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EC number: 211-322-8 | CAS number: 638-16-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- - Factors modulating mutagenicity in microbial tests, Matsushima T, Sugimura T, Nagao M, Yahagi T, Shirai A, Sawamura M, in Short-term Test Systems for Detecting Carcinogens, Norpoth KH, Garner RC eds., Springer, Berlin-Heidelberg-New York (1980) pp.273-285
- Revised methods for the Salmonella mutagenicity test, Maron DM and Ames BN, Mutation Research 113: 173-215 (1983)
- Mutation testing using Trp reversion in Eschrichia coli, Green MHL, in Handbook of Mutagenicity Test Procedures, Kilbey BJ, Legator M, Nichols W, Ramel C eds., Elsevier, Amsterdam (1984) pp. 161-187
- Chemical Substance Research Section, Industrial Safety and Health Department, Labor Standards Bureau, Ministry of Labor Supervision: Mutagenicity study of existing chemical substances data collection based on Industrial Safety and Health Law Toxicity Investigation System, Japan Chemical Industry Ecology-Toxicology & Information Center, Tokyo, p.177 (1986) - GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- EC Number:
- 211-322-8
- EC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- Cas Number:
- 638-16-4
- Molecular formula:
- C3H3N3S3
- IUPAC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
Constituent 1
Method
- Target gene:
- S. typhimurium: histidin requirement
E. coli: tryptophan requirement
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Additional strain / cell type characteristics:
- other: UV sensitivity, film mutation (rfa), ampicillin resistance factor pKM101 (plasmid)
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix (rat)
- Test concentrations with justification for top dose:
- 313, 625, 1250, 2500 and 5000 µg/plate
- Vehicle / solvent:
- Vehicle/solvent used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: (5-nitro-2-furyl)acrylamide (AF-2), sodium azide (SA), 9-aminoacridine (9 AA), 2-aminoanthracene (2 AA)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: 1st and 2nd main experiment: pre-incubation method
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours (37°C)
NUMBER OF REPLICATIONS: triplicates in two independent experiments
DETERMINATION OF CYTOTOXICITY: growth inhibition - Evaluation criteria:
- When dosage dependence or repeatability in the absence and presence of S9 mix of more than 1 kind of assay bacteria, among the 5 kinds of assay bacteria used, was identified to be increasing, and the average value of the number of mutant colonies on the plate that includes the test material increased more than 2-fold of negative control value, it was determined (positive) as possessing gene mutagenesis in the study test system.
- Statistics:
- Statistic methodology was not used to determine the results.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- Increase of more than 2-fold of the negative control value was not identified in any of the assay bacteria used with and without S9 mix.
Deposits originated from the test material were not identified in any of the dosages used either in the absence or the presence of S9 mix.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results:
negative without metabolic activation
negative with metabolic activation
Under conditions tested, the test item did not induce gene mutations by base pair changes or frame-shifts in the genome of the tester strains used.
Thus, the test item is considered non-mutagenic in this bacterial reverse mutation assay.
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