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Diss Factsheets
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EC number: 200-293-7 | CAS number: 56-86-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: SIDS
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- SIDS for L-Glutamic acid
- Author:
- OECD
- Year:
- 2 013
- Report date:
- 2013
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Specific details on test material used for the study:
- 1. Name : L-glutamic acid
2. Supplier : Sigma-Aldrich Inc.
3. Purity : >=99.5%
4. Lot No. : 1371820
5. Storage condition : Stored at room temperature
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At initial exposure (0 hour) and at the end of exposure (after 48 hours), the samples from each one dish of the control and treatment groups were collected twice, and then each sample was analyzed.
Test solutions
- Details on test solutions:
- 1. Culturing and dilution: M4 medium
2. Preparation of test solutions: For preparation of stock solutions, an appropriate amount of the test substance was measured using an electronic balance and then added to mass flask filled with the dilution water (range finding test, definitive test) and then were dissolved. For the preparation of the test solutions, an appropriate amount of stock solutions was added to mass flasks filled with the dilution water (range finding test, definitive test), and then they were mixed. Each 100 mL of test solutions was prepared for test concentrations of 100, 10, 1, and 0.1 mg/L in the range finding test and for test concentration of 100 mg/L in the definitive test. The dilution water was using as the control group. The test solution was prepared on the day.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 1. Source: The 7 days old daphnids were provided from National Institute of Agricultural Science & Tech. Twenty five daphnids were kept in a glass beaker with 800 mL of dilution water.
2. Test Organism: Young daphnids, which are bone from not less than 14 days old daphnids that were above third brood progeny and less than 24 hours old, were used for this study.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20.0-20.6 °C
- pH:
- 7.24-7.87
- Dissolved oxygen:
- 6.88-7.41 mg/L
- Nominal and measured concentrations:
- - Nominal concentrations (mg/L) : control, 100
- Mean measured concentrations (mg/L) : control, 83.14 - Details on test conditions:
- 1. Experimental design: Twenty daphnids
2. Feeding and aeration : Without feeding and aeration during 48 hours of exposure period
3. Photoperiod : 16 hours of continuous artificial light and 8 hours of continuous darkness with 30 minutes dawn and dusk transition period between light and darkness - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 83.14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 83.14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Details on results:
- 1. Cumulative number of organisms immobilised and general symptoms (Table 1, 2): During the exposure period, no immobilisation and abnormal sings were found in the control group and the treatment group at 83.14 mg/L measured concentration.
2. Observation of test solutions:The test solutions in the control group and the treatment group at 83.14 mg/L measured concentration were observed for clearness prior to exposure initiation and during the exposure period. - Results with reference substance (positive control):
- The positive control study using Daphnia magna to exposure in Potassium Dichromate (Wako Pure Chemical Industries, Ltd., Japan) as a reference material for 48 hours was performed once every six months, and then EC50 was determined. The results of this study were confirmed within the range permitted for the test (mean±2SD) by historical control data in this laboratory of Biotoxtech Co., Ltd. Exposure conditions for the positive control study were in the same conditions as the definitive test.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC50 at 24 and 48 hours that was obtained under the test conditions with the test substance, L-glutamic acid was determined >83.14 mg/L, respectively. And, the No Observed Effect Concentration (NOEC) was determined ≥83.14 mg/L.
- Executive summary:
This study was designed to assess the acute toxicity of the test substance, L-glutamic acid, on the immobilisation of Daphnia magna during the exposure period of 48 hours under static test conditions and to determine the EC50 (median effect concentration) for the immobilisation of Daphnia magna and the No Observed Effect Concentration (NOEC). The nominal concentration was set at 100 mg/L, and the control group was prepared for this study. The concentration at the end of exposure exceeded (+/-) 20% of the measured initial values. Therefore, all test results were determined the geometric means. During the exposure period, the percentage of immobilisation in the control group was below 10%, and no abnormal signs such as trapping at surface of water were found. As a result of the definitive test, the percentage of immobilisation was less than 50% in the highest concentration treatment group during the exposure period, so the EC50 at 24 and 48 hours was determined >83.14 mg/L, respectively. And, the No Observed Effect Concentration (NOEC) was determined 83.14 mg/L.
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