Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 929-209-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Aug - 11 Oct 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- One sample from the freshly prepared stock solution filtrate (filtrate of 100 mg test item/L) and duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test. For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.
All samples were diluted by a factor of 2 with acetonitrile.
Additional samples of the control and the dilution solvent were taken at each sampling without any sample treatment.
All samples were stored in a freezer (≤ - 20 °C), protected from light, until analysis was performed. - Vehicle:
- no
- Details on test solutions:
- Reconstituted Water (Elendt "M4"): Analytical grade salts and additives were added at the following nominal concentrations in deionised water (conductivity < 5 μScm-1):
-------------------------------------------------------------------------------------------------------------------------
Main Compounds Trace Elements Vitamins
-------------------------------------------------------------------------------------------------------------------------
CaCl2·2 H2O 293.80 mg/L H3BO3 2860 μg/L Thiamin HCl 75.0 μg/L
MgSO4·7 H2O 123.30 mg/L MnCl2·4 H2O 361 μg/L Cyanocobalamin (B12) 1.0 μg/L
KCl 5.80 mg/L LiCl 306 μg/L Biotin (B6) 0.75 μg/L
NaHCO3 64.80 mg/L RbCl 71 μg/L
Na2SiO3·9 H2O 10.00 mg/L SrCl2·6 H2O 152 μg/L
NaNO3 0.27 mg/L NaBr 16 μg/L
KH2PO4 0.14 mg/L Na2MoO4·2 H2O 63 μg/L
K2HPO4 0.18 mg/L CuCl2·2 H2O 17 μg/L
ZnCl2 13 μg/L
CoCl2·6 H2O 10 μg/L
KI 3.3 μg/L
Na2SeO3 2.2 μg/L
NH4VO3 0.6 μg/L
Na2EDTA·2 H2O 2.5 mg/L
FeSO4·7 H2O 1.0 mg/L
-------------------------------------------------------------------------------------------------------------------------
Water Hardness: 2.5 mmol/L (= 250 mg/L) as CaCO3 Alkalinity: 0.9 mmol/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Daphnia magna
- Justification for species other than prescribed by test guideline: accepted by Guideline
- Source: in house
- Age of parental stock (mean and range, SD): 0.75 - 18 hours
- Feeding during test: no
- Food type: -
- Amount: -
- Frequency: -
ACCLIMATION
- Acclimation period: no
- Acclimation conditions (same as test or not): -
- Type and amount of food: -
- Feeding frequency: -
- Health during acclimation (any mortality observed): -
QUARANTINE (wild caught)
- Duration: -
- Health/mortality: -
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L
- Test temperature:
- 20.6 - 20.9 °C
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- 8.6 to 9.2 mg/L
- Salinity:
- -
- Conductivity:
- -
- Nominal and measured concentrations:
- nominal: 1 0.5 0.25 0.125 0.063 mg/L
analytical: 0.117 0.0615 0.033 0.0204 0.010 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.045 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic effect of the test item to Daphnia magna was assessed in a static concentration-response test. The 48-hour EC50 value was calculated to be 0.045 mg test item/L based on mean measured concentrations.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to mean measured concentrations since the concentrations of the test item were not within ± 20% of the nominal concentrations due to filtration of the stock solution.
Reference
Description of key information
OECD 202:
EC50 (48 h) = 0.045 mg/l (mean measured concentration)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.045 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.