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Diss Factsheets
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EC number: 425-560-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 AUG 1996 - 14 OCT 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The test was perfomed in compliance with GLP and the method applied follows principles descibed in the OECD Guidelines for Testing of Chemicals Ready Biodegradability - CO2 Evolution Test 301 B; Paris 1993.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): 30 mg/L dry substance from laboratory waste water treatment plants with municipal and synthetic sewage.
- Laboratory culture: The inoculum was pre-aerated two days before the start of the test. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 31.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 32.1 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: inorganic medium
- Test temperature: room temperature
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Inoculum was pre-aerated two days before the start of the test
- Measuring equipment: DOC analyser (Shimadzu TOC 5000)
SAMPLING
- Sampling frequency: Day 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27, 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes - Reference substance:
- aniline
- Preliminary study:
- Theoretical organic carbon (THOC) mg/g: 636
Theoretical CO2 production (ThCO2) mg/g: 2334 - Test performance:
- Start of the test : 30.08.1996
Test duration (days) : 28 + 1
Duration of adaptation phase (days)
Duration of degradation phase (days) : --
Validity criteria:
Maximal deviation of the degradation degree in parallel vessels: < 20%
Reference substance: ANILINE
Degradation of the reference substance (% CO2/ThCO2): 90-100 AFTER 14 DAYS
Degradation of the reference substance >60% after 14 days: YES
DIC in the blank control at 20 mg/L TOC test concentration: < 1 mg/L
CO2 production in the blank control: 30-40 mg/L
Degradation in the inhibition control (% CO2/ThCO2): 40-50
Degradation in the inhibition control after 14 days: > 25 %
Inhibition of the inoculum at the test concentration: NO
Test is valid: YES
Proof of biodegradation:
10-day-window met: NO
No pre-adapted inoculum used: YES
Test substance readily biodegradable according to OECD criteria : NO - Parameter:
- % degradation (CO2 evolution)
- Value:
- > 0 - < 10
- Sampling time:
- 28 d
- Details on results:
- sampling on days: 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27, 28
- Results with reference substance:
- Degradation of the reference substance (% CO2/ThCO2): 90-100 after 14 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The percentage biodegradation did not exceed 60 % within the 10-day window or after 28 days of incubation. The test item can therefore be considered not to be readily biodegradable.
- Executive summary:
The registered substance was tested for ready biodegradation in a CO2-evolution test according to OECD Guideline 301B following GLP.
Purpose
The purpose of this assay was to provide information on the biodegradability of the test item in aqueous environment and thus serve as a rational basis for risk assessment for environmental fate in aqueous compartments.
Study Design
The test item was investigated for its ready biodegradability in a dioxide evolution test (former Sturm test) over a period of 28 days. The biodegradation was followed measuring the production of carbon dioxide (CO2) which is a clear indication of biodegradation. The measured amount of carbon dioxide at the end of the test is compared with the calculated maximal theoretical production (ThCO2) and indicated as biodegradation degree in per cent. As the microorganisms oxidize only a part of the test substance and incorporate the rest into biomass, a degradation level >60 % is considered as sufficient biodegradation.
Result
Biodegradation
Under the test conditions the percentage biodegradation amounts to 3 % (mean value) after 28 days of incubation.
The test item can therefore be considered not to be readily biodegradable.
Biodegradation of Aniline
The reference item Aniline was sufficiently degraded to 95 % after 14 days and 96 % after 28 days of incubation thus confirming the suitability of the used activated sludge inoculum.
Biodegradation in the Toxicity Control
In the toxicity control containing both, the test item and the reference item Aniline, 42 % biodegradation was noted within 14 days and 49 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.
Conclusion
The percentage biodegradation did not exceed 60 % within the 10-day window or after 28 days of incubation. The test item can therefore be considered not to be readily biodegradable.
Reference
Description of key information
Key, CO2-evolution test, OECD 301B, GLP: not ready biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
CO2-evolution test, OECD 301B
The registered substance was tested for ready biodegradation in a CO2-evolution test according to OECD Guideline 301B following GLP.
Purpose
The purpose of this assay
was to provide information on the biodegradability of the test item in
aqueous environment and thus serve as a rational basis for risk
assessment for environmental fate in aqueous compartments.
Study Design
The test item was investigated for its ready biodegradability
in a dioxide evolution test (former Sturm test) over a period of 28
days. The biodegradation was followed measuring the production of carbon
dioxide (CO2) which is a clear indication of biodegradation.
The measured amount of carbon dioxide at the end of the test is compared
with the calculated maximal theoretical production (ThCO2) and indicated
as biodegradation degree in per cent. As the microorganisms oxidize only
a part of the test substance and incorporate the rest into biomass, a
degradation level >60 % is considered as sufficient biodegradation.
Result
Biodegradation
Under the test conditions the percentage biodegradation
amounts to 3 % (mean value) after 28 days of incubation.
The test item can therefore be considered not to be readily
biodegradable.
Biodegradation of Aniline
The reference item Aniline was sufficiently degraded to 95 %
after 14 days and 96 % after 28 days of incubation thus confirming the
suitability of the used activated sludge inoculum.
Biodegradation in the Toxicity
Control
In the toxicity control containing both, the test item and
the reference item Aniline, 42 % biodegradation was noted within 14 days
and 49 % biodegradation was determined after 28 days of incubation.
Thus, the test item can be assumed to be not inhibitory on the activated
sludge micro organisms.
Conclusion
The percentage biodegradation did not exceed 60 % within the
10-day window or after 28 days of incubation. The test item can
therefore be considered not to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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