium the following nominal concentrations (mg/I) were prepared: control (0 mg/L), 0.008, 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique AppIiquäe, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen.
- Justification for species other than prescribed by test guideline: not applicable
- Age at study initiation (mean and range, SD): 2 - 24 h
- Method of breeding: grouped culture
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique AppIiquäe, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen.
- Age of parental stock (mean and range, SD): 2 - 4 weeks
- Feeding during test: no

ACCLIMATION
- Acclimation period: not applicable
- Acclimation conditions (same as test or not): yes
- Type and amount of food: green algae
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not indicated

Study design

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.33 mmol/ L

Test temperature:

20.4 - 20.6 °C

pH:

8.1 - 8.2

Dissolved oxygen:

8.3 - 8.6 mg/L

Conductivity:

611 µS/cm

Nominal and measured concentrations:

nominal: 0, 0.008, 0.016, 0.031, 0.063, 0.125, 0.031, 0.25, 0.5, 1 mg/L
measured analytical concentrations at test start (0 h) in % of nominal: 48 - 70
measured analytical concentrations at test end (48 h) in % of nominal: 32 - 50

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes (nominal 20 ml)
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass, nominal 20 ml
- Volume of solution: 10 ml
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): static test, no renewal of test medium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per control (oxygen measurement): 1
- No. of vessels per control (analytical measurement): 2
- Biomass loading rate: 0.5 animals/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium according to OECD 202

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day night regime
- Light intensity: About 2 - 7 pE/(m 2.s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 0, 24 and 48 h (mobility)

VEHICLE CONTROL PERFORMED: no

Reference substance (positive control):

yes

Remarks:

reference material was tested at regular intervals

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.032 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

For the statistical calculation of the EC50 the moving average method was used.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes