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EC number: 695-938-6 | CAS number: 678966-16-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2016-01-11 to 2016-02-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch No.: 150209
Purity: 97.7 wt% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: a nominal concentration of 102 mg/L test group and a blank control containing no test substance
- Sampling time: samples were taken at 0 h, 96 h and 24 h, 48 h, 72 h (before and after the renewal) after the start of the test. - Vehicle:
- no
- Details on test solutions:
- - Preparation of test solution: 0.510 g of test substance was dissolved in a flask with some amount of test water and transferred into a 5L-jar and diluted with test water to 5000 mL to obtain the nominal concentration of 102 mg/L solution (99. 7 mg/L active constituent calculated by the 97.7% purity of test substance). The pH of test solution ranged from 3.32 to 3.41 and was adjusted to 7.6-8.0 by NaOH solution. Then the solution was stirred with a magnetic stirrer for about 24 hours to make sure test substance dissolved and used in the test directly.
- Controls: A blank control (test water without test substance) - Test organisms (species):
- other: Rare Minnow (Gobiocypris rarus)
- Details on test organisms:
- TEST ORGANISM
- Reason for selection of species: The test species is recommended by "The Guidelines for the Testing of Chemicals".
- Supplier: Institute of Hydrobiology, Chinese Academy of Sciences.
- Total number of test fish: 14
ACCLIMATION
- Acclimated for 40 days under the domestication conditions:
Water: dechlorinated tap water
Temperature: 23±2 °C
Photoperiod: 14 h light / 10 h dark
- Acclimated for 42 days under the domestication conditions:
Water: 10% dechlorinated tap water diluted with deionized water
Temperature: 23±2 °C
Photoperiod: 14 h light / 10 h dark
- Dissolved oxygen concentration: above 80% of the air saturation value
- Feeding frequency: at least 3 times per week until 24 hours before the start of the test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 19.5 mg/L (CaCO3) (duiled 10% dechorinated tap water with deionized water)
232 mg/L (CaCO3) (dechorinated tap water) - Test temperature:
- 21.0-23.0 °C
- pH:
- 7.36-7.98
- Dissolved oxygen:
- not less than 60 % of the air saturation value.
- Nominal and measured concentrations:
- Norminal concentration: 102 mg/L
- Details on test conditions:
- TEST SYSTEM
- Groups: test group and a blank group containing no test substance
- Number of fish: 7 fish / test group, 14 fish in total
- Replicate: no replicates
- Volume of test solution: 5 L / test vessel
- Loading of test fish: 7 fish / test vessel, 0.232 g fish / L
- Feeding: no feeding
OTHER TEST CONDITIONS
- Adjustment of pH: The test was performed without adjustment of pH and the pH was in the range of 7.36 to 7.98 during the test.
- Aeration: no aeration
- Photoperiod: 14 h light / 10 h darkness
Range finding study
- Test concentrations: the nominal loading rate of 9.6 mg/L WAF, 31.2 mg/L WAF, 100 mg/L WAF (Water-accommodated fraction)
- Results used to determine the conditions for the definitive study: The 96h cumulative mortalities of test fish were all 0% of the blank control and the test groups. - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality: At the end of the test, the cumulative mortalities of blank control and the 102 mg/L test group were both 0%.
- Observations on body length and weight: The average and standard deviation of test fish's body weight and total length were respectively 0.166 g ± 0.023 g and 30.43 mm ± 1. 54 mm.
- Abnormal responses: No abnormal responses were observed in blank control and the 102 mg/L test group.
- Observation of test solutions: The blank control was observed to be clear and colorless during the test period. A small amount of precipitation was observed in the 102 mg/L test group during the test period. - Results with reference substance (positive control):
- The acute toxicity test of K2Cr2O7 with Rare Minnow (Gobiocypris rarus) showed that the 24 h-LC50 of the reference substance (K2Cr2O7) was 326 mg/L to Rare Minnow (Gobiocypris rarus) and the 95% confidence limits ranged from 276 mg/L to 386 mg/L.
- Sublethal observations / clinical signs:
The measured concentrations of Lithium:
The measured concentrations of Lithium in blank control were lower than the limit of detection (LOD= 0.00104 mg/L) at 0 h, 96 h and 24 h, 48 h, 72 h (before and after the renewal) after the start of the test. The measured concentrations of Lithium in the 102 mg/L test group ranged from 2.42 mg/L to 2.981 mg/L at the start of the exposure and after the renewal in new solutions (88%-109% of the nominal concentration of Lithium). And the measured concentrations ranged from 2.49 mg/L to 2.83 mg/L before the renewal and at the end of the exposure in old solutions (91 %-103 % of the nominal concentration of Lithium). The measured concentrations of Lithium could be maintained within ±20% of the nominal concentration of Lithium at 24 h intervals.
