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EC number: 431-540-9 | CAS number: 170573-32-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From June 06, 2001 to June 17, 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- regarding DOC measurements in the pre-test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- regarding DOC measurements in the pre-test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- total organic carbon (TOC) analysis
- Details on sampling:
- Analytical report
Three 400 mL beakers were prepared for TOC analysis. One was filled up with 300 mL of M4 medium (control); the two others were filled up each with 300 mL of the nominal concentrations: 10 and 20 mg/L. TOC determinations (in triplicate) were provided at 0, 24 and 48h after application. - Vehicle:
- no
- Details on test solutions:
- Based on a pre-test, the test substance was identified as poorly soluble in M4 medium. 1.25, 2.5, 5.0, 10 and 20 mg test substance were diluted in 1000 mL M4 medium and stirred (300 rpm) 24h. The test solutions were then filtered through a filter paper circle. The control was treated the same way.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Common name: Daphnia magna (Crustacea, Cladocera) (Straus), descended from a population received from "Landesumweltant (NRW)", Dusseldorf, Germany
Number of animals: 15-20 per beaker
Age: <24h
Temperature: 20 +/- 2°C
Artificial light-dark cycle of 16 to 8 hours
Feeding during test: algae (Desmodesmus subspicatus)
Holding and test medium: Elendt-Schneider M4 medium - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.2-3.2 mmol/L (total)
- Test temperature:
- 20 +/- 2°C
- pH:
- 7.5-8.5
- Dissolved oxygen:
- TOC: < 2 mg/L
- Salinity:
- -
- Conductivity:
- 500-700 µS/cm
- Nominal and measured concentrations:
- 0, 1.25, 2.5, 5.0, 10 and 20 mg/L (nominal)
- Details on test conditions:
- 50 mL glass beakers (with 30 mL) solution were used. (The test beakers were not aerated because of risk of floating daphnia to the water surface). Five daphnia were distributed per vessel for each concentration and for the control.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 7.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Analytical measurements:
Recovery of TOC in test substance preparations after 24 and 48h was 86 to 122% of TOC content of the nominal test concentrations (10 and 20 mg/L) at 0h. Therefore, the EC values were based on the nominal concentrations.
NB. The test substance preparation of 20 mg/L showed slight turbidity. After filtration all test substance concentrations were present as clear solutions.
Biological results:
The results showed that the test substance induced statistically significant immobilization to the exposed Daphnia from nominal 10 mg/L upwards within 48h. No abnormal behaviour or appearance of Daphnia was observed during the test. - Reported statistics and error estimates:
- EC50 value with 95% confidence interval, percental immobility and the NOEC after 24 and 48 h were calculated as well as statistical evaluations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the nominal 48 h EC50 and NOEC were determined to be 7.4 and 5 mg/L, respectively.
- Executive summary:
A study was conducted to determine the short term toxicity of the test substance, IsoC18 MIPA (80% active), to Daphnia magna according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. Based on the findings of a range finding test, five daphnids per concentration (5 per vessel) were exposed to a blank control and to nominal test substance concentrations of 1.25, 2.5, 10.0 and 20.0 mg/L for 48 h under static conditions. The test substance was first diluted in 1000 mL M4 medium, then stirred (300 rpm) for 24h and filtered through a filter paper circle. Test substance concentrations were verified analytically with TOC analysis at t= 0 h, t= 24 h and t=48 h. Recovery of TOC after 24 and 48 h was 86 to 122% of TOC content of the nominal test concentrations at 0 h. Therefore, the EC values were based on nominal concentrations. The test substance induced statistically significant immobilization to the exposed Daphnia from 10 mg/L. No abnormal behaviour or appearance of Daphnia was observed during the test. Under the study conditions, the nominal 48 h EC50 and NOEC were determined to be 7.4 and 5 mg/L, respectively (Wehrhahn, 2011).
Reference
None.
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 7.4 mg/L
Additional information
A study was conducted to determine the short term toxicity of the test substance, IsoC18 MIPA (80% active), to Daphnia magna according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. Based on the findings of a range finding test, five daphnids per concentration (5 per vessel) were exposed to a blank control and to nominal test substance concentrations of 1.25, 2.5, 10.0 and 20.0 mg/L for 48 h under static conditions. The test substance was first diluted in 1000 mL M4 medium, then stirred (300 rpm) for 24h and filtered through a filter paper circle. Test substance concentrations were verified analytically with TOC analysis at t= 0 h, t= 24 h and t=48 h. Recovery of TOC after 24 and 48 h was 86 to 122% of TOC content of the nominal test concentrations at 0 h. Therefore, the EC values were based on nominal concentrations. The test substance induced statistically significant immobilization to the exposed Daphnia from 10 mg/L. No abnormal behaviour or appearance of Daphnia was observed during the test. Under the study conditions, the nominal 48 h EC50 and NOEC were determined to be 7.4 and 5 mg/L, respectively (Wehrhahn, 2011).
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