The measured concentrations of oxalic ion:
The measured concentrations of oxalic ion in blank control were 0.039 mg/L (0 h new solution) and 0.018 mg/L (24 h old solution). The measured concentrations of oxalic ion in the 102 mg/L test group ranged from 33.2 mg/L to 38.0 mg/L at the start of the exposure and after the renewal in new solutions (96%-109% of the nominal concentration of oxalic ion). And the measured concentrations ranged from 21.4 mg/L to 34.8 mg/L before the renewal and at the end of the exposure in old solutions (61%-100% of the nominal concentration of oxalic ion). The measured concentrations of oxalic ion could not always be maintained within ±20% of the nominal concentration of oxalic ion at 24 h intervals.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentration, the median lethal concentration (96 h LC50) and the lowest concentration causing 100% mortality (96 h LC100) were more than 102 mg/L, and the highest concentration causing 0% mortality (96 h LC0) was 102 mg/L.
- Executive summary:
A determination of the acute toxicity to fish of the substance was conducted in accordance with OECD Guideline 203, and in compliance with GLP.
Groups of 7 Rare Minnow (Gobiocypris rarus)were exposed to 0 (control group),102 mg/L (a nominal concentration) the substance in 10% dechlorinated tap water for 96 h.
At the end of the test, the cumulative mortalities of blank control and the 102 mg/L test group were both 0%. No abnormal responses were observed in blank control and the 102 mg/L test group. The body weight and total length of 7 test fish in the blank control were measured when the test was finished. The average and standard deviation of test fish's body weight and total length were respectively 0.166 g ± 0.023 g and 30.43 mm ± 1.54 mm. The blank control was observed to be clear and colorless during the test period. A small amount of precipitation was observed in the 102 mg/L test group during the test period.
The measured concentrations of Lithium in blank control were lower than the limit of detection at 0 h, 96 h and 24 h, 48h, 72h (before and after the renewal) after the start of the test. The measured concentrations of Lithium in the 102 mg/L test group ranged from 2.42 mg/L to 2.981 mg/L at the start of the exposure and after the renewal in new solutions. The measured concentrations of oxalic ion in blank control were 0.039 mg/L (0 h new solution) and 0.018 mg/L (24 h old solution). The measured concentrations of oxalic ion in the 102 mg/L test group ranged from 33.2 mg/L to 38.0 mg/L at the start of the exposure and after the renewal in new solutions.
Based on the nominal concentration, the median lethal concentration (96 h LC50) and the lowest concentration causing 100% mortality (96 h LC100) were more than 102 mg/L, and the highest concentration causing 0% mortality (96 h LC0) was 102 mg/L.
Reference
Description of key information
Based on the nominal concentration, the median lethal concentration (96 h LC50) and the lowest concentration causing 100% mortality (96 h LC100) were more than 102 mg/L, and the highest concentration causing 0% mortality (96 h LC0) was 102 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 102 mg/L
Additional information
A determination of the acute toxicity to fish of the substance was conducted in accordance with OECD Guideline 203, and in compliance with GLP.
Groups of 7 Rare Minnow (Gobiocypris rarus) were exposed to 0 (control group), 102 mg/L (a nominal concentration) the substance in 10% dechlorinated tap water for 96 h.
At the end of the test, the cumulative mortalities of blank control and the 102 mg/L test group were both 0%. No abnormal responses were observed in blank control and the 102 mg/L test group. The body weight and total length of 7 test fish in the blank control were measured when the test was finished. The average and standard deviation of test fish's body weight and total length were respectively 0.166 g ± 0.023 g and 30.43 mm ± 1.54 mm. The blank control was observed to be clear and colorless during the test period. A small amount of precipitation was observed in the 102 mg/L test group during the test period.
The measured concentrations of Lithium in blank control were lower than the limit of detection at 0 h, 96 h and 24 h, 48h, 72h (before and after the renewal) after the start of the test. The measured concentrations of Lithium in the 102 mg/L test group ranged from 2.42 mg/L to 2.981 mg/L at the start of the exposure and after the renewal in new solutions. The measured concentrations of oxalic ion in blank control were 0.039 mg/L (0 h new solution) and 0.018 mg/L (24 h old solution). The measured concentrations of oxalic ion in the 102 mg/L test group ranged from 33.2 mg/L to 38.0 mg/L at the start of the exposure and after the renewal in new solutions.
Based on the nominal concentration, the median lethal concentration (96 h LC50) and the lowest concentration causing 100% mortality (96 h LC100) were more than 102 mg/L, and the highest concentration causing 0% mortality (96 h LC0) was 102 mg/L.
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